492
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
ANTICOAGULANT PROPERTIES OF ENDOPHYTIC FUNGI FROM CERTAIN
MEDICINAL PLANTS OF THE CHATKAL BIOSPHERE RESERVE
Boʻrieva M.R.
Nuralieva X.A.
Tashkent Pharmaceutical Institute, Tashkent city, Republic of Uzbekistan
e-mail: muhha8800@mail.ru
https://doi.org/10.5281/zenodo.17343047
Relevance:
In the current era of modern technologies, the supply of medicines is an important
issue in the treatment of diseases caused by heredity, gene mutations, cell membrane disorders of
higher organisms, climate degradation, and other causes, including in the situation caused by the
coronavirus. One of the complications remaining after the COVID-19 disease, which has spread
throughout the world, is the state of platelet aggregation. This naturally increases the demand for the
production of blood thinners (for example, heparin, cleacsan, etc.). In the context of the current
pandemic, one of the urgent problems is the search for cost-effective substitutes for such drugs using
the anticoagulant properties of endophytic fungi, their screening and recommendation for
pharmaceuticals and medicine.
Purpose of the research:
To study the diversity of endophytic fungi that produce anticoagulant
properties of medicinal plants of Uzbekistan.
Methods and techniques:
About 1300 plant species are found in the territory of the Chatkal
Biosphere Reserve of Uzbekistan, of which 200 species are medicinal plants. Medicinal plants are
the main source of endophytic fungi, which possess various antioxidant, anticancerogenic, including
anticoagulant properties.
In accordance with the goal, for the study of biological activity, endophytic fungi were isolated
from plants growing in the Chatkal Biosphere Reserve, which have been used in medicine since
ancient times in the treatment of cardiovascular diseases, diabetes mellitus, skin diseases, joint and
gastrointestinal diseases among various peoples, such as medicinal lemon balm (Melissa officinalis),
great chamomile (Chelidonium majus), bird cherry (Polygonum aviculare), tripartite thistle (Bidens
tripartite), fragrant basil (Ocimum basilicum), mint (Mentha piperita), saffron (Crocus sativus), and
climbing tamarisk (Thymus serpyllum).
Results: More than 55 samples of endophytic fungi were isolated from the leaves, stems, and
roots of the selected plants. Based on morpho-cultural characteristics, the isolated samples can be
classified into the following 10 genera: Acremonium, Alternaria, Aspergillus, Cuningamella,
Fusarium, Gliocladium, Mycelia, Mucor, Penicillium, Trichoderma. All strains have been placed in
the collection of the Institute of Microbiology of the Academy of Sciences of Uzbekistan for their
intended use.
When studying the anticoagulant properties of metabolites prepared and extracted from the
studied endophytic fungi (prothrombin time in blood plasma according to the Quick method), high
anticoagulant activity (blood clotting time 300 seconds) was detected in 29 strains.
Conclusions:
In conclusion, it can be said that the anticoagulant activity detected in endophytic
fungi isolated from medicinal plants was the main step in the work on their use as a pharmaceutical
raw material.
