80
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
OPTIMIZATION OF THE TECHNOLOGY FOR HEPATOPROTECTIVE HERBAL
TEA
Sindarov N.U.
1
Kayumov F.S.
2
Tashkent Pharmaceutical Institute, Tashkent city, Republic of Uzbekistan
e-mail: nodirbeksibdarov@gmail.com
https://doi.org/10.5281/zenodo.17321314
Relevance:
In recent years, the prevalence of liver and biliary tract diseases in the Republic has
steadily increased: approximately 15–17% of the adult population suffers from various hepatic
pathologies. The etiology and pathophysiology of these diseases are complex, and their treatment
requires polyvalent agents capable of acting through several mechanisms (antioxidant, anti-
inflammatory, cytoprotective, regenerative). Herbal preparations derived from medicinal plants are
considered a promising direction in hepatoprotection. At the same time, the scientific substantiation,
optimization, and technological development of local plant resources are necessary. The present work
is devoted to creating a scientifically validated herbal composition the hepatoprotective tea
“HEPASILIMARIN”
— from local raw materials, and to evaluating its technological parameters and
quality characteristics in order to meet the pharmaceutical need for effective preparations.
The aim of the study:
To develop an optimized formulation and production technology of the
hepatoprotective herbal tea
“HEPASILIMARIN”
based on local medicinal plant raw materials, and
to determine its quality indicators and hepatoprotective potential.
Materials and methods:
Monitoring of local and cultivated medicinal plant species was
conducted (samples collected in 2022, Bostanliq district, Tashkent region). Resource-indexing and
expert ranking methods (according to A.Ya. Kobzar and O.M. Gritsenko) were used to classify and
prioritize the plants by pharmacological groups. Propensity indices were calculated for each
pharmacotherapeutic group (antioxidant, anti-inflammatory, hepatostimulant, etc.). Five
experimental mixtures (№1–№5) were prepared, and their hepatoprotective effect was assessed in
laboratory animals by measuring biochemical markers ALT and AST (µmol/s•L).
The herbal tea technology included: primary cleaning of raw materials, drying (30–90 °C depending
on species), grinding (mill, maize particle size — 3–7 mm), sieving (7 mm and 0.2 mm mesh), mixing
(mechanical mixer), and packaging into filter sachets (3.0 g).
Results:
According to resource-indexing, the calculated propensity indices (g) by
pharmacotherapeutic groups were: antioxidant — 0.8; anti-inflammatory — 0.8; hepatostimulant —
0.4; auxiliary groups (cell regeneration, fibrosis inhibitors, membrane stabilizers, vitamin carriers,
sedatives)—0.2 g each; total 3.0 g.Biochemical analysis of the experimental mixtures (№1–№5)
demonstrated that formulation №4 showed the most pronounced hepatoprotective effect: ALT = 2.6
± 0.3; AST = 1.2 ± 0.1 (µmol/s•L). Therefore, formulation №4 was selected as the basis for
“HEPASILIMARIN.”
The optimized nominal composition of
“HEPASILIMARIN”
(per sachet = 3.0 g) was:
Silybum
marianum
seeds — 1.2 g;
Mentha piperita
leaves — 0.6 g;
Zea mays
stigmas — 1.2 g. Packaging
into 3.0 g filter sachets was recommended. Quality control of the technological process and finished
product confirmed compliance with the State Pharmacopoeia (SP I, SP XI) standards: moisture — 10
± 1.2% (norm <13%), total ash — 9.25 ± 0.6% (norm <10.5%), acid-insoluble ash — 3.15 ± 0.4%
(norm <5%). Microbiological parameters and heavy metal content were within permissible limits.
Particle size distribution and absence of foreign admixtures confirmed suitability for packaging.
81
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
Conclusions.
A scientifically substantiated optimized formulation and production technology
for the hepatoprotective herbal tea
“HEPASILIMARIN”
has been developed using local medicinal
plant resources. Resource-indexing and expert ranking methods allowed the selection and
optimization of plant combinations; formulation №4 demonstrated the strongest normalizing effect
on ALT and AST values. The main quality indicators of the finished product complied with the
requirements of the State Pharmacopoeia; the tea was standardized for packaging into 3.0 g filter
sachets.
