88
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
STUDY OF THE CRYSTALLOGRAPHIC PARAMETERS OF THE ESCIN
SUBSTANCE
Sh.B. Yusupova
1
E.S. Karieva
2
Tashkent Pharmaceutical Institute, Tashkent city, Republic of Uzbekistan
e-mail: shahloyusupova@gmail.com
https://doi.org/10.5281/zenodo.17321638
Relevance:
Escin is a natural mixture of triterpene saponins consisting of α- and β-isoforms.
These isoforms differ in melting point, angle of rotation of the polarization plane, solubility,
hemolytic index, and other physical properties. According to scientific studies, it is specifically the
β-isoform of escin that possesses broad therapeutic activity, including angioprotective, anti-
inflammatory, antitumor, anti-exudative, and venotonic effects.
Research objective.
The aim of the study was to investigate certain crystallographic parameters
of escin substance (particle shape, length, width, their ratios, etc.).
Materials and methods.
The escin substance used in the research was manufactured by
KH
Health Industry Co., Ltd.
and contained 97.5–102.0% escin. Of this, 38.4% corresponded to α-escin
and 29.5% to β-escin. The substance had a specific characteristic odor and appeared as a fine white
powder with a weight loss on drying of 2.3%.
To determine the crystallographic parameters of escin, a
Motic B1-220A-3
microscope intended
for analytical work was used in combination with a
Canon A123
microscope camera. To properly
assess the size and shape of the active substance particles, photomicrographs were taken at
magnifications ranging from 26× to 900×. Measurements were carried out with an ocular micrometer
and later converted into microns. Statistical analysis of the obtained results was performed using
generally accepted criteria (Zaytsev, 1991) with a personal computer (MS Excel software).
Experiments were repeated three times.
Results.
The photomicrographs obtained in the study confirmed that the escin substance is
mainly composed of crystallites. The morphology of the particles is diverse, including rod-like,
prismatic, amorphous, and granular forms, which indicates their poorly distinguishable crystalline
structure. The size of the fragments varied: both small rounded particles and larger agglomerates with
indistinct contours were observed.
When determining the particle size of the active substance, it was found that the maximum
length ranged from
22.63–32.51 μm
, the minimum length from
7.25–13.82 μm
, with an average
length of
14.94–23.17 μm.
The maximum width of the particles was
4.72–8.54 μm
, the minimum
width
1.51–5.18 μm,
and the average width
3.12–6.86 μm
. For spherical particles, the maximum and
minimum diameters were
14.60–33.35 μm
and
1.65–7.26 μm
, respectively, with an average diameter
of
10.93–19.22 μm.
Since the ratio of average length to average width of the particles exceeded
3:1
(1:4.79; 1:3.10;
1:4.03), most of the particles had an elongated shape and were classified as anisodiametric. According
to data from the literature, substances with such particle morphology are characterized by fine
dispersity, positive compressibility, and poor flowability.
Conclusions.
Based on the results of studying the crystallographic parameters of escin
substance, it was established that the ratio of average particle length to average width exceeded
3:1.
Therefore, the majority of the particles possess an elongated morphology and belong to the group of
anisodiametric-shaped materials.
