Volume 03 Issue 08-2023
28
American Journal Of Applied Science And Technology
(ISSN
–
2771-2745)
VOLUME
03
ISSUE
08
Pages:
28-37
SJIF
I
MPACT
FACTOR
(2021:
5.
705
)
(2022:
5.
705
)
(2023:
7.063
)
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
ABSTRACT
In this article, Rubia tinctoru L Block diagram of obtaining a dry extract of a medicinal plant and determination of acute
toxicity. Rubia tinctoru L the plant It is used in medicine and folk economy and dyeing fabrics and materials natural
chemicals extracted from plants as paint their uses are highlighted.
Research Article
PAINTER ROYAN OBTAIN DRY EXTRACT OF THE ROOT OF THE PLANT
AND DETERMINATION OF ACUTE TOXICITY
Submission Date:
August 20, 2023,
Accepted Date:
August 25, 2023,
Published Date:
August 30, 2023
Crossref doi:
https://doi.org/10.37547/ajast/Volume03Issue08-06
A.S. Ishmuratova
Institute Of Bioorganic Chemistry Named After Academician O.S. Sodikov Of The Russian Academy Of
Sciences, Uzbekistan
B.T. Ibragimov
Institute Of Bioorganic Chemistry Named After Academician O.S. Sodikov Of The Russian Academy Of
Sciences, Uzbekistan
A.S. Toraev
Institute Of Bioorganic Chemistry Named After Academician O.S. Sodikov Of The Russian Academy Of
Sciences, Uzbekistan
J.M. Ashurov
Institute Of Bioorganic Chemistry Named After Academician O.S. Sodikov Of The Russian Academy Of
Sciences, Uzbekistan
Q.R. Baratov
Institute Of Bioorganic Chemistry Named After Academician O.S. Sodikov Of The Russian Academy Of
Sciences, Uzbekistan
T.A Khajibaev
Institute Of Chemistry Of Plant Substances Named After Academician S.Yu.Yunusov Of The Academy Of
Sciences Of The Republic Of Uzbekistan
Ax Islamov
Institute Of Bioorganic Chemistry Named After Academician O.S. Sodikov Of The Russian Academy Of
Sciences, Uzbekistan
Journal
Website:
https://theusajournals.
com/index.php/ajast
Copyright:
Original
content from this work
may be used under the
terms of the creative
commons
attributes
4.0 licence.
Volume 03 Issue 08-2023
29
American Journal Of Applied Science And Technology
(ISSN
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2771-2745)
VOLUME
03
ISSUE
08
Pages:
28-37
SJIF
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MPACT
FACTOR
(2021:
5.
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)
(2022:
5.
705
)
(2023:
7.063
)
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
KEYWORDS
Rubia tinctoru L , Rubia iberica, alizarin, anthracene, ruberythric acid, haliozin, purpurin, xanthopurpurin,
pseudopurpurin, rubiadin-glucoside, munistin, lucidin, ibericin, urinary tract stone, kidney stone, gall bladder stone,
gout, cotton, fabric, natural paint.
INTRODUCTION
Today's per day on a global scale extensive measures
are being taken to organize scientific research at a high
level in the direction of developing the creation of
effective drugs based on local raw materials and to
provide the national pharmaceutical market with
quality drugs. On the basis of the measures
implemented in this direction, a number of important
practical results are being achieved in terms of
organizing
the
development
of
competitive
preparations based on natural plant raw materials. As
it is known , it is important to study the biology of Rubia
tinctorum plant species , which have been used by
people for many years, and to separate natural
medicines from local raw materials, to prepare
inexpensive and high-quality drugs that can replace
imports , and to create the initial raw material bases.
Such preparations can be isolated from the medicinal
plant Rubia tinctorum and used in medical practice and
folk economy.
THEORETICAL PART
Dyed roan - Rubia tinctorum L. and Georgian roan
Rubia iberica C. Koch. (Rubia tinctorum L. var. Iberica
Fisch. ex DC) belongs to the Rubicaeae family. It is
found in Ukraine, Moldova, the south of the European
part of Russia, the southeast, the Caucasus
(Azerbaijan, Georgia, Armenia, Dagestan) and Central
Asia. in Uzbekistan Basically ditch on the sides , bushes
between , channels along , in the fields and in the
gardens grows [1-4]
Colorful dream The plant species is a perennial herb
with a height of 30-150 cm. The rhizome is long,
creeping, branched, cylindrical, thick, jointed, multi-
headed. The stem is covered with several, four-
pointed, jointed, spiky and looped coarse hairs. The
leaf is oval-ovate, shiny, the veins on the lower side are
covered with coarse hairs with loops, and they are
arranged in bundles of 4-6 on the stem with a very
short band. The flowers are small, greenish-yellow in
color, gathered in a semi-umbrella growing from the
axils of the leaves, forming a bag-shaped inflorescence.
