Authors

  • Farangiza Jo’rayeva
    Bukhara State Medical Institute named after Abu Ali ibn Sina

DOI:

https://doi.org/10.71337/inlibrary.uz.ijai.77674

Abstract

The influence of thyroid hormones on the formation of the brain has been shown by numerous classical studies. However, the presence of neurons, astrocytes and microglia in the brain The 2021 release of TRα and TRβ receptors was associated with low TSH levels and poorer performance on tests of cognitive development in older adults with a mean age of (49.5 ± 7.4) years, without overt thyroid dysfunction.

 

 

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INTERNATIONAL JOURNAL OF ARTIFICIAL INTELLIGENCE

ISSN: 2692-5206, Impact Factor: 12,23

American Academic publishers, volume 05, issue 03,2025

Journal:

https://www.academicpublishers.org/journals/index.php/ijai

page 1706

MORPHOFUNCTIONAL FEATURES OF THE THYROID GLAND ARE NORMAL

Jo’rayeva Farangiza Erkinovna

Bukhara State Medical Institute named after Abu Ali ibn Sina

Abstract:

The influence of thyroid hormones on the formation of the brain has been shown by

numerous classical studies. However, the presence of neurons, astrocytes and microglia in the

brain The 2021 release of TRα and TRβ receptors was associated with low TSH levels and

poorer performance on tests of cognitive development in older adults with a mean age of (49.5 ±

7.4) years, without overt thyroid dysfunction.

Key words

:thyroid gland, cerebral hypoperfusion, physical activity, cognitive abilities.

Introduction:

Our experimental studies revealed that animals with a high level of

cognitive abilities with cerebral hypoperfusion after 2–3 months in the thyroid gland there is a

greater decrease in the specific proportion of perfused perifollicular hemocapillaries and mast

cells than in animals with a low level of cognitive abilities [4,8,9]. Chronologically, these

changes coincide with the second peak of mortality in animals with a high level of cognitive

abilities, observed after bilateral ligation of the common carotid arteries [1,3].This is consistent

with the results of clinical studies that demonstrated that serum triiodothyronine levels on

admission to hospital with ischemic stroke can predict the patient's cognitive decline [7].

The aim of the study

was to assess the dynamics of structural changes in the follicular apparatus

and the hemomicrocirculatory bed of the thyroid gland in rats with cerebral hypoperfusion in rats

with different levels of learning ability under prolonged exposure to physical exertion.

Materials and methods

: The experiment was carried out on 240 rats of both sexes (120 males

and 120 females), weighing 180–200 g, Wistar strain. The thyroid gland was studied 1, 6, 8, 8,

14, 28, 35, 60 and 90 days after surgery (comparison group), as well as when it was combined

with 15 minutes of physical activity (experimental group). The animals of the experimental

group, starting from the 7th day after surgery, were subjected to forced swimming in the pool

every day for 15 minutes. In each group, two subgroups of equal size were identified: males and

females (64 in the comparison group and 56 in the experimental group), animals with low and

high levels of anxiety (64 in the comparison group and 56 in the experimental group), as well as

animals with high and low levels of cognitive functions (64 and 56 animals, respectively). The

thyroid gland was fixed in 10% neutral buffered formalin, and wiring was carried out with

intermediate Blik mixtures. On serial microtome sections (5 μm) stained with Mayer's

hematoxylin-eosin, the height of the thyroid epithelium of the follicles (Ht), the average diameter

of the follicle (Df), and the area of ​ ​ a single island of interfollicular epithelium (Si) were

determined. The specific proportion of hemocapillaries on the section (Sh) was also determined

as a percentage of the total section area. Statistical analysis of the results was carried out using

the StatSoft Statistica v6.0 software package. To assess the relationship between the studied

indicators, the Kendal correlation coefficient was used. The significance of the differences in the

measured parameters was assessed using the nonparametric Mann–Whitney test for two

independent samples. P values р<0.05 were considered significant differences.

