Volume 02 Issue 11-2022
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VOLUME
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ABSTRACT
In pediatric coloproctology the percentage of postoperative complications do not tend to decrease. For a deeper
study of the possible causes of complications, there is a need for a morphological and histological study of the colon
wall of white laboratory rats as an alternative.
KEYWORDS
White laboratory rats, resection, large intestine.
INTRODUCTION
Modern advances in science and technology have
made it possible to develop and implement many of
the latest technologies in medicine, which has
improved the diagnosis and treatment of various
pathologies of the div, including surgical diseases of
the colon in children [1,4,13,19]. The study of the latest
literature data devoted to the study of the issues of
timely diagnosis and radical treatment of congenital
and acquired pathologies of the large intestine in
children showed that there are many different
methods of surgical treatment of these pathologies,
despite this, it remains an urgent problem [2,7,14,18].
Research Article
SOME MORPHOLOGICAL ASPECTS OF OPTIMIZATION OF COLON
RESECTION IN CHILDREN
Submission Date:
November 01, 2022,
Accepted Date:
November 08, 2022,
Published Date:
November 22, 2022
Crossref doi:
https://doi.org/10.37547/ijmscr/Volume02Issue11-08
Teshaev Sh. Zh.
Bukhara State Medical Institute2, Bukhara, Uzbekistan
Raupov F.S.
Bukhara State Medical Institute2, Bukhara, Uzbekistan
Journal
Website:
https://theusajournals.
com/index.php/ijmscr
Copyright:
Original
content from this work
may be used under the
terms of the creative
commons
attributes
4.0 licence.
Volume 02 Issue 11-2022
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VOLUME
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PAGES
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42-46
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It should be noted that among intestinal diseases in
children, the pathology of the colon occupies a
significant part, in the treatment of which surgical
correction is used to varying degrees, including partial
removal and restoration of the continuity of the
intestinal tube [2,6,9,17]. Resection of the colon in
children are forced operations. It is performed for
congenital and acquired pathologies of the colon,
including Hirschsprung disease, some forms of
anorectal
malformation,
ulcerative
necrotic
enterocolitis of newborns, familial diffuse polyposis,
etc. [2,10,12].
The application of scientific achievements in the field
of medicine has contributed to the improvement of the
diagnosis of childhood diseases, including colon
pathologies. As a result, the number of diagnosed and
operated patients increased, including the number of
postoperative complications [1,6,11,13]. In this regard,
it is of great practical importance to study the
consequences of such operations and to prevent
possible disorders that develop in the postoperative
period in children [4,7]. However, a detailed study of
the functional results of the same type of radical and
reconstructive operations often indicates the
inadequacy of the same results obtained, which
indirectly indicates the presence of possible
unaccounted for anatomical and physiological features
of the colon and the div as a whole [3,15,16].
In our opinion, the main issues that need to be
addressed when determining the effectiveness of
primary surgical intervention in these situations are: a
comprehensive assessment of the anatomical and
functional state of the colon, including structural
features of the mesenteric vessels, innervation,
physiological curves, functional segments, the state of
the sphincters of the colon intestines, etc. [6,9,12].
According to some authors [5,8,16], white laboratory
rats are often used in experimental scientific work to
study the structural foundations of the div, to study
the functional and morphological changes in the div
under the influence of various factors.
Purpose of the study:
determination of the
morphological and functional features of the colon of
experimental animals in dynamics, taking into account
the results obtained in the practice of surgical
treatment of colon pathologies in children.
MATERIAL AND METHODS
The material for histomorphological study was fresh
samples (fragments) of the colon of white laboratory
rats, in the early postnatal period of life - on the 1st, 7th,
14th and 21st days of life, and also in late postnatal
ontogenesis - at the age of 1st, 3rd, 6th and at 9
months. To study the abdominal organs of the BLK,
euthanasia was performed by an overdose of a
narcotic substance. For staining microsections
obtained from prepared histological materials, the
staining method was used - staining with hematoxylin
and eosin, for staining connective tissue and muscle
tissue according to the Van Gieson method.
In the early period of postnatal ontogenesis in
newborn rat pups, the thickness of the inner circular
muscle layer of the ascending colon in the area of the
sphincter is on average 14.9 ± 3.5 μm, and on the 21st
day of life it averaged 26.4 ± 9.7 µm. The thickness of
the outer longitudinal muscle layer averages 10.2±4.91
µm, by the age of 21 days it averaged 22.5±4.83 µm. The
total thickness of the muscle layer averages 30.2±6.04
µm, by the 21st day it averaged 54.6±10.37 µm. (see fig.
1).
In the early period of postnatal ontogenesis, the
thickness of the collagen bundles of the outer layer of
Volume 02 Issue 11-2022
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VOLUME
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the ascending colon in newborn rat pups in the area of
the sphincter is on average - 3.8 ± 2.04 µm, by the 21st
day, the thickness of the collagen bundles of the
submucosal layer averaged - 5 .5±1.47 µm. On the 21st
day of life, the thickness of the collagen bundles of the
outer layer averaged 8.0±1.48 µm, the thickness of the
collagen bundles of the submucosal layer averaged
10.7±2.2 µm. (see fig. 2).
Fig.1. Structures of the walls of the transverse colon in
3-month-old rats. Hematoxylin-eosin staining. Ob.10 x
Ok.10.
Rice. 2. Structures of the walls of the transverse colon
in 3-month-old rats. Van Gieson coloring. Ob.10 x Ok.7.
In late postnatal ontogenesis in rats of one month of
age, the thickness of the inner circular muscle layer of
the ascending part of the colon in the area of the
sphincter is on average 30.2±13.64 µm. On the 9th
month, it averaged 49.2±15.02 µm. The thickness of the
outer longitudinal muscle layer, the ascending part of
the colon in the area of the sphincter in one-month-old
white laboratory rats averages 21.8±4.12 µm. By the age
of 9 months, it averaged 33.0±10.65 µm. The total
thickness of the muscle layer of the ascending colon
averaged 59.0±17.75 µm, and at the 9th month the
total thickness averaged 87.3±22.91 µm.
In late postnatal ontogenesis, the thickness of the
collagen bundles of the outer layer of the ascending
colon in monthly BLK in the sphincter area averaged
8.6 ± 2.25 μm, the thickness of the collagen bundles of
the outer layer of the ascending colon in monthly white
laboratory rats of the submucosal layer was average -
13.9±3.15 µm. At the 9th month of life of white
laboratory rats, the thickness of the collagen bundles
of the outer layer of the ascending part of the colon
averaged 13.0±1.55 µm, the thickness of the collagen
bundles of the submucosal layer averaged 25.4±6.43
µm.
CONCLUSIONS
Connective tissue - collagen, elastic and reticular fibers
make up the submucosal basis of the walls of the colon
in the sphincter area. This basis repeats the relief of the
mucous membrane, penetrating in some places into
the depth and serves as a skeleton - the support of the
crypts, on the other hand, these fibers penetrate deep
into the muscle layer, consisting of circular and
longitudinal muscle fibers, reach the adventitia,
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forming the "retaining apparatus" of the intestine.
There is an increase in the muscular layer of the colon
in the area of the sphincter, which serves as the
anatomical basis of the sphincter formation.
Thus, when choosing the optimal level of resection or
colostomy, it is necessary to take into account the
anatomical and physiological characteristics of the
colon in children, including the localization of the colon
sphincters.
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