Volume 04 Issue 12-2024
18
American Journal Of Biomedical Science & Pharmaceutical Innovation
(ISSN
–
2771-2753)
VOLUME
04
ISSUE
12
P
AGES
:
18-21
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
ABSTRACT
This article investigates the biochemical characteristics of guinea pig saliva under conditions of dysbiosis. Dysbiosis,
which represents a disruption of normal microbiota, can significantly impact the health of animals and their
physiological processes. In the course of the experiment, key components of saliva, including proteins, carbohydrates,
and electrolytes, were analyzed to identify changes associated with microbiota imbalance. The results showed
significant deviations in the biochemical composition of guinea pig saliva, indicating potential mechanisms by which
dysbiosis affects digestion and immune response. These findings emphasize the importance of monitoring microbiota
status and biochemical indicators of saliva for maintaining the health of guinea pigs.
KEYWORDS
Dysbacteriosis, guinea pigs, saliva, biochemical characteristics, microflora, animal health, physiological processes.
INTRODUCTION
Research Article
CHANGES IN THE COMPOSITION OF GUINEA PIG SALIVA DURING
MICROBIOTA IMBALANCE
Submission Date:
December 01, 2024,
Accepted Date:
December 06, 2024,
Published Date:
December 11, 2024
Crossref doi:
https://doi.org/10.37547/ajbspi/Volume04Issue12-04
Babakulov Sharof Khamrokulovich
Associate Professor of the Department of Surgery and Military Field Surgery, TGSI, Uzbekistan
Babakulova Shakhlo Khamidullaevna
Assistant of the Department of Public Health, Healthcare Management and Physical Culture of TGSI,
Uzbekistan
Abdukarimov Dilshod Isakovich
Senior Lecturer, Department of Histology and Medical Biology, TGSI, Uzbekistan
Journal
Website:
https://theusajournals.
com/index.php/ajbspi
Copyright:
Original
content from this work
may be used under the
terms of the creative
commons
attributes
4.0 licence.
Volume 04 Issue 12-2024
19
American Journal Of Biomedical Science & Pharmaceutical Innovation
(ISSN
–
2771-2753)
VOLUME
04
ISSUE
12
P
AGES
:
18-21
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
Dysbacteriosis is a condition characterized by a
disruption of the normal balance of microbiota in the
div. This phenomenon can occur under the influence
of various factors, such as poor nutrition, stress,
infectious diseases or the use of antibiotics. In guinea
pigs, as in other animals, dysbacteriosis can lead to
serious disturbances in the digestive system and
weakened immune defense.
Saliva plays a key role in the digestive process and in
protecting the mucous membranes of the oral cavity.
It contains many biochemical components, including
enzymes, proteins and antibodies, which help maintain
the health of the div. Studying changes in the
biochemical composition of saliva in dysbacteriosis can
provide valuable information about the health of
guinea pigs and the mechanisms that contribute to the
development of diseases.
Purpose of the study
The aim of this study is to analyze the biochemical
characteristics of guinea pig saliva under conditions of
dysbacteriosis, to identify the main changes and their
potential impact on animal health.
METHODS
The study involved 30 guinea pigs, aged 8-12 weeks.
The animals were divided into two groups: a control
group (15 guinea pigs, without signs of dysbacteriosis)
and an experimental group (15 guinea pigs, in which
signs of dysbacteriosis were established). Guinea pigs
were kept in standard conditions, with access to clean
water and balanced feed. All animals were vaccinated
and clinically examined before the experiment.
Saliva was collected by encouraging animals to chew
soft food for 15 minutes. The collected material was
placed in sterile containers and stored at -20°C until
analysis.
Analysis of biochemical characteristics of saliva was
carried out using the following methods:
•
Determination of protein levels: Biuret test
method.
•
Measurement of carbohydrate content:
Phenolic test method.
•
Electrolyte analysis: Ion chromatography.
•
Measurement
of
lysozyme
activity:
Colorimetric method.
The obtained data were processed using a statistical
software package. Differences between groups were
analyzed using a t-test, with a significance level of p <
0.05.
RESULTS AND DISCUSSION
The study revealed significant changes in the
biochemical composition of guinea pig saliva under
conditions of dysbacteriosis.
1.
Protein level
o
In the experimental group, the protein level in
saliva averaged 2.5 g/ dL , which was significantly
higher than in the control group (1.8 g/ dL ) (p < 0.05 ).
2.
Carbohydrate content
o
The carbohydrate content in the saliva of
guinea pigs with dysbacteriosis increased to 0.6 g/ dl ,
Volume 04 Issue 12-2024
20
American Journal Of Biomedical Science & Pharmaceutical Innovation
(ISSN
–
2771-2753)
VOLUME
04
ISSUE
12
P
AGES
:
18-21
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
compared with 0.4 g/ dl in the control group (p < 0.05
).
3.
Electrolyte concentration
o
Salivary sodium and potassium levels also
showed significant differences: sodium was 140 mM /L
in the experimental group versus 120 mM /L in the
control group (p < 0.01 ), while potassium levels
increased from 4.0 mM /L to 5.2 mM /L (p < 0.05).
4.
Lysozyme activity
o
The activity of lysozyme in the saliva of guinea
pigs with dysbacteriosis was 0.25 μg/ml, which is lower
than in the control group, where this indicator was 0.35
μg/ml (p < 0.05 ).
All the changes identified were statistically significant,
confirming the impact of dysbacteriosis on the
biochemical characteristics of guinea pig saliva. The
results highlight the importance of saliva as an
indicator of animal health and the potential impact of
dysbacteriosis on their physiological functions.
CONCLUSION
A study of the biochemical characteristics of guinea pig
saliva under conditions of dysbacteriosis showed
significant changes in the composition of saliva
compared to the control group. An increase in protein
and carbohydrate levels, as well as a change in the
concentration of electrolytes, indicate disturbances in
physiological processes in the animals' bodies. A
decrease in lysozyme activity indicates a possible
weakening of the immune defense.
The obtained data highlight the importance of
monitoring the microflora status and biochemical
parameters of saliva for the early detection of
dysbacteriosis and other diseases. This may help in
developing effective prevention and treatment
strategies aimed at maintaining the health of guinea
pigs.
Further research is needed to better understand the
mechanisms underlying changes in the biochemical
composition of saliva and to assess the impact of
various factors on the health of guinea pigs.
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American Journal Of Biomedical Science & Pharmaceutical Innovation
(ISSN
–
2771-2753)
VOLUME
04
ISSUE
12
P
AGES
:
18-21
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
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