310
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
STUDYING THE ELEMENT COMPOSITION OF DRY EXTRACT FROM
TRIBULUS TERRESTRIS L.
G.Q. Umarova
Kh.M. Komilov
Tashkent Pharmaceutical Institute, Tashkent city, Republic of Uzbekistan
e-mail: gulnoza.umarova.2013@mail.ru
https://doi.org/10.5281/zenodo.17335393
Relevance:
Microelements are essential for living organisms, and numerous studies have
shown that minerals play a major role in human life. This is because without them, vital processes
cannot proceed properly. They also ensure the formation of the chemical structure of all human
tissues, including muscle tissue. It is known that plant macro- and microelements are better absorbed
by the human div. Unlike organic compounds in the human div, they are not synthesized, but are
obtained only through food. Therefore, a systematic study of the mineral composition of medicinal
plants and preparations obtained from them is of great importance in pharmacy.
Tribulus terrestris
is
a promising plant with sufficient reserves and is widely distributed in Uzbekistan. It is being studied
for the purpose of obtaining medicines and biologically active additives. In the process of analysis, it
is important to study the elemental composition of the dry extract obtained for the purpose of
developing a medicine.
The objective of the study:
to study the macro and micronutrient composition of
Tribulus
terrestris
dry extract.
Methods and techniques:
The analysis of the macro- and microelement composition of
Tribulus terrestris
dry extract was carried out by spectral method. To determine the elemental
composition, a dry extract in the amount of 0.1 g (accurate draw) was decomposed in a mixture of
nitric and perchloric acids (8 ml : 2 ml) in a Milestone microwave oven programmed with a power of
250 to 500 W at a temperature of 180 to 2200°C. The resulting solution was transferred to a 100 ml
volumetric flask, and the volume of the solution was brought to the mark with water. 10 ml of the
resulting solution was directly introduced into the spray chamber of the ICP-MS (inductively coupled
plasma mass spectrometer) AT 7500a instrument.
Equipment parameters: plasma power 1200 W, integration time 0.1 sec, peristaltic pump
rotation speed – 0.1 rpm/sec. Other parameters of the instrument were determined during the
adjustment process and remained unchanged throughout the entire maintenance period. A
multielement (27-component) standard solution with a content of 1.0 mg/l of the necessary
components was used as a standard.
Results:
The analysis revealed that the dry extract of ground blackthorn contains 50 chemical
elements. The identified elements can be arranged in the following order of decreasing abundance:
Fe > Cl > Si > S > Br > Al > P > Ba > Mn > Sr > Zn > I > Ag > Ni > K > Se > Cu > Li > Na >
Ti > Rb > Ca > W > Mg > Ir > Cd > Pd > V > Sc > U > Co > Mo > Sb > Zr > Cs > Tl > Y > Bi > In
> Rh > Nb > Hg > Au > Pt > Pb > Sn.
The presence of elements necessary for life, in particular iron, zinc, potassium, calcium,
sodium, magnesium, indicates its biological significance. Along with useful micro- and
macroelements, heavy metals - cadmium, lead, mercury - were detected in the dry extract, and their
content did not exceed the sanitary standards for dry extracts.
Conclusions:
Sodium, silicon, calcium, magnesium, potassium, sulfur, phosphorus, selenium,
iron, chromium, manganese, copper and zinc, identified in the dry extract of
Tribulus terrestris
, are
311
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
important elements that positively affect the vital activity of the organism, which determines the
biological value of the studied plant.