The calyx is not clearly known, the corolla is 5, united,
funnel-shaped, the paternity is 5, the maternal node is
2-digit, located below. The fruit is a 1-2-seeded,
spherical, first red, later turning black wet fruit. It
blooms in June-August, the fruit ripens in August-
September. Rubia tinctorum .
Volume 03 Issue 08-2023
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VOLUME
03
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Pages:
28-37
SJIF
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(2021:
5.
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)
(2022:
5.
705
)
(2023:
7.063
)
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
Figure 1. Colorful dream Above ground and below (root) part of the plant
Colorful dream The finished product of the harvested
plant consists of rhizomes and root pieces. The
thickness of rhizome pieces depends on the year they
were dug up from the ground. One-year-olds are thin,
two-year-olds are thick, and they get thicker every
year. The upper side is reddish brown. When it is cut
crosswise, the bark layer is red-brown, and the wood
part is red. The product has a characteristic weak smell,
sweeter at first, and then a slightly sour and bitter
taste. The rhizome turns the water brownish-red. The
underground part (root) of the Rubia tinctorum plant
is shown to be thin in the first year and to grow in the
second and third year (Fig. 2).
Figure 2. Colorful dream The root of the plant is thin in the first year and grows in the second and third year
Colorful dream The moisture content of the product
harvested from the plant is 13%, total ash is 10%, other
parts of the plant (stems, leaves, etc.) are 1.5%, organic
compounds are 1% and mineral compounds are more
than 1%, the amount of anthraglycosides (combined as
glycosides) in the product is 3 It should not be less than
% [5-7] .
rhizome of Rubia tinctorum contains up to 5-6%
anthracene compounds (alizarin, ruberythric acid,
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VOLUME
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SJIF
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(2021:
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(2022:
5.
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(2023:
7.063
)
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
galiosin, purpurin, xanthopurpurin, pseudopurpurin,
rubiadin-glucoside, munistin, lucidin, ibericin, etc.).
Anthracene Alizarin Ruberythric acid
Purpurin Pseudopurpurin lucidin
In addition to anthracene products, organic acids in plant roots contain up to 15% sugars, proteins, pectin substances,
ascorbic acid , and citric, malic, and tartaric acids. occurs.
ascorbic acid citric acid malic acid tartaric acid
The root of Rubia tinctorum plant contains carbohydrates, phenolic acids and their derivatives, coumarin,
anthraquinone, triterpenoids, flavonoids (quercetin, kaempferol, apigenin).
antiroquinone quercetin kaempferol apigenin
Colorful dream In medicine, the plant has antispasmodic and diuretic effects, as well as softening of kidney stones
(phosphates). Therefore, medicinal preparations are used for ureteral stones, kidney stones, gallstones and gout . [5-
7]
Volume 03 Issue 08-2023
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VOLUME
03
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Pages:
28-37
SJIF
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(2021:
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(2022:
5.
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)
(2023:
7.063
)
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
Colorful dream In addition to the medicinal use of the plant, dyeing with natural chemicals extracted from the plant
as a natural dye for the dyeing of threads, glam, fabrics, and materials grown in our Republic, which leads to an
increase in work hours [5-7], ( Figure 3).
Figure 3 . Colorful dream yarn dyed with natural chemicals extracted from plants
DISCUSSION OF RESULTS
Today's in the day a new product developed in the field
of pharmaceuticals and medicine through a scientific
approach based on natural medicinal plants in the
world if a bioactive additive ( BFQ) is created and put
into production and jobs are created, the number of
unemployed is reduced and Satisfying the internal
demand of our republic and creating the opportunity
to export abroad according to many our scientists
scientific research are taking Colorful dream plant in
cooperation with scientists of the Institute of
Bioorganic Chemistry named after Academician O.S.
