233
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
DETERMINATION OF WATER-SOLUBLE VITAMINS IN THE ABOVE-GROUND
PART OF THE CREEPING CLOVER
Abduganieva M.F.
1
Xalilova Sh.R.
1
Urmanova F.F.
1
Sayakova G.M.
2
1
Tashkent pharmaceutical institute, Tashkent city, Republic of Uzbekistan
2
Asfendiyarov Kazakh National Medical University, Almaty city, Republic of Kazakhstan
e-mail: xalilova.shaxnoza@mail.ru
https://doi.org/10.5281/zenodo.17333391
Relevance
. it is well known that vitamins play a role in energy metabolism, the biosynthesis of
amino acids and fatty acids, photorepression processes, blood coagulation, calcium absorption, and
more. Among the water-soluble vitamins, the B-group vitamins (B1, B2, B5, B6, B9/folate, B12) and
vitamin C are excreted in urine if consumed in excess, as they are not stored in the div. Therefore,
their daily intake is essential. These vitamins play an important role in strengthening the immune
system, ensuring proper energy production, and protecting the div from various diseases.
The aim of the study:
to determine the content of water-soluble vitamins in the herb of the
creeping plant
Trifolium repens
L. (white clover) using high-performance liquid chromatography
(HPLC).
Materials and methods:
the aerial parts of the creeping plant
Trifolium repens
L. were
collected in Tashkent city in 2025 during the full flowering phase and used as the object of study. For
the determination of water-soluble vitamins using high-performance liquid chromatography (HPLC),
acetate buffer systems and acetonitrile were used as eluents (Figure 1).
Chromatographic
conditions:
HPLC system:
Agilent 1200
, equipped with an autosampler; Column:
Eclipse XDB-
C18
, 5 µm, 4.6 × 250 mm; Detector:
Diode Array Detector (DAD)
, detection at 250 nm; Flow rate:
0.8 mL/min;
Eluent composition (acetate buffer : acetonitrile): 0–5 min → 96:4; 6–8 min → 90:10;
9–15 min → 80:20; 15–17 min → 96:4; Column oven temperature:
25°C.
Figure 1.
Chromatogram of water-soluble vitamins in the aerial part of
Trifolium repens
L.
Results:
the quantitative content of water-soluble vitamins in the aerial part of
Trifolium repens
L., as determined by HPLC, is summarized in Table 1.
Table 1
№
Vitamins
Retention time
Quantity, mg/gr
1.
С (ascorbic acid)
5,356
59,3
2.
В
3
(nikotinic acid)
5,979
2,54
3.
B
1
(thiamine)
9,670
25,6
234
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
The Content of Water-Soluble Vitamins in the Aerial Part of
Trifolium repens
L.
Conclusions:
HPLC analysis of the aerial part of
Trifolium repens
L. identified ascorbic acid
(vitamin C) as the predominant water-soluble vitamin, supporting the plant’s chemical
characterization.
4.
B
6
(pyridoxine)
11,657
37,2
5.
B
9
(folic acid)
14,046
42,4
6.
B
2
(riboflavin)
15,012
39,4
