518
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
ANTIPROLIFERATIVE ACTIVITY OF AQUEOUS-ETHANOL EXTRACTS OF
LENZITES BETULINUS
AND
TRAMETES VERSICOLOR
AGAINST BREAST
CARCINOMA CELLS
Harbatsevich H.I.¹
Doroshenko T.M.²
Portyanko A.S.²,
Bobrova N.M.²
Severin I.N.²
1
Belarusian State Medical University, Minsk, Republic of Belarus
2
N.N. Alexandrov National Cancer Centre of Belarus, Minsk, Republic of Belarus
e-mail: hleb.harbatsevich@gmail.com, tel. (71)-256-39-54
https://doi.org/10.5281/zenodo.17343775
Relevance.
Malignant tumors remain one of the leading causes of mortality worldwide, with
breast carcinoma ranking first in incidence among women. Despite the development of modern
therapeutic approaches, drug resistance and pronounced side effects of cytostatic drugs necessitate
the search for new selective agents with antiproliferative activity. A promising direction is the study
of biologically active compounds from natural sources, among which polypore fungi are of particular
interest due to their richness in polysaccharides and triterpenoids with pronounced
immunostimulatory and cytotoxic properties. In particular, the relatively unexplored yet widely
distributed species
Lenzites betulinus
and
Trametes versicolor
are potential sources of new anticancer
agents.
Aim of the study:
To investigate the antiproliferative activity of aqueous-ethanol extracts of
L.
betulinus
and
T. versicolor
against breast carcinoma ZR-75 cells.
Materials and methods:
Extracts were obtained by maceration of fungal material in 70%
ethanol. Human cell cultures were used in the experiment: Bj-hTERT (normal fibroblasts) and ZR-
75 (breast carcinoma). Doxorubicin (20 μM) served as the reference drug. A 1% stock solution of the
studied extracts was prepared in phosphate-buffered saline (pH 7.4) with 1% DMSO, followed by
filtration through a 0.25 μm filter. Cell viability was assessed using the MTT assay.
Results:
At low concentrations (1:20), the extracts exhibited the highest selectivity: the
L.
betulinus
extract inhibited ZR-75 cell growth by 63.9±7.1%, while the effect on normal Bj-hTERT
fibroblasts was significantly lower (18.6±20.4%, p<0.05). For
T. versicolor
, inhibition of ZR-75 cells
reached 69.0±3.2%, while only 30.7±13.9% inhibition was observed in Bj-hTERT cells (p<0.05). In
contrast, doxorubicin suppressed both tumor (72.9±5.2%) and normal cells (82.5±5.3%).
At higher concentrations (2:20–5:20), the effect of the extracts on ZR-75 cells remained
comparable to doxorubicin (up to 69.5±6.5% for
L. betulinus
and 68.2±4.6% for
T. versicolor
), but
the impact on Bj-hTERT cells significantly increased (up to 71.6±7.7% and 69.8±3.8%, respectively).
Conclusions:
Both studied extracts demonstrate antiproliferative effects against breast
carcinoma cells: at low concentrations, they show relative selectivity toward tumor cells, but with
increasing concentrations, their action becomes non-specific, though stronger (comparable to
doxorubicin). The obtained results confirm the prospects for further investigation of
Lenzites
betulinus
and
Trametes versicolor
as potential sources of new anticancer compounds.
