“ZAMONAVIY BIOLOGIYANING DOLZARB MUAMMOLARI VA
RIVOJLANISH ISTIQBOLLARI”
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MORPHOLOGICAL AND MOLECULAR-GENETIC IDENTIFICATION OF THE
SPECIES OF THE GENUS SCELIPHRON KLUG 1801 (HYMENOPTERA: SPHECIDAE)
Kodirov Ilxomjon Tojiakhmatovich
1
., Boymatov Odiljon Shermatovich
2
Namangan State University, Senior Lecturer of the Department of Anatomy and Physiology
1
IxlosbekIsfandiyor@gmail.
Namangan State University, Lecturer of the Department of Anatomy and Physiology
2
https://doi.org/10.5281/zenodo.15602057
Annotatsiya:
Ushbu maqolada Sceliphron avlodiga mansub
S. deforme
(F. Smith, 1856),
S.
distillatorium
(Illiger, 1807) va
S. madraspatanum
(Illiger, 1807) turlarini aniqlash bo‘yicha olib
borilgan tadqiqotlar haqida ma'lumot berilgan. Ushbu turlar Farg'ona vodiysida birinchi marta qayd
etilgan bo‘lib, ularning morfologik hamda molekulyar-genetik identifikatsiyasi amalga oshirilgan.
Kalit so‘zlar:
Hymenoptera, Sphecidae,
Sceliphron
, tur, mitoxondrianal DNA, COI.
Аннотация
:
В
данной
статье
представлена
информация
о
проведённом
исследовании
по
идентификации
видов
Sceliphron deforme
(F. Smith, 1856),
S. distillatorium
(Illiger, 1807)
и
S.
madraspatanum
(Illiger, 1807),
относящихся
к
роду
Sceliphron
.
Эти
виды
были
впервые
зарегистрированы
,
на
территории
Ферганской
долины
,
и
для
их
определения
были
проведены
морфологические
и
молекулярно
-
генетические
исследования
.
Ключевые
слова
:
Hymenoptera, Sphecidae,
Sceliphron
,
вид
,
митохондриальный
DNA, COI
Abstract:
This article provides information about the research on the identification of the
species
S. deforme
(F. Smith, 1856),
S. distillatorium
(Illiger, 1807) and
S. madraspatanum
(Illiger,
1807) belonging to the Sceliphron genus. These species were recorded for the Fergana Valley for the
first time and morphological, as well, molecular genetic identification were performed for these
species.
Key words:
Hymenoptera, Sphecidae,
Sceliphron
, species, mitohondrial DNA, COI.
Introduction
About 10,145 species of digger bees are known around the world, including 9 species belonging
to the family Heterogynaidae, 209 species to Ampulicidae, 808 species to Sphecidae, and 9,121
species to Crabronidae families.
The Apoidea (Hymenoptera: Aculeata) represents a large superfamily consisting of sphecid
wasps and bees (or “Anthophila”) [1,7,5]. Apoidea arecharacterized by a number of morphological
character states, especially in larvae [3,8]. Adult sphecid wasps usually prey on other insects or
spiders to provide food for offspring. Most sphecids are solitary except for a few species of
Crabroninae [3,8]. Most bees are pollinators of angiosperms and provide important ecosystem
functions [8]. A number of bee lineages include social insects, a feature shared with related families
of ants (Formicidae) and vespid wasps (Vespidae) [4], although the latter are mainly predators.
The genus
Sceliphron
Klug, 1801 belongs to the family Sphecidae, of which 35 species
recorded worldwide to date [11,10]. 5 species of representatives of this genus were recorded in
Central Asia, in particular, four species, such as
S. curvatum
(F.Smith, 1870),
S. deforme
(F.Smith,
1856),
S. destillatorium
(Illiger, 1807),
S. madraspatanum
(Fabricius , 1781) species were identified
in Uzbekistan: [2].
