Authors

  • Muzaffar Pulatov
    Andighan State Medical Institute

DOI:

https://doi.org/10.71337/inlibrary.uz.jmsi.109920

Abstract

Acute intestinal infections (AIIs) remain one of the most frequent causes of morbidity across various age groups, particularly among children and immunocompromised patients. Bacterial or mixed etiology infections often require antibiotic therapy, which can significantly disrupt the gut microbiota. This dysbiosis may manifest clinically and lead to prolonged gastrointestinal symptoms, malabsorption, and increased susceptibility to secondary infections. This article reviews current knowledge regarding the pathophysiology of dysbiosis induced by antibiotic treatment of AIIs and presents evidence-based strategies for restoring a balanced gut microbiota. Key approaches include using probiotics, prebiotics, and synbiotics, alongside antibiotic stewardship and careful monitoring of gastrointestinal function.


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TREATMENT OF DYSBIOSIS DURING ANTIBIOTIC THERAPY FOR ACUTE

INTESTINAL INFECTIONS

Pulatov Muzaffar Ergashevich

Assistants of the Department of Infectious Diseases

Andighan State Medical Institute

Abstract:

Acute intestinal infections (AIIs) remain one of the most frequent causes of morbidity

across various age groups, particularly among children and immunocompromised patients.

Bacterial or mixed etiology infections often require antibiotic therapy, which can significantly

disrupt the gut microbiota. This dysbiosis may manifest clinically and lead to prolonged

gastrointestinal symptoms, malabsorption, and increased susceptibility to secondary infections.

This article reviews current knowledge regarding the pathophysiology of dysbiosis induced by

antibiotic treatment of AIIs and presents evidence-based strategies for restoring a balanced gut

microbiota. Key approaches include using probiotics, prebiotics, and synbiotics, alongside

antibiotic stewardship and careful monitoring of gastrointestinal function.

Keywords

: acute intestinal infections, antibiotics, dysbiosis, probiotics, gut microbiota,

antibiotic stewardship

ЛЕЧЕНИЕ ДИСБАКТЕРИОЗА ВО ВРЕМЯ АНТИБИОТИКОТЕРАПИИ ОСТРЫХ

КИШЕЧНЫХ ИНФЕКЦИЙ

Пулатов Музаффар Эргашевич

Ассистент кафедры инфекционных болезней

Андиганский государственный медицинский институт

Аннотация:

Острые кишечные инфекции (ОКИ) остаются одной из наиболее частых

причин заболеваемости в различных возрастных группах, особенно среди детей и

пациентов с ослабленным иммунитетом. Бактериальные или смешанные инфекции часто

требуют антибактериальной терапии, которая может значительно нарушить микробиоту

кишечника. Этот дисбактериоз может проявляться клинически и приводить к длительным

желудочно-кишечным симптомам, мальабсорбции и повышенной восприимчивости к

вторичным инфекциям. В этой статье рассматриваются современные знания о

патофизиологии дисбактериоза, вызванного антибиотикотерапией ОКИ, и представлены

научно обоснованные стратегии восстановления сбалансированной микробиоты

кишечника. Ключевые подходы включают использование пробиотиков, пребиотиков и

синбиотиков, а также рациональное использование антибиотиков и тщательный

мониторинг функции желудочно-кишечного тракта.

Ключевые слова:

острые кишечные инфекции, антибиотики, дисбактериоз, пробиотики,

микробиота кишечника, рациональное использование антибиотиков

INTRODUCTION

Acute intestinal infections (AIIs) are among the most common causes of acute gastroenteritis

worldwide, leading to significant morbidity and, in certain regions, elevated mortality, especially


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in pediatric populations 111. Their etiological agents include bacteria (e.g.,

Salmonella spp.

,

Shigella spp.

, pathogenic

Escherichia coli

), viruses (rotavirus, norovirus), and protozoa [1].

Depending on the severity and cause, bacterial AIIs frequently necessitate antibiotic therapy.

