ЕВРОСИЁ ПЕДИАТРИЯ АХБОРОТНОМАСИ 1(12) 2022
ISSN 2181-1954. EISSN 2181-1962
UDC 616.36-002
EFFECT OF DARMONAL AND PHYT1N COMPLEX COMPOUNDS ON
CARBOHYDRATES METABOLISM AT EXPERIMENTAL TOXIC HEPATITIS
G.A.Karimova, S.D.Aminov, F.X.Dusmurodova
Tashkent Pediatric Medical Institute
Rezume
The aim of study was to research the effect of darmonal and phytin compounds on the amount of
glycogen and lactic acid in the liver tissues.
Materials and methods. The new compounds of phytin-C were studied: phytin-C, cobalt phytate and
patent substance of darmonal obtained from wheat grain cells grown under special conditions. For
comparison, the bioflavonoid of plant origin Liv-52, being used in treatment of liver diseases, was also
studied. The study was carried out on 60 white rats weighing 120-150 g. The toxic hepatitis was caused
by subcutaneous administration of 50% carbon tetrachlormetan oil solution in dose 0.8 ml/100 g for 4
days.
Results. The results showed that the combined introduction of extract significantly enlarged the
outcome at joint use drug Liv-52 in animals, and, especially such effect was noticeable with addition
phytin-C. The use bioactive substance in combination with complex phytin compound was more effective
for accumulation of glycogen in the liver than using the drugs separately, hepatotoxin - carbon
tetrachlormetan reduced carbohydrate metabolism and led to increase glycogen in liver tissues as well
as the decreased lactic acid. It was determined that the combined treatment was more effective at toxic
hepatitis induced by carbon tetrachlormetan, it had the hepatoprotective effect and improved liver
function.
Key words: substance of darmonal, darmonal A, Liv-52, toxic hepatitis, glycogen, lactic acid.
ВЛИЯНИЕ ДАРМОНАЛА И КОМПЛЕКСНЫХ СОЕДИНЕНИЯ ФИТИНА НА
УГЛЕВОДНЫЙ ОБМЕН ПРИ ЭКСПЕРИМЕНТАЛЬНОМ ТОКСИЧЕСКОМ ГЕПАТИТЕ
1 ’.А. Каримова, С.Д. Аминов, Ф.Х. Дусмуродова
Ташкентский педиатрический медицинский институт
Резюме
Целью исследования является изучение влияния дармонала и соединений фитина на
количество гликогена и молочной кислоты в тканях печени.
Материалы и методы. Изучались новые соединения фитина: фитин-С, фитат кобальта и
патентированная субстанция дармонал, полученное из клеток зерна пшеницы, выращенных в
особых условиях. Для сравнения также был исследован биофлаваноид растительного
происхождения Лив-52, используемый при лечении заболеваний печени. Исследование проведено
на 60 белых крысах массой 120-150 г. Токсический гепатит вызывали подкожным введением
50%>-ного маслянного раствора тетрахлорметана в дозе 0,8мл/100 г в течение 4 дней.
Результаты показывают, что совместное введение экстракта значительно превосходит
таковое при совместном введении препарата Лив-52
у
животных, особенно когда такой эффект
был заметен при совместном введении препарата с фитином-С. При применении биоактивного
вешества в сочетании с комплексным соединением фитина более эффективно приводило к
накоплению гликогена в печени, чем при применении препаратов отдельно, гепатотоксин -
тетрахлорметан снижает углеводный обмен и приводит к увеличению гликогена в тканях печени
и снижению молочной кислоты. Установлено, что комбинированное лечение более эффективно
при токсическом гепатите, индуцированном тетрахлорметаном, оказывали гепатозащитное
действие улучшают функции печени.
Ключевые слова: субстанция дармонал, дармонал А, лив-52, токсический гепатит, гликоген,
молочная кислота.
82
ЕВРАЗИЙСКИЙ ВЕСТНИК ПЕДИАТРИИ 1(12) 2022
ISSN 2181-1954. EISSN 2181-1962
BIRIKMALARINING UGLEDOD ALMASHISHIGA TA’SIRI.
G.A. Karimova, S.D. Aminov, F.X. Dusmurodova
Toshkent pediatriya tibbiyot institute
Rezyume
Tadqiqotning maqsadi darmonal va jltin birikmalarining jigar to'qimalarida glikogen va sut kislotasi
miqdoriga ta 'sirini о 'rganishdir.
