117
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
IN VITRO STUDY OF THE BIOEFFECTIVENESS OF A TABLET WITH A
SEDATIVE EFFECT
Anvarova M.J.
1
Turdiyeva Z.V.
2
Tashkent Pharmaceutical Institute, Tashkent city, Republic of Uzbekistan
https://doi.org/10.5281/zenodo.17324589
Relevance:
One of the current problems in the pharmaceutical industry is the creation of
innovative, highly bioeffective, affordable, and low-toxic drugs based on local medicinal plant raw
materials, as well as the localization of the production of recommended drugs. Biopharmaceutical
properties of research in the development of new drugs assess their pharmacological effects. The
study of biopharmaceutical properties eliminates doubts about the bioefficacy of the proposed drugs
and the use of finished drugs in terms of their biopharmaceutical properties.
It is known from the
literature that the final stage of research on the composition and technology of a particular drug is
devoted to studying the bioefficacy of the proposed drug. The results of this study, in turn, provide a
guarantee for the quality of the finished product, that is, its bioactivity. Biopharmaceutical research
begins with in vitro experiments and is correlated with in vivo studies. The in vitro method is an
instrumental method and is used in almost 90% of pharmaceutical enterprises in the development of
drugs to conduct biopharmaceutical analyses and study the quality of the finished product.
Purpose of the study:
The biopharmaceutical properties of the recommended sedative tablets
were studied in vitro. These studies were carried out in a "Rotating centrifuge" device. In
experimental studies, factors related to bioefficacy, such as the volume and pH of the solvent medium,
and the speed of the centrifuge rotation, were studied.
Methods and techniques:
In the study of the biopharmaceutical properties of the
recommended tablets in vitro, we studied the factors affecting bioefficacy, such as the volume of the
medium, the pH of the dissolution medium, the effect of the rotation speed of the rotating flask on
the release of bioactive substances. In the next study, the effect of the rotation speed of the flask on
the release of the recommended granules was continued using the "Rotating flask" method. Based on
the sensitivity of the bioactive substances contained in the recommended tablets in the studies, the
solution volume was selected as 1000 ml. The experiments were planned and carried out at speeds of
50, 100, 150 and 200 m/min. The next stage of the research continued with the study of the pH of the
dissolution medium. The dissolution mediums for the experiments were different: purified water -
neutral medium, 0.1 mol/l hydrochloric acid solution - acidic medium and 0.1 mol/l sodium hydroxide
solution - alkaline medium. The results showed that 85.9% of bioactive substances were released in
45 minutes in a neutral medium. It was observed in the studies that this indicator was 66.4% and
58.5% in acidic and alkaline environments, respectively. Based on the research results, it was
determined that a neutral medium for a tablet with a sedative effect is specific for the release of
bioactive substances contained in the tablet into the dissolution medium.
Results:
The obtained indicators show that the release of bioactive substances from the
recommended tablets into a neutral environment reached 54.1% in 15 minutes, 79.4% in 30 minutes,
and 85.9% in 45 minutes.
Conclusions:
Based on biopharmaceutical studies conducted in "in vitro" experiments, the
biopharmaceutical properties of the recommended drugs were determined and the conditions for
conducting the "Solubility" test were proposed.
