554
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
ANALYSIS OF TORASEMIDE BY CHROMATOSPECTROPHOTOMETRIC
METHOD
Abdullabekova N.A.
1
Usmanalieva Z.U.
2
1
Tashkent Pharmaceutical Institute, Tashkent city, Republic of Uzbekistan
2
Pharmaceutical Educational and Research Institute, Tashkent city, Republic of Uzbekistan
e-mail: nargizachemist@mail.ru
https://doi.org/10.5281/zenodo.17344243
Relevance
: Torasemide is a loop diuretic. When used in low doses, its diuresis rate is similar
to that of the thiazide group, and the maximum effect is achieved 2 hours after administration. The
drug is rapidly and almost completely absorbed into the blood. In patients with impaired liver
function, an increase in the concentration of torasemide in the blood plasma is observed. Therefore,
such patients should exercise caution. The toxic effect of torasemide can lead to serious complications
such as arterial hypotension, confusion, thrombosis, cardiac arrhythmias, angina pectoris and acute
myocardial infarction as a result of impaired water and electrolyte balance, increased metabolic
alkalosis and excessive diuresis. From the urinary system. In patients with impaired urinary excretion,
it can lead to rapid urine output, its retention and overdistension of the bladder. Sometimes the level
of uric acid, glucose, lipids in the blood increases.
The purpose of the study:
In forensic chemistry practice, the chromatospectrophotometric
method is used to determine the amount of toxic substances extracted from biological objects and
biological fluids. In this case, toxic substances extracted from biological objects are purified from
foreign substances by thin layer chromatography, and then the amount of the substance is determined
by UV-spectrophotometry. Therefore, the aim of the study was to determine the amount of the drug
torasemide by chromatospectrophotometric method.
Methods and styles:
To determine the amount of torasemide by chromatospectrophotometric
method, 0.1 ml of a standard solution of torasemide in ethyl alcohol containing 1 mg/ml was dripped
onto the start line of 5 chromatographic plates of the brand "Silufol UV 254" and dried at room
temperature. Then, the plates were placed in chromatographic chambers saturated with a mixture of
organic solvents: ethyl acetate: ethanol: ammonia 25% (8.5:1:0.5) and their vapors were added. The
solvent mixture rose to a height of 10 cm and when it reached the finish line, the plates were removed
and dried at room temperature. In order to determine the areas of elevated concentration of substances
on the chromatographic plates, they were marked using a UB-254 lamp, and the part where the drug
substances were dropped was scraped off the sorbent layers. The eluate was filtered into a small
amount of 0.1M hydrochloric acid, transferred to a 10 ml volumetric flask and made up to the mark
with 0.1M hydrochloric acid. The solution was thoroughly mixed and filtered through a 0.45 micron
filter. Then, the analysis was carried out on an Agilent Technologies 8453 E Spectroscopy System
UV spectrophotometer, in a cuvette with a layer thickness of 10 mm, at a wavelength of 287 nm.
0.1M hydrochloric acid was taken as a reference solution. The amount of torasemide was determined
spectrophotometrically using a calibration chart prepared based on standard sample solutions of
torasemide in 0.1M hydrochloric acid, which contained 0.2-2.0 μg/ml in a pre-prepared solution. The
metrological report of the quantitative analysis of torasemide determined by the
chromospectrophotometric method was calculated according to the DF XI edition.
Results:
The chromatospectrophotometric analysis of torasemide revealed an average
determination of 90.78%, with a relative error of 2.17%.
555
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
Conclusions:
The amount of torasemide was determined by chromatospectrophotometric
method. The developed method can be used to purify and quantify torasemide isolated from biological
objects.
