European Journal of Medical Genetics and Clinical Biology
Volume 1, Issue 5 | 2024
https://journal.silkroad-science.com/index.php/JMGCB -
85
https://doi.org/10.61796/jmgcb.v1i5.428
BIOLOGICAL PRODUCT - BIOINSECTICIDE
Pulotov F.S, Sayfiddinov K.F, Abdukhalimova Sh.Sh.
Doctor of Philosophy of Veterinary Sciences, (PhD), senior
researcher
student at Samarkand University of Veterinary Medicine, Animal
Husbandry and Biotechnology.
student of Samarkand University of Veterinary Medicine, Animal
Husbandry and Biotechnology.
nivi@vetgov.uz
Received: March 10, 2024; Accepted: Apr 10, 2024; Published: Mei 10, 2024;
Abstract
:
In this article Bacillus thuringiensis var.thuringiensis on the bacterial strain
thuringiensis Uzviti М №1, data are presented on the research work on the study of the
insecticidal properties being carried out at the same time on the experimental series of the
biological drug bioinsecticid, which was first prepared in a laboratory setting.
Keywords
:
Bioinsecticide, biological method, insecticide, insect, mite, bacterium, spore,
strain, bacillus thuringiensis, entomoside.
This is an open-acces article under the
CC-BY 4.
0 license
Introduction
Scientific institutions and scientists are informed about parasites and parasites common in the
republic, as well as dangerous vector-borne diseases of humans and animals. The task is to study ways
and means of combating arthropods (blood-sucking insects and ticks) that are safe for the
environment, human and animal health, beneficial fauna and flora, which are carriers of pathogens -
plague, tick-borne encephalitis, tularemia, hemorrhagic fever, Termiz and Tomdi fever, ephemeral
fever, tick fever, cholera, relapsing and typhus, malaria, trypanosomiasis, leishmaniasis, anthrax and
many other diseases. In the fight against them, the use of biological methods instead of chemicals is
becoming increasingly important. Our study of a bioinsecticidal drug is one of the efforts in this
direction.
Therefore, the research and implementation of modern and highly effective methods and means
of combating harmful insects and mites is an urgent task. Their solution makes it possible to create a
healthy epizootological and epidemiological situation in populated areas and livestock farms, a clean
ecological environment and a balance between people and animals.
Purpose of the study. An experimental series of the drug “Bioinsecticide” in laboratory
conditions to determine the insecticidal effectiveness against lice beetles of farm animals.
Research methods. In veterinary medicine, microbiological, parasitological, faunistic,
ecological, phenological and other studies are accepted (V.A. Dogel, V.N. Beklemishev, V.V.
Yakhonto, S.D. Pavlov, A.R. Ruzimurodov and other researchers) with using methods, aids and
detectors.
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Experiment results. Bioinsecticide is a biological preparation based on the thuringiensis variant
of Bacillus thuringiensis with crystal-forming spores. Bacillus thuringiensis var.Thuringiensis M No.