Results and discussion:

changes in isolated cerebral hypoperfusion are characterized by

periodicity – when a short-term increase in organ activity is replaced by its decrease. Also,

results were obtained on a greater amplitude of changes in the studied parameters and a later

growth of signs of organ activity in a subgroup with a high level of cognitive abilities. A single


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INTERNATIONAL JOURNAL OF ARTIFICIAL INTELLIGENCE

ISSN: 2692-5206, Impact Factor: 12,23

American Academic publishers, volume 05, issue 03,2025

Journal:

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physical activity leads to a greater increase in the activity of the thyroid gland during cerebral

hypoperfusion, which may have a positive value, based on the ability of its hormones to

stimulate the neuroprotective mechanisms of astrocytes and shift the vector of differentiation of

progenitor cells towards neurons. Average follicle diameter comparison group Control 10,2 ± 0,7;

9,3 ± 0,8; 45,0 ± 2,2;48,5 ± 2,1 ; experimental group 1 сут. 9,4 ± 0,4 8,9 ± 0,4; 49,5 ± 2,3; 53,0 ±

2,4; 6 сут. 6,0 ± 0,3; 8,3 ± 0,3; 37,5 ± 1,5 53,3 ± 2,5 : 8 сут. 5,8 ± 0,3 7,3 ± 0,3 6,1 ± 0,2* 8,0 ±

0,3*38,5 ± 1,6 53,3 ± 2,2 43,2 ± 1,8: 58,7 ± 2,4* 14 сут. 7,4 ± 0,3 7,6 ± 0,3 6,5 ± 0,3* 6,7 ± 0,3*

41,1 ± 1,8 46,0 ± 1,9 41,9 ± 1,7* 35,4 ± 1,4* 21 сут. 9,7 ± 0,4 8,5 ± 0,3 8,4 ± 0,4* 7,9 ± 0,3*

57,7 ± 2,8 49,2 ± 2,4 40,6 ± 1,7* 39,6 ± 1,7* 28 сут. 8,6 ± 0,4 9,7 ± 0,4 9,4 ± 0,4: 10,5 ± 0,5

58,2 ± 2,4 55,4 ± 2,4 35,1 ± 1,8* 44,4 ± 1,9* 35 сут. 7,6 ± 0,3 13,4 ± 0,6 10,1 ± 0,5^ 14,6 ± 0,7*

58,6 ± 2,4 60,7 ± 2,6 30,5 ± 1,4* 39 ± 1,9*60 сут. 9,8 ± 0,5 7,6 ± 0,3 7,8 ± 0,3* 6,5 ± 0,3* 47,5

± 2,2 45,8 ± 2,2 53,3 ± 2,2* 51,7 ± 2,3^ 90 сут. 12,0 ± 0,5 10,0 ± 0,5 6,9 ± 0,3* 7,5 ± 0,4* 46,2

± 2,3 40,8 ± 1,8 32 ± 1,4* 47,9 ± 2 м The active participation of the thyroid gland, which we

revealed in the current study, not only in the development of cerebral hypoxia, but also in the

neuroprotective mechanisms caused by physical exercise in individuals with a high level of

cognitive abilities is potentially an important component of individualized therapy for ischemic

brain damage. In addition, the shift of peaks of follicular activity from a later period to an earlier

one and the elimination of intergroup heterochrony confirm our previously stated concept of

restarting adaptive-compensatory mechanisms during cerebral hypoperfusion under the influence

of physical activity

Conclusions:

physical activity against the background of cerebral hypoperfusion leads to

a restart of the adaptive-compensatory syndrome, which, in turn, is accompanied by an increase

in the activity of the thyroid epithelium in the subgroup with a high level of cognitive abilities

after 3 weeks, associated with a decrease in mortality.

REFERENCES:

1. Dyomin D.B. Effekty tireoidnykh gormonov v razvitii nervnoy sistemy (obzor) [The effects

of thyroid hormones in the development of the nervous system (review)]. Zhurnal mediko-

biologicheskikh issledovaniy [Journal of Biomedical Research], 2018, no. 6 (2), pp. 115–

127. (In Russ.; abstr. in Engl.).

