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MORPHOLOGY OF LOWER LIMB MUSCLES IN MECHANICAL
INJURY OF LOWER LIMB
Umurov Bobirjon Fayzilloevich
Bukhara State Medical Institute
https://doi.org/10.5281/zenodo.13960282
Summary:
The study included 48 adult rats with mechanical injuries of
the lower limb, divided into two groups. The first group (24 rats) received local
anesthesia, the second group (24 rats) - general anesthesia. In all patients,
biopsies were taken from the muscles of the lower limb for histological analysis
24 and 72 hours after injury. The following morphometric parameters were
assessed: muscle fiber edema, inflammatory infiltration, degenerative changes
and muscle tissue regeneration.
Keywords:
morphometry, edema, degenerative changes, muscle tissue
regeneration
Research aim.
The aim of the study was to compare morphometric
changes in lower limb muscles after mechanical injury in rats receiving local and
general anesthesia.
Мaterials and methods.
The experiment was conducted on 48 sexually
mature rats, 5-6 months old (m=200-220 g). The animals were kept in standard
conditions that meet sanitary regulations. Special marks on the div were used
to identify the rats. During the experiment, the animals were healthy, with no
changes in behavior. The rats in the experiment were anesthetized by
intraperitoneal administration of bupivicaine (150 mg/kg). In two groups, on
the 1st and 3rd days after, the muscles of the lower limb were taken during
autopsy in compliance with the principles of humane treatment of animals, some
of the animals were removed from the experiment by euthanasia, under
chloroform anesthesia, by puncture of the left ventricle until complete
exsanguination. The obtained biomaterial (muscle tissue) was fixed in 10%
formalin solution. After fixation, the muscle tissue was excised and embedded in
paraffin using the standard technique. Next, sections 5-7 µm thick were made
and stained with hematoxylin and eosin.
Results and discussion.
These data allow a visual comparison of the degree of
muscle tissue damage and the inflammatory response depending on the type of
anesthesia in the two groups over time. Average area of necrotic fibers (mm²):
reflects the degree of muscle tissue damage. In the general anesthesia group,
there is a larger area of necrotic fibers, which indicates more serious damage.
The second indicator indicates the intensity of the inflammatory response. In the
general anesthesia group, the percentage of inflammatory cells is higher, which
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indicates a more active inflammatory reaction. The next indicator is the state of
the blood supply to the muscle tissue. In the local anesthesia group, the density
of the vascular network is higher, which may indicate better blood supply. The
last morphometric parameter measures the volume of edema in the muscle
tissue. In the general anesthesia group, the level of edema is higher, which
indicates more pronounced tissue damage. Based on the data obtained, the
results were entered in тables No. -1 and 2.
Таблица №1.
Morphometric data 24 hours after injury
Parameter
Group I (local
anesthesia)
Group II (general
anesthesia)Group II I
(local anesthesia)
Average area of
necrotic fibers (mm²)
0,15 ± 0,02
0,30 ± 0,05
Percentage of
inflammatory cells (%)
20 ± 3
35 ± 5
Vascular density
(vessels/mm²)
8 ± 1
6 ± 1
Edema level (mm³)
1,2 ± 0,2
2,5 ± 0,3
Таблица №2.
Morphometric data 72 hours after injury
Parameter
Group I (local
anesthesia)
Group II (general
anesthesia)
Средняя площадь
некротизированных
волокон (мм²)
0,10 ± 0,01
0,25 ± 0,04
Percentage of
inflammatory cells (%)
15 ± 2
30 ± 4
Vascular density
(vessels/mm²)
10 ± 1
7 ± 1
Edema level (mm³)
0,8 ± 0,1
2,0 ± 0,2
CONCLUSION.
Thus, the analysis of these indicators allows us to conclude that
general anesthesia leads to more serious damage to muscle tissue and a more
pronounced inflammatory reaction than local anesthesia.
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