Sodikov Experiments were carried out in the spray
drying extractor "ZPG 150" (XPR) model , which allows
drying 200 l of solution per hour in the scientific and
technological center adapted to GMP conditions,
which was launched under the experimental
production enterprise of the Institute of Chemistry of
Plant Substances of the Russian Federation .
Experiments were carried out as follows. Paint coating
on the extraction device plant 10.0 kg was crushed into
4-6 mm size , and 100.0 l (hydromodule 1:10) of 80%
etonol was poured , then water vapor was injected into
the "steam jacket" of the extractor, heated to a
temperature of 60 C and left for 24 hours. The extract
is drawn from the lower part of the extractor by a
specially installed pump, driven in a specially equipped
rotor equipment with 80% ethanol , and dyed. Aqueous
extract of the plant was collected and poured. The
extraction process was carried out under the same
conditions as the first one . For this purpose, another
100.0 l (hydromodule 1:10) of 80% etonol was prepared
from the extracted ethanol and drained from the
upper part of the extractor again by raining. extraction
was performed three times in this way. At the end of
the extraction process, the extract (60, l) was poured
and the results were analyzed (Table 1).
1 - table
Rubia tinctorum plant with 80% ethanol at 60 C
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VOLUME
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(2023:
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1121105677
Publisher:
Oscar Publishing Services
Servi
Number
of extract
infusions
Raw material
weight kg
Extraction
time, hours
Raw
materials
and 80%
ethanol
80% ethanol extract
(ml)
Substance %
1
10
24
1:10
100
-
2
again
24
1:10
100
-
3
again
24
1:10
100
total
10
24
1:10
300
16.9
Production and drying of Rubia tinctorum dry extract
technological general block sx was developed . Rubia
tinctorum plant is crushed in a mill (1) in size of 4-6 mm,
weighed on a scale (2), placed in an extractor (5) in the
amount of 10 kg, and 100 l (hydromodule 1:10) of 80%
etonol is placed in a measuring cup (3, 4) for 24 hours.
was kept for a day. In this method, the raw material
was extracted 3 times. The resulting 280 l extract was
filtered on a notch filter (8) and collected in a vessel (9)
and condensed in a vacuum evaporator (10) until 25 l,
i.e. 15% dry residue, and cooled (12). Then (13) was
poured into a vessel and spray-dried in an Anhydro No.
2 (Denmark) device . It was dried in a spray dryer with
hot air inlet at 170°C, outlet at 80°C, air pressure 0.2
MPa for 50 minutes (RS-12). The dry extract substance
(M-16) with a content of not less than 16-17% of the
obtained product was crushed and packaged as a
finished product. The technological process and device
scheme for extracting dry extract from the plant was
developed (Scheme 1).
Scheme 1
" Rubia tinctorum ".
Volume 03 Issue 08-2023
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VOLUME
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ISSUE
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Pages:
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SJIF
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(2022:
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)
(2023:
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OCLC
–
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Publisher:
Oscar Publishing Services
Servi
(1-mill, 2-scale, 3,4- (60°C 80% etonol ) container, 5,6,7-extractor, refrigerator, cart for shot, 8-filter, 9-container for
filtered extract, 10 -vacuum evaporator equipment, 11-container for extracted solvent, 12-(for settling) separation
funnel, 13-container, RS-12 spray dryer, M-16 grinder, 17- drying cabinet .
Colorful dream the appearance of the substance obtained by the extraction method of the plant root extract in 80%
ethanol in a spray drying extractor (Fig. 4).
Figure 4. Colorful dream the root of the plant and the substance of its extract in 80% ethanol.
Colorful dream to determine the acute toxicity of the
root and dry substance of the plant The pharmacology
and screening of biologically active compounds were
examined in the laboratory of the Institute of
Bioorganic Chemistry of the Russian Federation FA .
Samples for research were presented in powder form.
Studies on the determination of acute toxicity dye
royan The samples of the root and dry substance of the
plant were taken. Experiments were performed on
male white laboratory mice with a div weight of
20±2.0 g. Research was conducted in a generally
accepted way. Each sample was studied at 5 different
doses, 6 mice were taken per group.