2. Ivlieva A.L., Petritskaya E.N., Rogatkin D.A., Demin V.A. Metodicheskie osoben-nosti

primeneniya vodnogo labirinta Morrisa dlya ocenki kognitivnyh funkcij u zhivotnyh

[Methodical features of the application of Morris water maze for estimation of cognitive

functions in animals]. Rossiyskiy fiziologicheskiy zhurnal imeni I.M. Sechenova [Russian

physiological journal named after I.M. Sechenov], 2016, no. 102 (1), pp. 3–17. (In Russ.;

abstr. in Engl.).

3. Krishtop V.V., Pakhrova O.А., Rumyantseva T.А. Razvitie permanentnoj gipoksii

golovnogo mozga u krys v zavisimosti ot individual'nykh osobennostej vysshej nervnoj

deyatel'nosti i pola [Development of permanent brain hypoxia in rats depending on the

individual characteristics of higher nervous activity and sex]. Meditsinskiy vestnik

Severnogo Kavkaza [Medical Bulletin of the North Caucasus], 2018, vol. 13, no. 4, pp. 654–

659. (In Russ.; abstr. in Engl.).

4. Krishtop V.V., Rumyantseva T.А., Nikonorova V.G. Tipologicheskie i polovye osobennosti

morfologii shhitovidnoj zhelezy pri dlitel'noj subtotal'noj gipoksii golovnogo mozga

golovnogo mozga u krys [Typological and sex characteristics of the morphology of the


background image

INTERNATIONAL JOURNAL OF ARTIFICIAL INTELLIGENCE

ISSN: 2692-5206, Impact Factor: 12,23

American Academic publishers, volume 05, issue 03,2025

Journal:

https://www.academicpublishers.org/journals/index.php/ijai

page 1708

thyroid gland during prolonged subtotal hypoxia of the brain in rats]. Krymskiy zhurnal

eksperimental'noy i klinicheskoy meditsiny [Crimean Journal of Experimental and Clinical

Medicine], 2019, vol.9 no. 4, pp. 30–38. (In Russ.; abstr. in Engl.).

5. Krishtop V.V., Rumyantseva T.А., Pozhilov D.А. Ekspressiya GFAP v kore bol'shikh

polusharij pri razvitii tserebral'noj gipoksii u krys s razlichnymi rezul'tatami v labirinte

Morrisa [Expression of GFAP in the cerebral cortex during the development of cerebral

hypoxia in rats with different results in the Morris maze]. Biomeditsina [Biomedicine], 2020,

vol. 16, no. 1, pp. 89–98. (In Russ.; abstr. in Engl.).

6. Chrishtop V.V., Tomilova I.K., Rumyantseva T.A., et al. The effect of short-term physical

activity on the oxidative stress in rats with different stress resistance profiles in cerebral

hypoperfusion. Molecular Neurobiology, 2020, vol. 57 (7), pp. 3014–3026.

7. Huijun Chen, Yuemin Wu, Guiqian Huang, et al. Low tri-iodothyronine syndrome is

associated with cognitive impairment in patients with acute ischemic stroke: a prospective

cohort study. Am J Geriatr Psychiatry, 2018, vol. 26 (12), pp. 1222–1230.

DOI:10.1016/j.jagp.2018.07.007.

8. Lourbopoulos A., Mourouzis I., Karapanayiotides T., et al. Changes in thyroid hormone

receptors after permanent cerebral ischemia in male rats. J Mol Neurosci, 2014, vol. 54 (1),

pp. 78–91. DOI:10.1007/s12031-014-0253-3.

9. Margaill I., Royer J., Lerouet D., et al. Induction of type 2 iodothyronine deiodinase in

astrocytes after transient focal cerebral ischemia in the rat. J Cereb Blood Flow Metab, 2005,

vol. 25 (4), pp. 468–476. DOI:10.1038/sj.jcbfm.9600041.

10. Sayre N.L., Sifuentes M., Holstein D., Cheng S.Y., Zhu X., Lechleiter J.D. Stimulation of

astrocyte fatty acid oxidation by thyroid hormone is protective against ischemic

strokeinduced damage. J Cereb Blood Flow Metab, 2017, vol. 37 (2), pp. 514–527.

DOI:10.1177/0271678X16629153.

