INVESTIGATING THE IMPACT OF SUCROSE ON MICROBIAL PECTIN ESTERASE AND PECTATE LYASE ACTIVITY
Keywords:
Abstract
Pectin esterases and pectate lyases are key enzymes involved in the degradation of pectin, a complex polysaccharide found in plant cell walls. This study explores the influence of sucrose, a common sugar, on the activity of microbial pectin esterases and pectate lyases. Enzyme assays were conducted using pectin as a substrate in the presence of varying concentrations of sucrose. The results indicated that sucrose exerted distinct effects on these enzymes, with some concentrations enhancing activity while others displayed inhibitory effects. The intricate interplay between sucrose concentration and enzyme activity provides insights into the regulation of pectin degradation pathways and underscores the complexity of microbial enzyme interactions.
Similar Articles
-
CULTURE IS A CERTAIN LEVEL OF HISTORICAL DEVELOPMENT OF SOCIETY, HUMAN CREATIVE POWER AND ABILITIES
-
INNOVATIVE APPROACH TO MUSIC LISTENING ACTIVITIES
European International Journal of Multidisciplinary Research and Management Studies: No. 03 (2023) / Baxriyev Axmad Raxmatovich, -
ISSUES AND SOLUTIONS OF MUSICAL EDUCATION AND ENSURING CONTINUITY OF MUSICAL EDUCATION IN THE PERFORMANCE OF THE INSTRUMENT
European International Journal of Multidisciplinary Research and Management Studies: No. 04 (2023) / Azizov Orifjon Ganiyevich, -
INTERPRETATION OF THE WORK OF RAKHIM AKHMEDOV IN THE ART OF PAINTING OF UZBEKISTAN
European International Journal of Multidisciplinary Research and Management Studies: No. 04 (2022) / Sharipov Javlon Sharifjon O'gli, -
ON THE STAGES OF DEVELOPMENT OF FOLK MUSIC CREATION AND THE ACTIVITY OF MUSIC ARTISTS
-
INFLUENCE OF MUSIC ON SPIRITUALITY
-
IMPROVING THE PEDAGOGICAL AND PSYCHOLOGICAL COMPETENCE OF FIRST-YEAR CHILDREN IN PRESCHOOL EDUCATION
European International Journal of Multidisciplinary Research and Management Studies: No. 05 (2022) / Mamatkulova Shakhnoza , -
THE PLACE OF MUSICAL EDUCATION IN THE EDUCATION OF AN ARTIST
European International Journal of Multidisciplinary Research and Management Studies: No. 10 (2023) / Radjabov Jamshid Hikmatovich, -
PHYSICAL EDUCATION IN HIGHER EDUCATION INSTITUTIONS
-
THE ROLE AND SIGNIFICANCE OF KHORAZM FOLK MUSIC IN THE FORMATION OF STUDENTS' MORAL CULTURE
European International Journal of Multidisciplinary Research and Management Studies: No. 01 (2023) / Rajabov Shakhzodbek Bakhrom O`G`Li,
References
Aneja KR (1996). Production of pectolytic enzymes. In: Experiments in Microbiology, Plant Pathology, Tissue Culture and Mushroom Cultivation. Wishwa Prakashan, New Agel International (P) Ltd., New Delhi, pp.195-197.
Archana A, Satyanarayana T (1997). Solid state fermentation for the production of Industrial enzymes. Current Science. 77: 149 - 162.
Bateman DF, Millar RL (2013). Pectic enzymes in tissue degrdataion. Annual Reviews in Phytopathology. 4: 118- 146.
Budiatmen S, Lonsane BK (2014). Cassava fibrous waste residue: a substitute to wheat bran in solid state fermentation. Biotechnology Letters. 9: 597 - 900.
Ceci L, Loranzo, J (1998). Determination of enzymatic activities of commercial pectinases for the clarification of apple juice. Food Chemistry. 61: 237 - 241.
Chatterjee AK, Buchanan GE, Behrens MK, Starr MP (2010). Synthesis and excretion of polygalacturonic acid transeliminase in Erwinia, Yersinia and Klebsiella species. Canadian Journal of Microbiology. 25: 94 –102.
Chesson A (2009). A review – Maceration in relation to the post harvest handling and processing of plant material. Journal of Applied Bacteriology. 48: 1 - 45.
Cole M, Wood RKS (2006). Pectic enzyme and phenolic substances in apple rotted by fungi. Annals of Botany. 25: 435-452.
Colmer A, Rein JL, Mount MS (2008). Pectic Enzymes – Assays. Methods in Enzymology. 16: 329 - 335.
Conway WS, Gross KC, Boyer CD, Sams CE (2008). Inhibition of Penicillium expansum polygalacturonase activity by increased apple cell wall. Phytopathology. 78: 1052 - 1055.
Elumalai RP, Mahadevan A (2005). Characterization of pectate lyase produced by Pseudomonas marginalis and cloning of pectate lyase genes. Physiology and Molecular Plant Pathology. 46: 109 - 111.
Fogarty WM, Kelly CT (2003). Pectic Enzymes. In: Fogarty, W.M. (ed.) Microbial Enzymes and Biotechnology Applied Science Publishers, London, pp. 131-182
Garzon CG, Hours RA (2002). Citrus waste: An alternative substrate for pectinase production in solid stage culture. Bioresource Technology. 39: 93 - 95.
Geetha M, Saranraj P, Magalakshmi S, Reetha D (2012). Screening of pectinase producing bacteria and fungi for its pectinolytic activity using fruit wastes. International Journal of Biochemistry and Biotech Sciences. 1: 30 -42.
