502
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
STUDY OF THE ANTI-INFLAMMATORY ACTIVITY OF THE
SUPRAMOLECULAR COMPLEX OF GLYCYRRHIZIC ACID AND RUTIN
Baratov Q.R.
Rakhmonova G.G.
Tagayaliyeva N.A.
Yakubova R.A.
Institute of Bioorganic Chemistry, Academy of Sciences of the Republic of Uzbekistan,
Tashkent city, Republic of Uzbekistan
https://doi.org/10.5281/zenodo.17343395
In leading pharmaceutical companies, the development of new herbal medicines and their
application in medical practice has significantly increased. Medicinal plants are of great importance
in the global healthcare system due to the effective therapeutic properties of their biologically active
components. Currently, phenolic compounds isolated from plants show antioxidant, antihypoxic,
antiviral, anti-inflammatory, and vasodilatory activities. Among the most studied phenolic
compounds are the flavonoids rutin and quercetin. The main mechanism of flavonoid action is their
high antioxidant capacity, which provides a wide range of biological effects. However, due to their
low solubility, the study of their biological activity is limited. At present, many research centers
worldwide are conducting studies aimed at increasing the solubility of drugs (nanopharmacology).
To this end, approaches such as nanoparticles, nanocapsules, nanoemulsions, gelatin-based
formulations, and complexes with cyclodextrins and phospholipids have been proposed. In this
regard, supramolecular complexes also play an important role.
The experiments were performed on a model of acute exudative inflammation induced by
carrageenan (a classical phlogogen) via subplantar injection. The study was conducted on male rats
weighing 180 ± 20 g. Aqueous solutions of rutin and glycyrrhizic acid/rutin (GA/Rutin) complexes
were administered intragastrically at doses of 20–40 mg/kg. One hour later, 0.1 ml of a 1%
carrageenan solution was injected into the aponeurosis of the hind paw. Before carrageenan injection,
the initial paw volumes were measured. The anti-inflammatory effect was assessed 3 and 24 hours
after inflammation induction. Edema was determined as the difference between inflamed and non-
inflamed paw volumes. The anti-exudative effect (AEE) was calculated as the reduction of edema
compared to control animals.
In the carrageenan-induced inflammation model, maximal edema in control rats was observed
3 hours after injection, when paw volume increased by 93.8 ± 4.6% compared to baseline, and
decreased to 50.0 ± 6.5% after 24 hours. Similar dynamics were observed in the rutin- and GA/Rutin-
treated groups, but with significantly reduced edema. Rutin at doses of 20 and 40 mg/kg reduced paw
swelling 3 hours after carrageenan injection to 65.1 ± 17.0% and 53.3 ± 14.3% (p<0.05), and after 24
hours to 17.4 ± 3.75% and 22.4 ± 2.47% (p<0.01), respectively. The calculated AEE values for rutin
were 31.0% and 65.2% (20 mg/kg) and 43.0% and 55.2% (40 mg/kg) at 3 and 24 hours, respectively.
In the GA/Rutin-treated groups, paw edema at doses of 20 and 40 mg/kg was 44.1 ± 6.8% and 26.3
± 4.07% after 3 hours, and 12.8 ± 2.75% and 20.5 ± 6.82% after 24 hours, respectively, all
significantly lower compared to controls. The AEE values for GA/Rutin were 53.0% and 74.4% (20
mg/kg) and 72.0% and 59.0% (40 mg/kg) at 3 and 24 hours, respectively.
The obtained results show that under inflammatory conditions, the GA/Rutin supramolecular
complex demonstrates stronger anti-exudative activity compared to rutin alone at the studied doses
(20–40 mg/kg)