References

Dyomin D.B. Effekty tireoidnykh gormonov v razvitii nervnoy sistemy (obzor) [The effects of thyroid hormones in the development of the nervous system (review)]. Zhurnal mediko-biologicheskikh issledovaniy [Journal of Biomedical Research], 2018, no. 6 (2), pp. 115–127. (In Russ.; abstr. in Engl.).

Ivlieva A.L., Petritskaya E.N., Rogatkin D.A., Demin V.A. Metodicheskie osoben-nosti primeneniya vodnogo labirinta Morrisa dlya ocenki kognitivnyh funkcij u zhivotnyh [Methodical features of the application of Morris water maze for estimation of cognitive functions in animals]. Rossiyskiy fiziologicheskiy zhurnal imeni I.M. Sechenova [Russian physiological journal named after I.M. Sechenov], 2016, no. 102 (1), pp. 3–17. (In Russ.; abstr. in Engl.).

Krishtop V.V., Pakhrova O.А., Rumyantseva T.А. Razvitie permanentnoj gipoksii golovnogo mozga u krys v zavisimosti ot individual'nykh osobennostej vysshej nervnoj deyatel'nosti i pola [Development of permanent brain hypoxia in rats depending on the individual characteristics of higher nervous activity and sex]. Meditsinskiy vestnik Severnogo Kavkaza [Medical Bulletin of the North Caucasus], 2018, vol. 13, no. 4, pp. 654–659. (In Russ.; abstr. in Engl.).

Krishtop V.V., Rumyantseva T.А., Nikonorova V.G. Tipologicheskie i polovye osobennosti morfologii shhitovidnoj zhelezy pri dlitel'noj subtotal'noj gipoksii golovnogo mozga golovnogo mozga u krys [Typological and sex characteristics of the morphology of the thyroid gland during prolonged subtotal hypoxia of the brain in rats]. Krymskiy zhurnal eksperimental'noy i klinicheskoy meditsiny [Crimean Journal of Experimental and Clinical Medicine], 2019, vol.9 no. 4, pp. 30–38. (In Russ.; abstr. in Engl.).

Krishtop V.V., Rumyantseva T.А., Pozhilov D.А. Ekspressiya GFAP v kore bol'shikh polusharij pri razvitii tserebral'noj gipoksii u krys s razlichnymi rezul'tatami v labirinte Morrisa [Expression of GFAP in the cerebral cortex during the development of cerebral hypoxia in rats with different results in the Morris maze]. Biomeditsina [Biomedicine], 2020, vol. 16, no. 1, pp. 89–98. (In Russ.; abstr. in Engl.).

Chrishtop V.V., Tomilova I.K., Rumyantseva T.A., et al. The effect of short-term physical activity on the oxidative stress in rats with different stress resistance profiles in cerebral hypoperfusion. Molecular Neurobiology, 2020, vol. 57 (7), pp. 3014–3026.

Huijun Chen, Yuemin Wu, Guiqian Huang, et al. Low tri-iodothyronine syndrome is associated with cognitive impairment in patients with acute ischemic stroke: a prospective cohort study. Am J Geriatr Psychiatry, 2018, vol. 26 (12), pp. 1222–1230. DOI:10.1016/j.jagp.2018.07.007.

Lourbopoulos A., Mourouzis I., Karapanayiotides T., et al. Changes in thyroid hormone receptors after permanent cerebral ischemia in male rats. J Mol Neurosci, 2014, vol. 54 (1), pp. 78–91. DOI:10.1007/s12031-014-0253-3.

Margaill I., Royer J., Lerouet D., et al. Induction of type 2 iodothyronine deiodinase in astrocytes after transient focal cerebral ischemia in the rat. J Cereb Blood Flow Metab, 2005, vol. 25 (4), pp. 468–476. DOI:10.1038/sj.jcbfm.9600041.

Sayre N.L., Sifuentes M., Holstein D., Cheng S.Y., Zhu X., Lechleiter J.D. Stimulation of astrocyte fatty acid oxidation by thyroid hormone is protective against ischemic strokeinduced damage. J Cereb Blood Flow Metab, 2017, vol. 37 (2), pp. 514–527. DOI:10.1177/0271678X16629153.