To extract DNA from the specimens, the legs of S.deforme, S.destillatorium and
S.madraspatanum belonging to the genus
Sceliphron
, which were stored in a 70% alcohol solution,
were placed on dry paper and kept at room temperature for 10-15 minutes until the alcohol
evaporated.
For DNA extraction from species of the genus
Sceliphron
, we used 2 ml of 0.5 M Tris HCl, pH
8.5, 10 ml of 2 M KCl solution, 500
μ
l of 1 M MgCl
2
, 2 ml of NP40 and 27 sucrose solution, 200 ml
deionized and autoclaved water and 2 ml proteinase K 10mg/ml. Legs of bees belonging to the genus
Sceliphron
placed in Eppendorf tubes were incubated at 65°C for 1-2 hours. After incubation,
“ZAMONAVIY BIOLOGIYANING DOLZARB MUAMMOLARI VA
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solutions of the extraction solution (Lysis buffer+protenase K) were added and kept at 92°C for 10
minutes. Then the extracted DNA samples were stored (-20°C) [6-7].
Polymerase chain reaction from isolated DNA specimens was performed using an automatic
programmable amplifier (PR-96E).
Nucleotides of COI fragments of mitochondrial DNA (mDNA) of species of the genus
Sceliphron
were isolated using LEP-F- forward, 5-attcaaccaatcataaagatat-3 and LEP-R-reverse 5-
taaacttctggatgtccaaaaa-3 primers widely used in molecular taxonomy [9,12].
The results of the conducted molecular-genetic studies show that the nucleotides with 652 pairs
of bases belonging to the COI area of the mRNA of
S. deforme
,
S. destillatorium
and
S.
madraspatanum
species belonging to the genus Sceliphron were isolated.
In order to determine their specific features, the comparative analysis of the obtained species
and the data of NCBI about S.deforme (Accession number: OM220040), S.destillatorium (Accession
number: ON505049) and S.madraspatanum (Accession number: MN148443) were conducted (Fig).
According to the results of the conducted molecular genetic research, nucleotides of the
obtained S.deforme belonging to the genus Sceliphron and S.deforme from the (NCBI) database
(Accession number: OM220040) has 100% similarity, and difference between the 83 nucleotides of
the S. destillatorium species was determined with 87.3% similarity, and 74 nucleotides difference
was found with S.madraspatanum species with 88.3% similarity.
Figure .
Comparison of the nucleotide sequence of the COI region of mRNA of species
belonging to the genus Sceliphron.
Nucleotides of the obtained S. destillatorium belonging to the genus Sceliphron and S.
destillatorium from the (NCBI) database (Accession number: ON505049) has 100% similarity, and
difference between the 70 nucleotides of the S.
madraspatanum
species was determined with 89.3%
similarity. It was noted that nucleotides of the obtained S. madraspatanum and S. madraspatanum
from the (NCBI) database (Accession number: MN148443) has 100% similarity.
Nucleotide sequences of species of the genus Sceliphron obtained from molecular genetic
studies have been deposited in the National Center for Biotechnology Information (NCBI) and
accession numbers have been obtained for the species:
S. deforme
(Accession number: PP598623),
S. destillatorium
(Accession number: PP598621),
S. madraspatanum
(Accession number:
PP598620).
“ZAMONAVIY BIOLOGIYANING DOLZARB MUAMMOLARI VA
RIVOJLANISH ISTIQBOLLARI”
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According to the results of entomological research conducted in the Ferghana Valley, the
species S
celiphron deforme
(F. Smith, 1856),
Sceliphron destillatorium
(Illiger, 1807) and
Sceliphron
madraspatanum
(Illiger, 1807) belonging to the genus
Sceliphron
were identified, and these species
were recorded for the first time in the Ferghana Valley.
The molecular genetic identification of these species was carried out and their mRNA was
studied by comparing the nucleotide sequence in the COI region. The nucleotide differences between
S
. deforme
and
S. distillatorium
species was 12.7%, with
S. madraspatanum
species 11.3%, and
10.7% differences were found between the nucleotides of the
S. distillatorium
species and
S.
madraspatanum
species.
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