I Конгресс детских врачей Республики Узбекистан
с международным участием
«Актуальные вопросы практической педиатрии»
305
COMPOSITION OF THE INTESTINE MICROFLORA IN YOUNG
CHILDREN UNDER CONDITIONS OF DIARRHEA AND RECOVERY
Turdieva Sh.T., Abdurashidova Kh.B.
Tashkent Pediatric Medical Institute, Tashkent, Uzbekistan
INTRODUCTION
The problem of diarrhea in children of different ages remains one of the
topical areas of modern medicine. The study of the characteristics of the
microflora in diarrhea is associated with the development of therapeutic and
preventive measures for this condition. The intestinal microflora performs
important functions in the child's div, including the exchange of energy
substrates and the formation of intestinal immunity. At the same time, the
intestinal microflora changes during the first years of life. In addition to age-
related changes, gut flora changes with nutritional status, birth path, antibiotic
exposure, genetic factors, and episodes of diarrhea.
PURPOSE OF THE RESEARCH
Comparison of the characteristics of the intestinal microflora during and
after treatment of diarrhea in young children.
MATERIALS AND METHODS
The intestinal flora in the feces was studied in 42 children aged 12 months
to 3 years. Diarrhea was defined as an increase in the frequency of stools to at
least three loose stools in 24 hours or as changes (bloody, very loose, or watery).
Feces were collected during the episode of diarrhea and 2 months after the last
episode of diarrhea. Fecal samples were taken in a sterile plastic container from
a soiled baby diaper. Rotaviruses and adenoviruses were determined using
enzyme immunoassay, noroviruses, and saroviruses
–
by polymerase chain
reaction, bacterial enteropathogens
–
by traditional cultivation method.
RESULTS
When studying the features of episodes of diarrhea, it was noted that the
maximum number of bowel movements in 24 hours was from 4 to 6 times
(4.2±1.22).
At the same time, 28.6% (12/42) had sub-febrile div temperature
against the background of diarrhea, 26.2% (11/42) vomited, and 9.5% (4/42)
received antibiotic therapy during the period of diarrhea. In the study of
enteroflora during an episode of diarrhea, an enteropathogen was detected in
61.9% (26/42) of children. At the same time, in 14.3% (6/42) infection of a shifted
category was detected, that is, both bacterial and viral enterobiota were detected
at the same time. Bacterial enteropathogens were detected in 16.7% (7/42) of
children, viral
–
in 28.6% (12/42), and parasitic
–
in 2.4% (1/42) of children. Viral
enteropathogens identified in diarrhea stool samples included norovirus (12/42),
rotavirus (4/42), and sapovirus (2/42).
I Конгресс детских врачей Республики Узбекистан
с международным участием
«Актуальные вопросы практической педиатрии»
306
Bacterial enteropathogens identified in diarrhea stool samples included
E.coli (9/42), Shigella flexneri (2/42), and Campylobacter spp. (2/42). Feces were
re-examined 2 months after the last episode of diarrhea. At the same time, in the
recovery period, enteropathogens were partially preserved. In particular,
enteropathogens of viral etiology were found in 7.1% (3/42), bacterial
–
14.3%
(6/42), and mixed category
–
11.9% (5/42) of children. The microbial
composition both during diarrhea and after recovery was dominated by
proteobacteria, cyanobacteria, actinobacteria, bacteroids, and verrucomicrobes.
In addition, an increase in the number of lactobacilli was found at the end of
episodes of diarrhea, and then there was a decrease in their number in the second
month after the last episode of diarrhea.
CONCLUSIONS
In the etiopathogenesis of diarrhea in young children, along with bacterial
microflora, enteropathogens of viral etiology are of great importance, they
account for up to 28.6% of cases of diarrhea in these children.
