Abstract: In this research, the results of the effects of vincamine hydrochloride and pyrozalin iodide alkaloids on the relaxation of aortic smooth muscle cells in the presence of Ca2+ L-channels have been reported. The isometric contraction activity of the aortic blood vessel smooth muscle derived from rabbits was studied using mechanography. The results, under in vitro conditions, demonstrated that the contraction activity of the rabbit aortic blood vessel smooth muscle preparation induced by 50 mM KCl was dependent on the potential of the plasma membrane, associated with the activity of the Ca2*L-channels. It was found that vincamine and pyrozalin iodide alkaloids affected the blockage of the Ca2+i-channels and a reduction in [Ca2*] concentration. The obtained results suggest that the relaxing effect of vincamine hydrochloride and pyrozalin iodide alkaloids is based on the blockage of the L-type Ca2+ channels, and they also affect the receptor-operated Ca2+ channels. In conclusion, the results indicate that in providing the relaxing effect of vincamine and pyrozalin iodide alkaloids, the blockage of potential-dependent L-type Ca2t channels, along with the blockage of receptor-operated Ca2+ channels plays an important role.
Effect of dihydroatizin (5–250 μmol / L) on the contractile activity of rat smooth aorta muscle cells (SMC). Isometric tensile forces were recorded. Using a force transducer FT - 03 (Grass Instrument Co., USA). In it was found by experiments that the vasodilating effect of dihydroatizin mainly associated with the blockade of the Ca2 + L-channel.
Methods-Preparation of the aortic vessel muscle preparation, and recording the force of contraction.
Results- KCI-induced contraction of the aortic MMC is associated with the activation of potential–dependent Ca2+L channels of the plasma membranes of the MMC
Conclusions- The data obtained as a result of studying the mechanisms of action of the alkaloid dihydroathysine are of great practical importance and can be recommended for optimizing the process of purposeful creation of a new generation of vasorelaxant drugs.
This article presents clinical, neurological and biochemical research methods in sick children with hereditary motorsensory neuropathy. It is known that some trace elements (Ca2 +, Mg2 +, P5 +) are characterized by high energy intensity and have a high penetrating ability into the bodies of neurons through ligand-mediated receptors and sites for the cooperation of these trace elements. Against the background of pathogenetic therapy and physiotherapeutic rehabilitation measures, it is possible to limit the severity of hereditary motor-sensory neuropathy, which is especially important in pediatric neurology.
The mechanism of action of the alkaloid 1-(2´-bromine-4´,5´-dimethoxyphenyl) - 6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline (F-18) on the functional activity of smooth muscle cells of the rat aorta was studied. Isometric contraction activity was recorded using a Grass FT – 03 (Grass Instrument, USA) mechanotron. The relaxant effect of the F-18 alkaloid was found to be associated with blockade of Ca2 + (IP3R) channels in the SR, along with voltage- dependent and receptor-operated Ca2+channels in the aorta smooth muscle cell plasmalemma.