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THE EFFECT OF STORAGE AND TRANSPORTATION CONDITIONS
OF DRIED BLOODSTAIN SAMPLES TAKEN FOR THE DIAGNOSIS OF
FABRY'S DISEASE ON THE RELIABILITY OF SCREENING RESULTS
A.N.Aripov
O.A.Aripov
B.B. Mukhammadjonov
I.R.
Oʻrinboev
Center for the Development of professional qualifications of Medical Workers,
Republican Specialized Pediatric Scientific and Practical Medical Center
https://doi.org/10.5281/zenodo.16721852
Introduction.
In modern laboratory diagnostics, various technologies are
used to analyze blood samples. Among them, the use of dried bloodstain (DBS)
samples has advantages: they are easy to assemble, easy to transport and store
for a long time. However, the reliability of the DBS sample analysis results is
directly related to how they were stored and under what conditions they were
transported [1,2,3]. This paper will consider the conditions for maintaining the
stability of DBS samples and their impact on the results of screening tests. DBS
samples are bloodstains dripping and dried on special filter paper, which are
mainly used in infant screening, infectious disease diagnosis, and
pharmacokinetic studies. This method increases safety when working with
biomaterials and simplifies logistics. However, storage and transportation of
DBS samples directly affect the reliability of the analysis results [4,5].
Materials and methods.
The optimal recommended temperature for long-
term storage of DBS samples is -20°C to +25°C. They should be stored in the
dark and dry. These conditions slow down the degradation of biological
components such as enzymes, proteins, or DNA/RNA.the arsenal and research
methods. Conversely, in DBS samples exposed to high temperatures and
humidity, biological products decompose rapidly, which can lead to an increase
in false-positive (false-positive) or false-negative (false-negative) analysis
results. It is also recommended to store samples in plastic bags along with
oxygen absorbers. It protects not only from external pollutants, but also from
oxygen and moisture in the air. However, it is advisable to use special containers
with temperature and humidity control during transportation.
Results.
Research shows that improper storage of DBS samples, especially
in newborn screening tests, has led to misdiagnosis. This leads to negative
consequences, such as involving patients in unjustified treatment, psychological
pressure, and inefficient use of resources. For this reason, medical institutions,
laboratories, and transportation services must follow strict protocols for storing
ACADEMIC RESEARCH IN MODERN SCIENCE
International scientific-online conference
184
and transporting DBS samples. Temperature and humidity control using special
equipment significantly improves the accuracy and reliability of the analysis
results.
Conclusion.
The stability of DBS samples is closely related to how they are
stored and transported, which in turn means that proper storage of DBS samples
directly affects patients' lives, ensuring the accuracy of diagnostic screening
results. That's why strict protocols, technical controls, and staff qualifications
are considered among the most important factors in this field. Thus, based on
the results obtained, it can be concluded that ensuring the stability of DBS
samples is an integral part of high—quality and reliable laboratory analysis
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