1 Bacterial strain of the Veterinary Scientific Research Institute of Uzbekistan, Musca Domestica
vicina Mcq. (house fly) was isolated from the larvae of the local population and included in the
collection of microorganisms of the Veterinary Scientific Research Institute under registration
number No. 1 (2000). It has been established that this strain forms good and specific colonies on
nutrient media with the addition of bacteria MPA, MPB, “Blood” and “Blood Serum”. In these test
tubes, flasks and mats, purely isolated strains of Bacillus thuringiensis var.thuringiensis were
propagated on nutrient media, the initial experimental series of the Bioinsecticide preparation was
prepared, and its insecticidal properties were studied in the laboratory. At the same time, various
concentrations of the drug “Bioinsecticide” were prepared, namely 0.225, 0.45, 0.9, 1.35, 1.8, 2.25,
3.6, 4.05, 4.5, 9.0 , 13.5, 18.0, 22.5 billion/ml of bacterial spores using special titer detectors, and in
laboratory conditions they were sprayed from an experimental preparation onto filter paper placed in
a Petri dish using a dispenser sprayer, and on the surface 30 specimens of larvae and adults of newly
caught lice beetles were placed on this medicinal filter paper, and as a result of test experiments in
order to determine the minimum effective (100 percent) concentration of these drugs, the following
tests were carried out:
experiment 1: The filter paper was placed in 3 Petri dishes, and the surface of each filter paper
was treated with 3.8 ml of “Bioinsecticide” with 0.225 billion/ml bacterial spores. 30 freshly collected
larvae were released onto the surface of this medicinal filter paper, and after 10 minutes the insects
were packaged in Petri dishes with clean filter paper and kept in optimal conditions, that is, in a
thermostat at a temperature of +28+35 0C, every 1, 3, 6, 24 observation work was carried out for an
hour and 5 days;
experiment 2: The experiments were carried out as indicated above, a total of 0.45 billion/ml of
bacterial spores of the "Bioinsecticide" preparation were tested;
experiment 3: The experiments were carried out as indicated above, a total of 0.9 billion/ml of
bacterial spores of the "Bioinsecticide" preparation were tested;
experiment 4: The experiments were carried out as indicated above, a total of 1.35 billion/ml of
bacterial spores of the "Bioinsecticide" preparation were tested;
experiment 5: The experiments were carried out as indicated above, a total of 1.8 billion/ml of
bacterial spores of the "Bioinsecticide" preparation were tested;
experiment 6: The experiments were carried out as indicated above, a total of 2.25 billion/ml of
bacterial spores of the "Bioinsecticide" preparation were tested;
experiment 7: The experiments were carried out as indicated above, a total of 3.6 billion/ml of
bacterial spores of the "Bioinsecticide" preparation were tested;
experiment 8: The experiments were carried out as indicated above, a total of 4.05 billion/ml of
bacterial spores of the "Bioinsecticide" preparation were tested;
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experiment 9: The experiments were carried out as indicated above, a total of 4.5 billion/ml of
bacterial spores of the "Bioinsecticide" preparation were tested;
experiment 10: The experiments were carried out in the same way as above, only using pure
water.
The results of the experiment were determined after 2-5 days and the number of dead and
surviving lice eaters was calculated. Depending on the number of spores contained in the preparation,
the minimum or maximum concentrations NLK0 (non-lethal concentration), LK50 (50% lethal
concentration) and LK100 (100% lethal concentration) were determined.
Each concentration was repeated 3 times. The effectiveness of the drug was also determined
depending on the rate and amount of death of lice beetles. Here,
0 percent of insects in experimental group 1;
20 percent of insects in experimental group 2;
30 percent of insects in experimental group 3;
50 percent of insects in experimental group 4;
70 percent of insects in experimental group 5;
80 percent of insects in experimental group 6;
90 percent of insects in experimental group 7;
100 percent of insects in experimental group 8;
100 percent of insects in experimental group 9 died;
100 percent of the insects in control group 10 were alive (Table 1).
Experience in studying the insecticidal effect of various concentrations of the
drug “Bioinsecticide” in laboratory conditions
S.n.
Drug
concentration
(billion/ml
bacterial spores)
Number of lice
eaters who took
medications
(copy)
Number of lice
eaters that died
after 2-5 days
(copy)
Efficiency
(percentage)
1
0,225
30
0
0
2
0,45
30
6
20
3
0,9
30
9
30
4
1,35
30
15
50
5
1,8
30
21
70
6
2,25
30
24
80
7
3,6
30
27
90
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8
4,05
30
30
100
9
4,5
30
30
100
10
Control (treated
with clean water)
30
0
0
As a result, the minimum and 100% effective concentration of the drug “Bioinsecticide” with 4.05
bacterial spores on the 2nd day against lice beetle larvae and the concentration of bacterial spores at
a concentration of 4.5 billion/ml turned out to be 100% insecticidal in laboratory conditions by the
3rd days compared to imago forms
Conclusion
For the first time, an experimental series of the drug “Bioinsecticide” was created in laboratory
conditions and it was found that a concentration of 4.05 billion/ml of bacterial spores is 100%
effective against the larvae of lice beetles of farm animals, and a concentration of bacterial spores in
a concentration of 4.5 billion/ml is effective against imagoes. forms 100%.
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