ACADEMIC RESEARCH IN MODERN SCIENCE
International scientific-online conference
36
MICROBIOLOGICAL EXAMINATION OF THE ORAL CAVITY IN
PATIENTS WITH PERIODONTITIS
Shirinova Z.H.
Shirinova H.H.
Bukhara State Medical Institute
https://doi.org/10.5281/zenodo.13378720
Abstract.
According to the results of the examination, the microbiological
state of the oral cavity of 60 patients with periodontitis and healthy periodontal
tissues consisting of 20 people were studied.
Periodontal tissue diseases are polyethological diseases, the most common
of which is chronic generalized periodontitis. The incidence rate among the
population of our country and the whole world is growing from year to year. The
study was conducted in groups of periodontal pathogenic microorganisms
L.buccalis, F.nucleatum and Fusobacterium spp., C.aureus, S.epidermis, S.mutans,
Neisseria spp. compared with the indicators of the control group, patients
confirmed the presence of symptoms of dysbiosis in the oral cavity.
Key
words:
periodontitis,
microbiological
studies,
S.mutans,
Fusobacterium spp.
The relevance of research. Periodontal pathology, being very common,
progresses with age, and by the age of 40, almost 100% of the population suffers
from it. Periodontal diseases are often asymptomatic, therefore, for their early
diagnosis and prevention, a comprehensive examination and characterization of
the state of protective factors of the oral cavity in patients is necessary. [1,2,3,6].
Periodontitis refers to inflammatory infectious periodontal diseases of a
non-specific nature. The frequency of relapses and the activity of the chronic
form of periodontitis depend on the patient's state of health. [2,4].
Despite a significant number of annual studies devoted to the problem of
periodontal diseases, both in our republic and abroad, many aspects of
pathogenesis, clinical variants of complications, their timely diagnosis and
effective prevention remain unclear, contradictory and poorly studied. [2,4,5].
The purpose of the study: to study the microbiological state of the oral
cavity in a healthy and pathological condition of periodontal tissues.
Materials and methods of research. We conducted microbiological studies
in groups, identifying the microorganisms that led to periodontal inflammation,
and comparing them with the microflora of the dental pocket of people whose
periodontal tissues were not infected.
For this purpose, 60 patients with
periodontal tissue changes and 20 people with healthy periodontitis were
examined. Laboratory methods included microbiological studies of oral fluid and
ACADEMIC RESEARCH IN MODERN SCIENCE
International scientific-online conference
37
discharge from gingival pockets. The patients were examined at the
bacteriological laboratory of the Center for Sanitary and Epidemiological
Tranquility of the Bukhara region. Saliva is collected in a sterile tube by spitting
in an amount of 1 ml; the component of the deposits of the dentoalveolar pocket
was collected with a sterile cotton swab and transferred to a sterile tube with 1
ml of saline solution. The resulting biological material was planted in an artificial
nutrient medium on gold. The method of cultural analysis was applied: 0.1 ml of
saliva, 0.1 ml of a physiological mixture, as well as a component of the gingival
papilla, all this was planted in a nutrient medium. The biological material was
planted on sterile agar in a Petri dish: the studied biological material was
pipetted over the entire surface of the agar, then incubated in a thermostat at
37oC and identified by type.
The results of the research. Microbiological examination of the exudate
from the periodontal pockets of patients in the control group revealed that it
contains gram-positive facultative anaerobic bacteria. mutants considered to be
a coagulase-negative type of staphylococcus, S. epidermidis, gram-positive
bacterium S. aureus, from Corynebacteria C xerosis, C. pseudodiphthericum,
gram-negative anaerobic bacteria Neisseria spp., Fusobacterium species and
microbial complexes containing Fusobacterium nucleatum, Veillonella parvula,
periodontopathogens
of
the
type
Bifidobacterium
dentium
and
periodontopathogens forming pigment, as well as Porphyromonas gingivalis,
alpha-hemolytic streptococci, Lactobacillus salivarius.
The microbial landscape in the periodontal pocket was studied in healthy
individuals from periodontal tissue consisting of 20 people and 11 species of
bacteria, 5 types of aerobes: S. sanguis, S.salivarius, S.epidermidis, C.xerosis,
Neisseriaspr. and 6 species of anaerobes have been identified: L.buccalis,
B.longum, L.salivarius, P.anaerobius, V.parvula, B.gingivalis.Of the total number
of microorganisms isolated in the healthy group, aerobes and facultative
anaerobes accounted for 38.6%, obligate anaerobes – 61.4%.
Conclusion. In the examined patients, 21 types of microorganisms, 9 types of
facultative anaerobes and aerobic groups were identified. The percentage of
aerobes and facultative anaerobes was 41.48%, while obligate anaerobes was
58.52%.
Literatures:
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Shirinova, H. H., & Khabibova, N. N. (2021). COMPARATIVE ANALYSIS OF
THE CLINICAL EFFICACY OF VARIOUS METHODS OF COMPLEX TREATMENT OF
CHRONIC GENERALIZED PERIODONTITIS IN OVERWEIGHT PATIENTS.Journal
ACADEMIC RESEARCH IN MODERN SCIENCE
International scientific-online conference
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