386
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
STUDY OF BIOLOGICALLY ACTIVE COMPONENTS OF
PEROVSKIA
BOTSCHANTZEVII
Mullabaeva S.Kh.
Egamova F.R.
Negmatova M.U.
Siddikov D.R.
Botirov E.Kh.
Institute of Plant Chemistry named after academician S.Yu. Yunusov, AS RUz, Tashkent
e-mail: mullabayevasevara65@gmail.com, phone (99)724-29-64
https://doi.org/10.5281/zenodo.17339296
Relevance:
The growing need for medicinal products prepared on the basis of plant raw
materials is explained by their high content of biologically active compounds, good efficiency, safety,
and a wide range of therapeutic action.
Perovskia botschantzevii
Kovalevsk. & Koczk (family
Lamiacaeae) is a medicinal, essential oil, dye and honey plant growing on rocky slopes, pebbles, in
the river valleys of Kyrgyzstan, Uzbekistan, Tajikistan, and Afghanistan.
Purpose of the study
. Isolation and study of biologically active components of the roots of
Perovskia botschantzevii
collected in the Forish district of the Jizzakh region.
Materials and methods.
The crushed air-dried plant material was extracted five times at room
temperature with methanol. The combined extract was evaporated in a vacuum, the residue was mixed
with silica gel, dried and chromatographed on a silica gel column, washing successively with
gasoline, chloroform, ethyl acetate and a mixture of ethyl acetate-methanol (8:2).
Results.
Chromatography of the fractions obtained revealed seven individual natural
compounds. Based on the study of UV,
1
H and
13
C NMR spectra, the isolated substances were
identified with the diterpenoids rosmadial (1) and sujiol (2), bis-nor-diterpenoids przhevalskin (3)
and nimbiol (4), nor-ditepenoids cryptotanshinone (5) and tanshinone IIA (6), and also the ester of
caffeic acid - nepetoidin B (7). The spatial structure of rosmadial and nimbiol was confirmed by X-
ray structural analysis (XRD).
The anti-inflammatory activity of cryptotanshinone has been studied in the Institute's
Pharmacology and Toxicology Department. It has been shown that cryptotanshinone exhibits a
pronounced anti-inflammatory effect in the experiment, suppressing both the exudative and
proliferative phases of inflammation. The anti-exudative effect of cryptotanshinone has also been
revealed in the development of experimental serositis. Under the influence of cryptotanshinone,
exudation into the pleural and abdominal cavity decreases in response to intraperitoneal
administration of silver nitrate. Multiple administration of the test agent to rats with cotton balls
implanted under the skin of the back inhibited the formation of granulation-fibrous tissue around
them. In terms of the severity of its effect, cryptotanshinone in the experiments conducted showed
certain advantages over diclofenac.
Conclusions.
It has been shown that the roots of
Perovskia botschantzevii
are a rich source of
biologically active compounds and can be used to create effective medicines and dietary supplements.
