ACADEMIC RESEARCH IN MODERN SCIENCE
International scientific-online conference
123
QUANTITATIVE DETERMINATION OF TOTAL FLAVONOIDS IN
CHOLERETIC CAPSULES BASED ON DRY EXTRACT OF
SAMARKAND IMMORTELLE
Sadikova R.K.
Karieva E.S.
Tashkent Pharmaceutical Institute, Tashkent city, Republic of Uzbekistan
E-mail rano.sadikova.89@mail.ru
https://doi.org/10.5281/zenodo.15186593
Relevance:
Currently, in the range of medicinal products with choleretic,
hepatoprotective, antioxidant, anti-radiation, immunomodulatory, and other
spectra of action available in the pharmaceutical market of the Republic of
Uzbekistan, foreign preparations dominate. One way to address this issue is to
investigate new species from already studied plant genera, such as the
Samarkand immortelle. It is known that the flowers of the Samarkand
immortelle are used in traditional medicine as a choleretic and hepatoprotective
agent. The chemical composition of the Samarkand immortelle flowers has been
studied only partially. To expand the range of domestically produced choleretic
drugs, we have developed a technology for producing capsules based on the dry
extract of Samarkand immortelle.
Objective of the study:
Quantitative determination of the total flavonoids
in choleretic capsules based on the dry extract of Samarkand immortelle.
Materials and methods:
The object of the study is capsules based on a dry
extract obtained through alcohol-water extraction from the flowers of
Samarkand immortelle, recommended as a choleretic agent.
Analysis of the literature has shown that in the regulatory documentation
for Samarkand immortelle raw material, the total flavonoid content (calculated
as isosalipurposide) is used as the main biologically active substance. The
quantitative content is determined by the spectrophotometric method at a
wavelength of 315±2 nm. In this regard, it was decided to standardize the
capsules we obtained for this biologically active substance using this method.
Results:
The results of the quantitative determination of the total flavonoid
content in terms of isosalipurposide and their metrological characteristics are
presented in the table.
Table
Results of quantitative determination of the total flavonoid content in
terms of isosalipurposide in capsules, their metrological characteristics
ACADEMIC RESEARCH IN MODERN SCIENCE
International scientific-online conference
124
Content of the total
flavonoids calculated as
isosalipurposide, %
Metrological
characteristic
26,03
26,38
26,60
27,04
27,30
Х
aver
=26,67
S=0,5080 S
х
=0,2272
Т (95 %, 4)=2,78
ΔХ
aver
=0,6316
Ε=5,30 % ε
aver
= 2,36 %
The average content of total flavonoids in the dry extract, calculated as
isosalipurposide, was 26.67%.
Conclusions:
According to the obtained data, the total flavonoid content in
capsules based on dry extract of Samarkand immortelle, calculated as
isosalipurposide, is 26.67% (average of 5 determinations), with a relative error
at 95% probability = 2.36%. The standard for the content of biologically active
substances in the dietary supplement is set at 25%.