Materiallar va usullar. Yangi fltin birikmalari o'rganildi: fitin-C, kobalt fitat va maxsus sharoitlarda
yetishtirilgan bug'doy don hujayralaridan olingan patentiangan darmonal moddasi. Taqqoslash uchun,
jigar kasalliklarini davolashda qo'llaniladigan o'simlik kelib chiqishi Liv-52 bioflavonoidi ham
o'rganildi. Tadqiqot 120-150 g og'irlikdagi 60 ta oq kalamushlarda o'tkazildLToksik gepatit 4 kun
davomida 0,8 ml/100 g dozada uglerod tetrakloridning 50% yog'li eritmasini teri ostiga yuborish orqali
qo'zg'atildi. Natijalar shuni ko'rsatadiki, ekstraktni birgalikda qo'llash Liv-52 ni hayvonlarga bir vaqtda
qo'llashdan sezilarli darajada ustundir, ayniqsapreparat fitin-C bilan birgalikda qo'llanganda bunday
ta'sir ko'rsatilganda. Biofaol moddani fitinning murakkab birikmasi bilan birgalikda qo'llashda и dori-
darmonlarni alohida qo'llashdan ko'ra jigarda glikogenning to'planishiga olib keldi, gepatotoksin -
uglerod tetraklorid uglevod almashinuvini pasaytiradi va jigar to'qimalarida glikogenning ko'payishiga
olib keladi. va sut kislotasining kamayishi.
Uglerod tetraxlorid qo'zg'atadigan toksik gepatitda kombinatsiyalangan davolash samaraliroq
ekanligi, gepatoprotektiv ta'sir ko'rsatishi va jigar faoliyatiniyaxshilashi aniqlandi.
Kalit so 'zlar: darmonal, darmonal A, liv-52, toksik gepatit, glikogen, sut kislotasi.
Relevance
The study of biologically active substance, their
introduction in medical practice have great
significance for increase population’s work ability
and prophylaxis of various pathological states. In
this relation the great interest have biologically
active substances taken from wheat. For example,
sumalyak which was used by Uzbek people from
ancient time in spring, but it has some lack, it is not
possible to use for long time. The new biologically
active substance on the base of wheat was taken as
small powder - darmonal, and, on the base of oats
it
was
darmonal-A
(Mahmudjonova
K.S.,
Karimova
S.A.,2002)
in
Pharmaceutic
institute.
The
pharmacological properties of darmonal were
caused with content of wide spectrum various
bioactive compounds: enzymes, complex В
vitamins, proteins, fats, carbohydrates. Patent №
(GOST 7169-66).
’
The
liver
participation
in
carbohydrate
metabolism has particular significance. One of its
activity is keeping glucose in hepatocytes by its
transformation in glycogen and discharge in blood,
when the reserve is exhausted. It is known that
enzymes
activity
depends
on
microsomal
oxydation
of
glucose-6-phosphate
on
pentozophosphate way and amount of glycogen.
The glycogen is mainly kept in the liver, muscles,
and, it is glucose reserve. But, when glycogen
supplys myocytes with glucose-6-phasphate, in
liver the glycogen gives glucose itself and other
peripheric tissues.
Therefore, the glycogen amount decreasing in
liver reduce coming NADF.N in chain of
microsomal oxydation. It leads to retardation of dis-
intoxication processes. The decrease activity of
fermentative system at liver disease, in its turn,
leads to reduce glycogen production and increase
lactic acid, disorder of carbohydrate metabolism
[3]. At toxic hepatitis the tissue hypoxia develops
on the account of microcirculatory changes. It takes
to decrease aerobic glycolis and predomination of
anaerobic glycolis [2,4]. The high assimilate of
glucose and accelerated glycogenolis are typical for
it. Moreover the amount of lactic acid increases in
cells and milk- sour hyperacidity is developed.
Therefore, one of the most actual problem is the
search of drug and bioactive substances with
hepatoprotective properties made from natural
substances, study effects of their combined
application and use in practice.
Aim of our study
is research influence of
darmonal substance on phytin compounds on the
amount of glycogen and lactic acid in liver tissues.
Materials and methods
There were researched the new phytin
compounds: phitin-C, cobalt phytate and darmonal,
Patent № (GOST 7169-66) which was taken from
wheat grains being grown in special conditions. For
comparison the bioflavonide of
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ЕВРОСИЁ ПЕДИАТРИЯ АХБОРОТНОМАСИ 1(12) 2022
ISSN 2181-1954. EISSN 2181-1962
vegetable origin Liv-52, applicating at hepatic
diseases, were used [1].
He study was carried out on 60 white rats with
weight from 120 to 150gr. The toxic hepatitis was
caused by subcutaneous introduction of 50% oiled
tetrachlormetan solution in dose 0,8 ml/100 g for 4
days [5]. All experimental animals with toxic
hepatitis were divided into 10 groups: 1 was intact;
11 was control (group with toxic hepatitis caused
by tetrachlormetan); 111 group had darmonal
extract in dose 10 mg/kg; IV group of animals got
darmonal extract in dose 100 mg/kg; V group had
100 mlg/kg of darmonal-A; VI group got phytin-C
in dose 100 mg/kg; Vll had darmonal extrat and
cobalt phytate in dose 200 mg/kg; VIII group of
animals got darmonal extract and phytin-C
in dose
100 mg/kg;
IX group had darmonal extract 100
mg/kg and phytin 100 mg/kg; X group got Liv-52
in dose 100 mg/kg.
These drugs were introduced experimental
animals per orally every day. As intact group were
taken 6 healthy rats. On the 10
th
day of study
laboratory animals were decapitated under
anesthesia then the glycogen was determined in
liver tissue by(Zeifter S. et al., 1950), and lactic
acid by Byuhner G.Dj. (1965). The taken results
were undergone to statistic processing [6].
