421
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
QUANTITATIVE DETERMINATION OF ASCORBIC ACID IN A DIETARY
SUPPLEMENT VITAMINNI BY HPLC METHOD
Tleubaeva N.J.
Khamdamov M.M.
Tashkent Pharmaceutical Institute,
e-mail: nargizatleubaeva.2508@gmail.com
tel: (91) 302-07-27
https://doi.org/10.5281/zenodo.17341411
Relevance:
Gummy dietary supplements in the form of jelly bears represent a modern and
convenient dosage form of vitamin-mineral complexes. One of the most demanded components of
such supplements is ascorbic acid (vitamin C), which possesses pronounced antioxidant properties
and is involved in numerous metabolic processes of the human div. To control the quality of such
dietary supplements, it is necessary to use highly accurate analytical methods, among which the most
sensitive is the high-performance liquid chromatography (HPLC) method.
Aim of the study:
Quantitative determination of ascorbic acid in the dietary supplement
VitaMinni (gummy bears) using HPLC.
Methodology:
• Chromatographic conditions: HPLC system equipped with a UV detector; C18 column
(150×4.6 mm, 5 µm); mobile phase – phosphate buffer solution with pH 3.0 : acetonitrile (60:40);
flow rate – 1.0 ml/min; injection volume – 20 µl; detection – UV at 265 nm.
• Standard solution: about 50 mg (accurately weighed) of standard ascorbic acid was placed
into a 50 ml volumetric flask, dissolved in 25 ml of water, and the volume was adjusted to the mark
with the same solvent (final concentration: 1.0 mg/ml).
• Test solution: about 3 g (accurately weighed) of VitaMinni gummy bears were finely ground
and quantitatively transferred into a 100 ml volumetric flask using 50 ml of water, dissolved with
ultrasonic assistance, the volume was made up to the mark with the same solvent, mixed, and filtered
through a 0.45 µm membrane filter.
Peak area of standard sample solution:
Peak area of the test solution:
422
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
Results:
The average weight of the gummy bears was 3.105 g. Authenticity: The retention time
of the main peak on the chromatogram of the test solution prepared for quantitative determination
corresponded to the retention time of the main peak in the chromatogram of the reference solution.
The content of ascorbic acid in one VitaMinni gummy was 10.4 mg. The quantitative determination
results are presented in the table below.
Table №1
Accurately weighed
samples taken, gr
Retention times, min
Peak areas of solutions
Standard samples
0,0501
0,950
9592307
0,0504
0,956
9631081
0,0502
0,952
9592685
Test samples
3,2962
0,948
1102056
3,1586
0,940
978908
3,2271
0,946
1039393
Conclusion:
the HPLC method provides reliable and accurate determination of ascorbic acid
content in the jelly forms of VitaMinni and can be recommended for the quality control of dietary
supplements in gummy formulations.