While antibiotics can be lifesaving, they often disrupt the gut microbiome’s natural balance,

resulting in dysbiosis and associated complications 222.
The present article provides an overview of how antibiotic therapy contributes to dysbiosis

during AIIs, the clinical implications of this dysbiosis, and current evidence-based interventions

that aim to restore a healthy gut microbiota [2].
Etiology and pathogenesis of aii-related dysbiosis
Mechanisms of Antibiotic-Induced Dysbiosis - Antibiotics work by targeting bacterial pathogens,

but they also non-selectively impact commensal flora in the gut. Key mechanisms include:

Reduced Microbial Diversity: Broad-spectrum antibiotics can eradicate beneficial bacteria (e.g.,

Bifidobacterium

spp.,

Lactobacillus

spp.), diminishing overall richness and diversity of the

microbiome 333. Overgrowth of Resistant Strains: With commensals depleted, antibiotic-

resistant organisms (e.g.,

Clostridioides difficile

) can proliferate, leading to secondary infections

and inflammation. Metabolic Shifts: Loss of beneficial bacteria impairs short-chain fatty acid

(SCFA) production, influencing gut pH and epithelial integrity [3].
Clinical Manifestations - Prolonged Diarrhea: Antibiotic-associated diarrhea can be exacerbated

in an environment of ongoing intestinal infection. Malabsorption: Dysbiosis reduces the capacity

of the gut to digest and absorb nutrients. Recurrence or Persistence of Infection: An imbalanced

microbiota may fail to outcompete remaining pathogens [4]. Risk of Secondary Infections: The

prevalence of opportunistic pathogens (e.g.,

C. difficile

) increases under dysbiotic conditions.

DIAGNOSTIC CONSIDERATIONS

Microbiological Testing - Stool Culture: Useful for identifying primary pathogenic bacteria, as

well as any emerging resistant flora. Molecular Methods (PCR): Can detect low levels of

pathogenic bacteria or viruses, and sometimes quantify pathogenic or beneficial strains.
Microbiota Profiling - 16S rRNA Gene Sequencing: Provides insights into microbial community

composition. Although more common in research settings, next-generation sequencing

techniques are increasingly being used clinically to assess the extent of dysbiosis. Metabolomic

Analysis: Evaluates functional changes (e.g., SCFA production). Still largely in the research

phase but offers a deeper understanding of metabolic alterations [5].
Clinical markers - Inflammatory Markers: Elevated C-reactive protein (CRP) or fecal

calprotectin can indicate intestinal inflammation [6]. Symptom Assessment: Frequency of

diarrhea, abdominal pain, bloating, and presence of systemic symptoms help evaluate the

severity of dysbiosis.

TREATMENT STRATEGIES

Rational Antibiotic Therapy - Narrow-Spectrum Agents: Whenever possible, select agents that

specifically target the identified pathogen. Reducing the use of broad-spectrum antibiotics helps

preserve beneficial gut flora. Antibiotic Stewardship: Includes de-escalating therapy based on

pathogen sensitivities, limiting treatment duration, and avoiding inappropriate antibiotic use.
Probiotic Supplementation - Single-Strain Probiotics:

Lactobacillus rhamnosus GG

,

Saccharomyces boulardii

, and

Bifidobacterium

species are commonly used. Studies show they

can reduce the incidence and severity of antibiotic-associated diarrhea 444. Multi-Strain

Formulations: Combining different species may broaden the coverage and enhance resilience

against multiple pathogens [7]. Timing and Dosage: Probiotics are most effective when initiated

early in therapy and continued for at least 1–2 weeks post-antibiotic course. Dosages typically

range from 10^7 to 10^10 CFU/day, though optimal levels depend on specific strains.


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Prebiotics and Synbiotics - Prebiotics: Non-digestible food ingredients (e.g.,

fructooligosaccharides, inulin) that selectively feed beneficial gut bacteria. Synbiotics:

Formulations that combine probiotics and prebiotics, aiming to improve probiotic survival and

colonization [8]. Dietary Fiber: Encouraging whole-grain foods, fruits, and vegetables can

enhance gut flora recovery.
Adjunctive Therapies - Enterosorbents (e.g., activated charcoal, smectite) can help bind bacterial

toxins, though their role in dysbiosis remains under investigation. Zinc Supplementation:

Particularly beneficial in pediatric populations, as zinc supports epithelial integrity and immune

function. Rehydration Therapy: Oral rehydration solutions are key to preventing dehydration in

patients with ongoing diarrheal symptoms.
Prevention and Long-Term Management
Vaccination - Preventing primary infections (e.g., rotavirus vaccine, COVID-19 vaccine,

influenza vaccine) can reduce the need for antibiotics and, hence, lower the risk of dysbiosis.
Infection control - Hand Hygiene and Sanitation: Reduces the transmission of pathogenic

bacteria and viruses. Isolation Precautions: In hospital settings, adherence to infection control

protocols can prevent healthcare-associated infections that complicate the patient’s

gastrointestinal condition.
Ongoing Microbiome Monitoring - In cases of severe or recurrent dysbiosis, periodic stool

analyses or other emerging diagnostics may guide personalized interventions, including targeted

probiotic use or dietary modifications.