Results and discussion
At animals of 1 group the amount of glycogen
in liver tissues was decreased to 43,7%, but lactic
acid increased in in 2,3 times (in comparison with
the same intact animals). The taken results showed
that acute poisoning with tetrachlormetan led to
accumulation of lactic acid in the liver. At
simultaneously
introduction
of
researching
substances
for
prophylaxis
of
hepatitis
development and tetrachlormetan the prevention of
sharp decrease glycogen amount and increase lactic
acid in liver tissues were observed. There was
particular increase of glycogen and reduce lactic
acid in tissues of animals from III and IV groups.
Moreover, the darmonal action in dose 10
mg/kg was weak and the results of experiments
were statistically incorrect, later, and, further the
substance was introduced in the dose 100 mg/kg
and the drug increased the level of liver glycogen
on 29,6%, lactic acid on 41,4%. In V and VI groups
the glycogen content in liver tissue increased on
44,7 and 45,5%, lactic acid was decreased to 60%.
At animals of IX group the hepato-protector Liv-52
made the same activity increasing glycogen content
on 46,6% and lactic acid on 56%.
Introduction
of
darmonal
with
phytin
compounds enhanced their general hepatotropic
action. Simultaneously intake phytin, cobalt
phytate and phytin-C with preventive purpose led
to increase the amount of glycogen in liver tissue
and decrease the level of lactic acid. Particularly the
joint intake of cobalt phytate and phytin-C led to
increase glycogen led on 56,6 and 57%, that
witnessed on improvement of carbohydrate
metabolism and reduce lactic metabolism on 65,4
and 64,4% accordingly.
Influence of drug and phytin compounds on level of glycogen and lactic acid in liver
tissues at experimental toxic hepatitis, M±m
Group
Glycogen, gr/1
Lactic acid, mmol/1
Intact
49,2±0,92
l,5±0,2
Control group CCl
4
0,8ml/100 g
27,7±1,1
5,0±0,76
Darmonal 10 mg/kg
31,26±0,83*
3,85±0,43
Darmonal 100 mg/kg
35,9 ±1,2
2,93 ±0,47
Darmonal-A 100 mg/kg
40,l±l,15
2,0±0,14
Phytin-C 100 mg/kg
40,36±0,56
2,01±0,13
Darmonal 100 mg/kg+Cobalt
phytate 200 мг/кг
37,6±1,2
2,34±0,15
Darmonal 100 mg/kg+Фитин-С
100 mg/kg
43,4±l,0
l,73±0,17
Darmonal 100 mg/kg+Phytin 100
mg/kg
43,5±0,92
l,78±0,09
Liv.52 100 mg/kg
40,65±0,77
2,2±0,09
Indices of control group R
=
Q
Joint introduction darmonal with phytin
compounds led to synergistic effect.
The results show that joint introduction extract
considerably surpass the outcomes at adding the
medicine Liv-52 in animals, especially such effect
was noticed at joint introduction with phytin-C.
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ЕВРАЗИЙСКИЙ ВЕСТНИК ПЕДИАТРИИ 1(12) 2022
ISSN 2181-1954. EISSN 2181-1962
In our opinion the efficacy of general
hepatotropical action may be depended on plenty of
organic and inorganic biologically active sustances
in content of drug substances, antihypoxant,
antioxidant и hepatoprotector activity of phytin
compounds. At toxic hepatitis the tissue hypoxia
was developed on the account of microciculatory
changes. It led to decrease of aerobic glycolysis and
predominance of anaerobic glycolysis. For this it is
typical the high mastering of glucose accelerated
glycogenolysis. Besides, the amount of lactic acid
increases in cells and the milk sour acidosis occur.
On this base the amount of lactic acid was
determined in liver tissue (pic.2)
At use drug darmonal in combination with
complex phytin compound led more effectively to
glycogen accumulation than at applicationn them
separately, hepatotoxin - tetrachlormetan reduced
carbohydrates metabolism and led to glycogen
increase in liver tissue and diminish lactic acid.
It means that use darmonal extract at animals
with hepatitis leads to restore the function of
glycogen accumulation in the liver. By this feature
doesn't yield Liv-52 and phytin-C.
Picture 2. Influence of new drugs on amount of lactic acid in liver at experimental hepatitis
Picture 1. Influence of new drugs on the glycogen amount in liver at experimental hepatitis
ЕВРОСИЁ ПЕДИАТРИЯ АХБОРОТНОМАСИ 1(12) 2022
ISSN 2181-1954. EISSN 2181-1962
Therefore, it is purposefully to take darmonal in
combination with phytin-C and cobalt phytate,
because such complex leads to restore the function
glycogen accumulation in animals liver.
Conclusions
1.
At toxic hepatitis, being caused with
tetrachlormetan, the darmonal substance and
complex phytin compound protect liver from
damage and disorder glycogen biosynthesis in
liver tissue and increase lactic acid.
2.
The combined application of darmonal and
complex phytin combound leads to increase the
hepatoprotective action.
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Entered 09.02.2022
