Subjects of research: White not purebred rats: offspring, transferred chronic intrauterine hypoxia; newborn children with a cerebral ischemia of a various degree and their mothers.
Purpose of work: studying molecular mechanisms of biomembranes destruction under the action of reactive oxygen species (ROS) at hypoxia and effects of water - and fat-soluble antioxidants in ultra low doses on ROS generation in the brain tissues, mitochondrial and microsomal fractions of liver at postnatal period after intrauterine hypoxia.
Methods of research: thin layer chromatography, biochemical, immuno-hystochcmical, clinical, ultrasound and cerebral blood flow evaluation, statistical.
The results obtained and their novelty: it is established the interrelation of a brain and liver, its contribution to aggravation of consequences of a cerebral ischemia at last terms after intrauterine hypoxia. It is established positive effect of phenazan in ultra low doses on the ROS overproduction and phospholipids composition misbalancc in the mitochondrial fraction of the liver and on the liver cells apoptosis too at last terms after intrauterine hypoxia. It is shown modulate effect of tocopherol on the ROS scavenger enzymes activity at early period after intrauterine hypoxia in liver microsomal fraction. It is established strong positive effect of synthetic water-soluble antioxidant phenazan on the ROS scavenger enzymes activity in the brain tissue, which cause the phospholipids composition normalization and provide optimal conditions to the brain development.
Practical value: The modified scale of a sub-optimality of the prenatal risk factors of cerebral ischemia in newborn was created, application of ultra low doses of antioxidants is proved at experimental search.
Degree of embed and economic affectivity: the received results arc introduced as methodical recommendations and information letter in The Republican Specialized Scientific - Practical Medical Centre of Obstetrics and Gynecology, department of neonatology of the Tashkent Post graduated Medical Institute, department of biochemistry of Tashkent Pediatrician Medical Institute.
Field of application: biochemistry, pediatrics.