DISCUSSION

Antibiotic-induced dysbiosis can prolong or worsen the clinical course of acute intestinal

infections, resulting in additional morbidity and extended convalescence. The gut microbiota

plays a critical protective role, acting as a barrier to pathogen colonization and contributing to

immune function. When antibiotics are necessary for life-threatening infections, the judicious

selection of agents and the concurrent or subsequent use of microbiome-supportive measures can

mitigate the negative impact on the gut ecosystem.
Challenges persist in identifying optimal probiotic strains, dosages, and durations for different

patient populations. Moreover, while current evidence supports the efficacy of many

commercially available products, further large-scale, randomized clinical trials are needed to

standardize best practices, especially in pediatric and immunocompromised individuals.

CONCLUSION

The management of dysbiosis during antibiotic therapy for acute intestinal infections necessitates

a multifaceted approach. Central to this is the rational use of antibiotics guided by precise

microbiological diagnoses, combined with probiotic supplementation, prebiotics, and synbiotics

to help restore intestinal homeostasis. Emphasizing antibiotic stewardship, promoting preventive

measures (e.g., vaccination), and advancing diagnostic techniques will contribute to improved

patient outcomes and a reduced burden of antibiotic-associated dysbiosis.

REFERENCES

1.

World Health Organization. Diarrheal disease.

https://www.who.int

(Accessed Feb 2025).

2.

Francino MP. Antibiotics and the human gut microbiome: Dysbioses and accumulation of

resistances.

Front Microbiol.

2016;7:1437.

3.

Dethlefsen L, Relman DA. Incomplete recovery and individualized responses of the

human distal gut microbiota to repeated antibiotic perturbation.

Proc Natl Acad Sci U S A.

2011;108 Suppl 1:4554–4561.


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4.

Szajewska H, Canani RB, Guarino A, et al. Probiotics for the prevention of antibiotic-

associated diarrhea in children.

J Pediatr Gastroenterol Nutr.

2016;62(3):495–506.

5.

Akhatovna, M. K. (2023). BENEFITS OF COMPLETE TREATMENT OF

INFECTIOUS DIARRHEA IN YOUNG CHILDREN. Ethiopian International Journal of

Multidisciplinary Research, 10(10), 115-117.
6.

Mulladjanova, K. A., & Khodjimatov, G. M. (2021). Algorithm of Diagnosis,

Pathogenetic Treatment and Prevention of Acute Diarrhea in Early Children. Annals of the

Romanian Society for Cell Biology, 25(1), 6355-6363.
7.

Mutalibovich, Q. A. (2024). ENTEROVIRAL INFECTIONS: MODERN FEATURES.

Ethiopian International Journal of Multidisciplinary Research, 11(02), 199-200.
8.

Saidakbarovich, M. A. (2023). INTRAUTERINE INFECTIONS: A MODERN VIEW

AT THE PROBLEM. International Journal of Medical Sciences And Clinical Research, 3(10),

79-82.

References

World Health Organization. Diarrheal disease. https://www.who.int (Accessed Feb 2025).

Francino MP. Antibiotics and the human gut microbiome: Dysbioses and accumulation of resistances. Front Microbiol. 2016;7:1437.

Dethlefsen L, Relman DA. Incomplete recovery and individualized responses of the human distal gut microbiota to repeated antibiotic perturbation. Proc Natl Acad Sci U S A. 2011;108 Suppl 1:4554–4561.

Szajewska H, Canani RB, Guarino A, et al. Probiotics for the prevention of antibiotic-associated diarrhea in children. J Pediatr Gastroenterol Nutr. 2016;62(3):495–506.

Akhatovna, M. K. (2023). BENEFITS OF COMPLETE TREATMENT OF INFECTIOUS DIARRHEA IN YOUNG CHILDREN. Ethiopian International Journal of Multidisciplinary Research, 10(10), 115-117.

Mulladjanova, K. A., & Khodjimatov, G. M. (2021). Algorithm of Diagnosis, Pathogenetic Treatment and Prevention of Acute Diarrhea in Early Children. Annals of the Romanian Society for Cell Biology, 25(1), 6355-6363.

Mutalibovich, Q. A. (2024). ENTEROVIRAL INFECTIONS: MODERN FEATURES. Ethiopian International Journal of Multidisciplinary Research, 11(02), 199-200.

Saidakbarovich, M. A. (2023). INTRAUTERINE INFECTIONS: A MODERN VIEW AT THE PROBLEM. International Journal of Medical Sciences And Clinical Research, 3(10), 79-82.