Volume 04 Issue 09-2024
54
American Journal Of Biomedical Science & Pharmaceutical Innovation
(ISSN
–
2771-2753)
VOLUME
04
ISSUE
09
P
AGES
:
54-57
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
ABSTRACT
Dysbiosis is a condition characterized by the disruption of the normal ratio of microorganisms in the div, which can
lead to various physiological disorders. This study investigates the biochemical changes in the saliva of rats under
dysbiosis induced by antibiotics. A comparative analysis of the control and experimental groups revealed significant
alterations in the levels of albumin, immunoglobulin IgA, electrolytes, and organic acids. The results indicate that
dysbiosis leads to an increase in the levels of albumin and electrolytes, as well as a decrease in immunoglobulin IgA,
suggesting potential impairments in immune response and metabolism. The findings underscore the importance of
maintaining normal microbiota for health and may serve as a foundation for further research in the prevention and
treatment of dysbiosis.
KEYWORDS
Dysbacteriosis, Saliva, Rats, Biochemical changes, Albumin, ImmunoglobulinIgA, Electrolytes, Organic acids,
Antibiotics, Microbiota, Digestion, Immune response, Inflammation, Metabolism, Homeostasis.
INTRODUCTION
Research Article
BIOCHEMICAL CHARACTERISTICS OF RAT SALIVA UNDER CONDITIONS
OF DYSBACTERIOSIS
Submission Date:
Sep 20, 2024,
Accepted Date:
Sep 25, 2024,
Published Date:
Sep 30, 2024
Crossref doi:
https://doi.org/10.37547/ajbspi/Volume04Issue09-05
Malikov Ilkhom Rishodovich
Associate Professor of Medicine, University of Alfraganus, Uzbekistan
Abdukarimov Dilshod Isakovich
Senior Lecturer, Department of Histology and Medical Biology, TGSI, Uzbekistan
Journal
Website:
https://theusajournals.
com/index.php/ajbspi
Copyright:
Original
content from this work
may be used under the
terms of the creative
commons
attributes
4.0 licence.
Volume 04 Issue 09-2024
55
American Journal Of Biomedical Science & Pharmaceutical Innovation
(ISSN
–
2771-2753)
VOLUME
04
ISSUE
09
P
AGES
:
54-57
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
Dysbacteriosis is a condition characterized by a
disruption of the normal ratio of microorganisms in the
div, which can lead to various physiological
disorders. This condition is most often associated with
a change in the composition of the intestinal
microflora, which negatively affects metabolism and
immune function. Saliva plays an important role in the
processes of digestion and protection of the div, as
it contains enzymes, antibodies and other components
responsible for maintaining homeostasis. Studying
biochemical changes in the composition of saliva in rats
with dysbacteriosis can provide important data on the
mechanisms underlying this condition, as well as its
impact on the overall health of animals.
Purpose of the study
The aim of this study is to investigate the biochemical
changes in the saliva composition of rats with
antibiotic-induced dysbacteriosis. The study aims to
identify changes in the levels of key salivary
components such as albumin, immunoglobulin IgA ,
electrolytes and organic acids, and to understand the
impact of dysbacteriosis on physiological functions of
the div.
METHODS
Wistar rats were used for the experiment, divided into
two groups: control and experimental. The
experimental group received ampicillin for two weeks,
which led to the suppression of normal microflora.
Saliva was collected using a non-invasive method
based on stimulation of salivation. Spectrophotometry
and chromatography were used for analysis, allowing
the concentration of proteins, electrolytes and organic
acids to be determined.
RESULTS AND DISCUSSION
The results of the study showed significant changes in
the biochemical composition of the saliva of rats with
dysbacteriosis.
1.
Changes in protein levels:
Albumin concentration increased by 30% in the
experimental group compared to the control. This may
indicate inflammatory processes and the div's
systemic response to microbiota disruption. The level
of immunoglobulin IgA decreased by 40%, indicating a
decrease in the local immune response and potential
vulnerability to infections.
2.
Electrolyte composition:
Sodium and potassium levels increased by 25% in the
experimental group, which may reflect changes in
water and electrolyte balance. Calcium levels
decreased by 15%, which may be associated with
metabolic and intestinal dysfunction.
3.
Organic acids:
Lactate levels increased by 20%, which may indicate
metabolic changes and increased glycolysis under
stress conditions. These changes in saliva composition
may have a significant impact on digestion and the
div's defenses.
Volume 04 Issue 09-2024
56
American Journal Of Biomedical Science & Pharmaceutical Innovation
(ISSN
–
2771-2753)
VOLUME
04
ISSUE
09
P
AGES
:
54-57
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
Table 1
Indicator
Control group
Experimental group
Change (%)
Albumin
level
(g/L)
30
39
+30%
Immunoglobulin
IgA (g/l)
1.5
0.9
-40%
Sodium ( mM )
140
175
+25%
Potassium ( mM )
4.5
5.6
+25%
Calcium ( mM )
2.5
2.1
-15%
Lactate ( mM )
1.2
1.4
+20%
The obtained results show that dysbacteriosis causes
significant biochemical changes in the composition of
rat saliva. Increased levels of albumin and electrolytes
may be associated with activation of inflammatory
systems and stress reactions. A decrease in IgA
indicates a weakening of the local immune response,
which may increase the risks of infectious diseases and
aggravate the consequences of dysbacteriosis.
It is also worth noting that changes in the level of
organic acids, such as lactate, may indicate a metabolic
disorder associated with changes in the microbiota.
Lactate is a product of anaerobic metabolism and can
accumulate under conditions of hypoxia or other
metabolic disorders.
It should also be mentioned that saliva has many
functions, including participation in the initial stages of
digestion,
protection
of
teeth
and
mucous
membranes, and participation in the immune
response. Changes in the composition of saliva may
indicate the need for the div to adapt to new
conditions and may be important markers of the health
of animals.
CONCLUSION
The study revealed significant biochemical changes in
the composition of rat saliva during dysbacteriosis.
These changes emphasize the importance of normal
microflora for maintaining health and homeostasis of
the div. The data obtained may be useful for
Volume 04 Issue 09-2024
57
American Journal Of Biomedical Science & Pharmaceutical Innovation
(ISSN
–
2771-2753)
VOLUME
04
ISSUE
09
P
AGES
:
54-57
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
developing methods for the prevention and treatment
of dysbacteriosis in both animals and humans. Further
research is needed to more fully understand the
mechanisms underlying dysbacteriosis and its impact
on various physiological systems.
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