All pharmacological studies were performed in healthy,
sexed, quarantined mice for 14 days. The samples were
injected into the stomach of mice in doses of 500, 1000,
1500, 2000 and 3000 mg/kg using a special probe. The
animals of the control group were given 0.5 ml of
distilled water. On the first day of the experiment, the
animals of the study and control groups were
monitored in the laboratory for hourly general
condition, observable tremors, and mortality as
indicators of functional status. Then, for 2 weeks, in
vivarium conditions, all groups were examined daily for
the general condition, activity, fur coat, skin condition,
rate and depth of breathing, urine output, div weight
change and other indicators. All experimental animals
were kept on the same routine diet, with unlimited
access to food and water. [8,9].
At the end of the experiment, the average lethal dose
(LD 50 ) and toxicity class of the tested drug were
determined [10] .
Statistical calculation of the obtained results It was
conducted on the basis of Windows XP (Excel)
software.
Colorful dream root and dry substance of the plant
general effects and acute toxicity were conducted in
purebred white laboratory mice. The generally
accepted method was used to determine the
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Pages:
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SJIF
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(2023:
7.063
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OCLC
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1121105677
Publisher:
Oscar Publishing Services
Servi
parameters of acute poisoning. The studied substance
was injected into the stomach using a special probe in
doses of 500, 1000, 1500, 2000 and 3000 mg/kg. The
animals of the control group were given 0.5 ml of
distilled water. On the first day of the experiment, the
general condition of the animals was monitored hourly
in the laboratory, where the survival rate, general
condition, possible shivering and death during the
experiment were monitored in the laboratory as
indicators of their functional status. After that, for 2
weeks in the vivarium of the Institute of Bioorganic
Chemistry, the general state and activity of the animals
in all groups, the specific states of their behavior, the
depth and speed of breathing, the condition of
feathers, the skin cover, and the changes in div
weight were monitored every day. All animals were
kept in the same vivarium conditions on a common
diet, water and food were not restricted.
Colorful dream root and dry substance of the plant
500, 1000, 1500, 2000 and 3000 mg/kg doses studied in
the aforementioned animal parameters effects of
acute poisoning were not observed. No animal deaths
were observed at the above doses observed
throughout the experiment. Animals in the
experimental groups did not show any decrease in
div weight when compared to the control group .
Based on the obtained results, we can conclude that
Boyokdar royan It was determined that the average
lethal dose (LD 50 ) of the root and dry substance of
the plant was >3000mg/kg when it was injected into
the stomach of mice. The obtained results were
analyzed (Table 2).
Table 2
Colorful dream Indications of acute poisoning when the root and dry substance of the plant are injected into the
stomach of mice
Groups
Animal
type, sex
Dose a
mg/kg ,
ml
Number
of
animals/
dead
animals
in the
group
Average
animal
mass
(1 day)
Average
animal
mass
(14 days)
LD
50
with
confidenc
e interval
g
LD
84
Colorful dream
The dry substance
of the root of the
plant
The
mouse is
male
10 00
6/0 _
22
25
> 500 0
mg/kg
2000
6/0 _
20
23
30 00
6/0 _
21
24
40 00
6/0 _
20
24
50 00
6/0 _
21
25
Colorful dream
the
root of the plant is
crushed
10 00
6/0 _
20
24
2000
6/0 _
21
24
30 00
6/0 _
20
23
40 00
6/0 _
21
25
Volume 03 Issue 08-2023
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American Journal Of Applied Science And Technology
(ISSN
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VOLUME
03
ISSUE
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Pages:
28-37
SJIF
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MPACT
FACTOR
(2021:
5.
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)
(2022:
5.
705
)
(2023:
7.063
)
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
The
mouse is
male
50 00
6/0 _
21
24
Control
The
mouse is
male
0.5
6/0 _
21
25
Colorful dream The results of the study of the acute
toxicity of the root and dry substance of the plant
showed that in mice that the average lethal dose (LD
50 ) after a single gastric injection is higher than 3000
mg/kg and the samples are non-toxic It was found that
it belongs to the class of compounds.
CONCLUSION
1. A colorful dream The protective substance was
obtained by extracting the extract of the root of the
plant in 80% ethanol in a spray-drying extractor .
2. Paint finish The results of the study of the acute
toxicity of the root and dry substance of the plant
showed that in mice that the average lethal dose (LD
50 ) after a single gastric injection is higher than 3000
mg/kg and the samples are non-toxic It was found that
it belongs to the class of compounds.
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MPACT
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(2021:
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(2022:
5.
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)
(2023:
7.063
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