The aim of the research work is a detail characterization of the process of glutathione release from cells in normal conditions and under hypoosmotic stress, and elucidation of the role of ion channels and transporters in this process.
Scientific novelty of the research work is as follows:
in this study, for the first time, the process of release of glutathione from thymocytes in normal conditions and under hypooosmotic stress has been demonstrated;
using specific pharmacology, it was demonstrated for the first time that glutathione is released predominantly via volume-sensitive anion channel, whereas anion transporters of SLCO/OATP and SLC22A/OAT play a secondary role.
it was shown that activation of adenylyl cyclase system causes a negative effect on the release of glutathione from thymocytes.
the presence of the osmo-reactive transport of glutathione was also found in other types of cells (erythrocytes and melanoma), and the modulation of the cell volume regulation by extracellular glutathione was shown.
