534
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
EFFECT OF POLYPHENOLS ISOLATED FROM EUPHORBIA FRANCHETII
(B.FEDTSCH) ON LPO PROCESSES IN MITOCHONDRIA
Isamuhamedova D.R.
1
Ergashev N. A.
2
Rakhimov R. N.
3
1
Regulation named after Uzbekistan National Pedagogy University,
Tashkent city, Republic of Uzbekistan;
2
National University of Uzbekistan Biophysics and biochemistry institute,
Tashkent city, Republic of Uzbekistan;
3
UzR FA Bioorganic chemistry institute Tashkent city, Republic of Uzbekistan.
dildoraisamuxamedova@gmail.com phone : (97) 754-40-58
https://doi.org/10.5281/zenodo.17344033
The production of free radicals in various tissues and cells of the div through many
endogenous and exogenous pathways causes oxidative stress, which leads to the development of
various diseases in humans. Therefore, the study of the antioxidant properties of biologically active
substances isolated from plants is of great importance. It is known that epidemiologically, fruits and
vegetables rich in antioxidants in the diet prevent the development of many diseases associated with
oxidative stress. Recent studies have shown that mitochondria play an important role in apoptotic and
necrotic cell death. As a result of mitochondrial dysfunction, the number of mitochondria in tissues
decreases, deep ultrastructural anomalies of organelles develop, mitochondrial biogenesis is
disrupted, the activity of enzyme complexes of the respiratory chain changes, and ATP synthesis is
inhibited. At the same time, mitochondrial dysfunction can lead to calcium homeostasis disruption,
excessive production of reactive oxygen species, as well as the transition of the megapore to an open
conformation, a decrease in the expression of the antiapoptotic protein Bcl-2, the release of
cytochrome c, the activation of proapoptotic caspase-3 and the DNA repair enzyme poly (-ADP-
ribose) polymerase. From the above, it can be seen that a specific mitochondrial dysfunction is
observed in the process of lipid peroxidation (LPO) that develops during oxidative stress.
Purpose of the study.
Therefore, the study investigated the effect of 1-O-galloyl-4,6-
hexahydroxydiphenoyl-
-D-glucose polyphenol isolated from
the Euphorbia franchetii plant
on the
LPO process in rat liver mitochondria.
Materials and methods of the study.
Rat liver mitochondria were isolated by differential
centrifugation. The composition of the separation medium: 250 mM sucrose, 10 mM Tris-HCl, 1 mM
EDTA, pH-7.4. IM : KCl - 125 mM, Tris-HCl - 10 mM, pH 7.4; The process of lipid peroxidation
(LPO) in mitochondria was induced in the presence of 10 μM FeSO
4
, 600 μM ascorbic acid. The
protein content in the cuvette was 0.3-0.4 mg/ml. The mitochondrial fluorescence was determined at
540 nm using a V-5000 spectrophotometer.
Results and their analysis. The change in the intensity
of the LPO process in mitochondria
under the influence of Fe
2+
/ascorbate was set as 100% control. 1-O-galloyl-4,6-
hexahydroxydiphenoyl-
-D-glucose to the incubation medium when polyphenol substance is added
in the amount of 1 μM , the LPO process is increased by 21±1.7%, in the amount of 2 μM by
39±1.31%, in the amount of 3 μM by 54±2.2%, in the amount of 4 μM by 74±1.91%, in the amount
of 5 μM It was found that it inhibited by 88±1.7%.
Conclusion.
The results show that 1-O-galloyl-4,6-hexahydroxydiphenoyl-
-D-glucose
polyphenol exhibited high antioxidant activity at the low micromolar concentrations studied. It
535
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
inhibited the Fe
2+
/ascorbate-induced LPO process in mitochondria and exhibited membrane-active
properties.
