DOI:
https://doi.org/10.37547/tajas/Volume03Issue03-08Keywords:
NMDA-receptors synaptosomes intracellular Ca2 , glutamateAbstract
The manuscript shows a short data used using fluorescent probes to study the effect of polyphenol PС-7 on changes in the dynamics of intracellular Ca2+ content in rat brain synaptosomes, depending on the site of glutamate binding on calcium channels by a specific mediator with glutamate. To measure the amount of cytosolic Ca2+ synaptosomes, we calculated using the Grinkevich equation. It has been shown that polyphenol PС-7 binds to the β1-subunit of the voltage-gated calcium channel and allosterically changes its conformation so that the conductivity for Ca2+ ions increases through the channel, the blocking effect of polyphenol PС-7 can be explained by its binding to voltage-gated calcium channels and activating them.
References
Siegel G.J., Albers R.W., Brady, S.T., Price, D.L. Basic Neurochemistry // 7th Ed. Academic Press. - 2006. - 992 p.
Magazannik LG The structure and functions of central and neuromuscular synapses // from the book. The modern course of classical physiology by Ed. Yu.V. Natochina and VA Tkachukayu - M.: Geotar-Media, 2007. P.59-99.
Olanow C.W., Stern M.B., Sethi K. The scientific and clinical basis for the treatment of Parkinson’s disease // Neurology. — 2009. — V. 72, 21, Suppl. 4. — 149 p.
Song, A., Charles, A., Koay, S. et al. Volumetric two-photon imaging of neurons using stereoscopy (vTwINS). Nat Methods 14, 420–426 (2017). https://doi.org/10.1038/nmeth.4226
Siegfried Weisenburger and Alipasha Vaziri. A Guide to Emerging Technologies for Large-Scale and Whole-Brain Optical Imaging of Neuronal Activity. Annual Review of Neuroscience. 2018. 41:1, 431-452
Velasco M, Rojas-Quintero J, Chávez-Castillo M, Rojas M, Bautista J, et al. (2017) Excitotoxicity: An Organized Crime at The Cellular Level. J Neurol Neurosci. Vol. 8 No. 3: 193
Auer RN (1986) Progress review: Hypoglycemic brain damage. Stroke 17: 699-708.
Vincent P, Mulle C (2009) Kainate receptors in epilepsy and excitotoxicity. Neuroscience 158: 309-323.
Fujikawa DG (2005) Prolonged seizures and cellular injury: understanding the connection. Epilepsy Behav 3: S3-S11.
Ward RJ, Lallemand F, De Witte P (2009) Biochemical and
neurotransmitter changes implicated in alcohol-induced brain damage in chronic or 'binge drinking' alcohol abuse. Alcohol Alcohol 44: 128-35.
Cotter DR, Pariante CM, Everall IP (2001) Glial cell abnormalities in major psychiatric disorders: The evidence and implications. Brain Res Bull 55: 585-595.
Olney JW (2003) Excitotoxicity, apoptosis and neuropsychiatric disorders. Curr Opin Pharmacol 3: 101-109.
Leitch B (1992) Ultrastructure of electrical synapses: review. Electron Microsc Rev 5: 311-339.
Dani A, Huang B, Bergan J, Dulac C, Zhuang X (2010) Super-resolution imaging of chemical synapses in the brain. Neuron 68: 843-856.
Blakely R, Edwards R (2012) Vesicular and plasma membrane transporters for neurotransmitters. Cold Spring Harb Perspect Biol 4: a005595.
Ramakrishnan NA, Drescher MJ, Drescher DG (2012) The SNARE complex in neuronal and sensory cells. Mol Cell Neurosci 50: 58-69.
Kew JN, Kemp JA (2005) Ionotropic and metabotropic glutamate receptor structure and pharmacology. Pyschopharmacology (Berl). 179 : 4-29.
Palmer LM, Shai AS, Reeve JE, Anderson HL, Paulsen O, et al. (2004) NMDA spikes enhance action potential generation during sensory input. Nat Neurosci 17: 383-90.
Moriyama Y, Yamamoto A (2004) Glutamatergic chemical transmission: Look! here, there, and anywhere. J Biochem 135: 155-163.
Berridge MJ (1997) Elementar y and global aspects of calcium signalling. J Physiol 499:291–306
Bootman MD, Collins TJ, Peppiatt CM et al (2001) Calcium signalling-an overview. Semin Cell Dev Biol 12:3–10
Bucurenciu I, Bischofberger J, Jonas P (2010) A small number of open Ca2+ channels trigger transmitter release at a central GABAergic syn-apse. Nat Neurosci 13:19–21
Khushwant S. Bhullar, H. P. Vasantha Rupasinghe, "Polyphenols: Multipotent Therapeutic Agents in Neurodegenerative Diseases", Oxidative Medicine and Cellular Longevity, vol. 2013, Article ID 891748, 18 pages, 2013. https://doi.org/10.1155/2013/891748
Torre, E. Molecular signaling mechanisms behind polyphenol-induced bone anabolism. Phytochem Rev 16, 1183–1226 (2017). https://doi.org/10.1007/s11101-017-9529-x
Khoshimov N.N., Nasirov K.E. Action of Cytisinum on the Transport Mediators and Calcium Channel of Glutamatergic Neurotransmitter Systems of the NMDA Receptor. European Journal of Medicine. 2017, 5(2): 56-63. DO- 10.13187/ejm.2017.2.31
Vauzour D. Dietary polyphenols as modulators of brain functions: biological actions and molecular mechanisms underpinning their beneficial effects. Oxid Med Cell Longev. 2012;2012:914273. doi:10.1155/2012/914273
Khoshimov N.N; Raimova G.M.; Nasirov K.E.; Rakhimov R.N.; Azizov V.G. "The Effect of Sp-6 On The Transport of Mediators of NMDA-Receptors and Ca2+-channels in Synaptosomes of rat brain. ”European Journal of Molecular & Clinical Medicine”, 7, 3, 2020, 2435-2446.
Campos-Esparza MR, Sánchez-Gómez MV, Matute C. Molecular mechanisms of neuroprotection by two natural antioxidant polyphenols. Cell Calcium. 2009 Apr;45(4):358-68. doi: 10.1016/j.ceca.2008.12.007. Epub 2009 Feb 7. PMID: 19201465.
Khoshimov N.N., Rakhimov R.N., Akhmedova G.B., Azizov V.G. Investigation of the effect of polyphenol euphorbin on the transport of L Glutamate and calcium channels to synaptosomes of rat brain // European Journal of Medicine. – 2018. – №. 6. – С. 72-82. DOI: 10.13187/ejm.2018.2.72
"European Convention for the Protection of Vertebrate Animals used for Experimental and other Scientific Purposes." Strasbourg. 1986. Available from: http://conventions.coe.int
Weiler, M. H., Gundersen C. B. and Jenden D. J. (1981) Choline uptake and acetylcholine synthesis in synaptosomes: Investigations using two differently labelled variants of choline//Neurochem. 36. P.1802-1812.
Grynkiewicz G., Poenie M., Tsien R.Y. 'A new generation of Ca2+, indicators with greatly improved fluorescence properties' J. Biol. Chem. Vol. 260, 1985, P. 3440 – 3450.
