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STUDY OF CHEMICALS IN ALLIUM MOTOR PLANT BY
EXTRACTION METHOD
Qudratov Javohir Jahongir o‘g‘li
Chirchik State Pedagogical University
Faculty of Physics and Chemistry
3rd year student of Chemistry Education
https://orcid.org/0009-0000-0623-2433
qudratovjahongirovichjavohir@gmail.com
https://doi.org/10.5281/zenodo.15356338
Abstract:
This article discusses the use of medicinal plants currently in
development and their composition. Study of vitamins contained in Allium
motor plant using chemical methods. Vitamin analysis was carried out using a
0.5% solution of acetic acid as the mobile phase in phase A and acetonitrile in
phase B in alternating mode. Chromatogram analyses of vitamins B1, B2, B6, B9,
B12, C and PP contained in Allium motor plant were carried out from standard
samples.
Keywords:
Allium motor, extraction, vitamins, natural, medicinal plants.
Introduction
Modern socio-economic changes in the world community and in Uzbekistan
require changes in the education system at all levels. They are related to the
goals, content, and results of the educational process, increasingly clearly
orienting future specialists to independence, competitiveness, creative initiative,
and mobility, which is reflected in the state educational standards for all levels
of education.
In recent years, the republic has been implementing consistent reforms in
the protection of medicinal plants, rational use of natural resources, the
organization of plantations for the cultivation and processing of medicinal
plants, and their processing. The Resolution of the President of the Republic of
Uzbekistan No. PQ-251 dated 20.05.2022 “On measures to organize the cultural
cultivation and processing of medicinal plants and their widespread use in
treatment” was adopted.
Medicinal plants are plants used for the treatment of humans and animals,
the prevention of diseases, as well as in the food, perfumery and cosmetic
industries. There are 10-12 thousand species of medicinal plants on Earth. The
chemical and pharmacological properties of more than 1,000 plant species have
been investigated. There are more than 700 species of medicinal plants in
Uzbekistan. Of these, about 120 species of plants growing in natural conditions
and cultivated are used in scientific and folk medicine. Currently, about 40-47%
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of medicines used in medicine are obtained from plant raw materials. Plants are
a living natural chemical laboratory with a complex structure, capable of
creating complex organic substances or compounds from simple inorganic
substances. The dried herb, bud, root, rhizome, tuber, bulb, bark, leaf, flower,
bud, fruit (seed), seed, juice, decoction, tea, essential oils, etc. of medicinal plants
are used as medicine.
Literature Review
Previous studies on the Allium motor plant, “Allium hydrocarbons.
Polysaccharides from Allium motor”, the dependence of the carbohydrate
content in Allium motor on the vegetation period were studied.
In the study of authentication of medicinal herbs using multispectral
analysis, the technologies of drying Allium motor plants were studied.
Information is provided about the unique, unique, medicinal properties of
Allium motor plants.
The chemical composition of Allium motor plants and its beneficial
properties for human health.[1-3]
Research methods
The Parkent region of Uzbekistan and the Chatkal mountain range and the
adjacent Kyrgyz and Kazakh regions are included. Allium motor plant belongs to
the onion family. There are many types of them. They bloom and bloom in the
spring months of April and May. The motor plant is a medicinal plant that
contains several types of vitamins. The leaves of the motor plant are dried and
then extracted.
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Figure 1 Chromatogram of standard samples of vitamins B1, B2, B6,
B9, B12, C and PP
The qualitative and quantitative parameters of vitamins B1 (47858), B2
(47864), B6 (80823-50MG), Vitamin B9 (PHR1035-1G), B12 (PHR1234-1), C
(47863) and PP (47865-U) in the finely ground samples were determined based
on standard samples from Sigma Aldrich Germany using a YSSX LC 2030 C 3D
Plus device manufactured in Japan (Shimadzu) with a PDA detector at
wavelengths of 260, 290 and 361 nm. A C18 250x4.6 mm 5 μm Precise (Perkin
Elmer) USA column was used as the stationary phase.
RESULTS
As a result of the experiments, the main weather conditions affecting the
efficiency of the solar panel were analyzed using a 0.5% solution of acetic acid as
the mobile phase and acetonitrile as the mobile phase in an alternating mode.
Table 1
Time is minute
Phase A %0.5% solution of
acetic acid in water
Phase B %
Acetonitrile
0,1
96
4
4
90
10
8
85
15
12
60
40
The flow rate was 1 ml/min, the thermostat temperature was 400C, the
injected sample volume was 10 μl, the analysis time was 14 minutes, and the
chromatograms were obtained as follows.
Table 2
Vitamin
B
1
(mg/10
0g)
Vitamin B
2
(mg/100g)
Vitamin B
6
(mg/100g)
Vitamin
B
12
(mg/100g)
Vitamin B
9
(mg/100g)
Vitamin PP
(mg/
100g)
Vitamin C
(mg/
100g)
Plant
sample
73,117
42,236
45,421
19,040
11,976
223,262
39,621
Discussion
The extraction of vitamins from the sample is carried out as follows: 1 g of
the sample (FA220 4N) was weighed with an accuracy of 0.001 mg on an
analytical balance. Then, 20 ml of 0.1 N hydrochloric acid solution was added
and mixed on a magnetic stirrer for 60 min at room temperature. The resulting
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solution was centrifuged at 12,000 rpm for 10 min and filtered through a 0.45
μm filter and placed in a vial, and the following results were obtained.
Conclusions
The following conclusions can be drawn from the above studies:
1. Allium motor is a medicinal plant and is rich in vitamins.
2. Vitamins necessary and beneficial for human organisms can be provided
by consuming natural plants.
3. The results of thermal analysis of the above samples are consistent with
the results of chemical analysis.
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