Annals of R.S.C.B., ISSN: 1583-6258, Vol. 25, Issue 1, 2021, Pages. 6440 – 6445
Received 15 December 2020; Accepted 05 January 2021.
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Assessment of the Status of Local Immunity in Patients with Acute
Purulent Odontogenic by Osteitis of the Jaw
N.N.Kayumova
1
, A.A.Khadzhimetov
2
, Sh.M.Khasanov
3
, B.A.Khalmanov
4
Tashkent State Dental Institute
ABSTRACT:
The aim of this study was to study the characteristic features of local and systemic immunity
in patients with acute purulent odontogenicostitis of the jaw. We examined 38 patients with
acute purulent odontogenicperiostitis, who applied to the TSSI clinic. Clinical hematological
immunological research methods were used. It was revealed that the most frequent
associations of microorganisms in acute purulent odontogenicostitis of the jaws are
Staphylococcus aureus, Staphylococcus epidermicus, registered in more than half of the
cases. There was an increase in immunoglobulin G in the blood in acute purulent
odontogenicostitis.
Key words:
acute purulent odontogenicosteitis, microflora of the oral cavity, blood counts,
immunogram.
INTRODUCTION.
Recently, there has been a tendency to increase the number of periostitis in young patients. At
the same time, periostitis accounts for about 80% of all cases of temporary disability in dental
diseases. Analyzing the literature sources of recent years, we can note significant changes in
the local microbial landscape in the oral cavity, which is caused by the impact of a number of
reasons: unfavorable environmental conditions, uncontrolled intake of pharmaceutical drugs,
an unbalanced diet, and an increase in the number of patients with somatic diseases. The
intensity of the course of the pathogenesis of odontogenic inflammation depends on the well-
coordinated interaction of all immune factors. Violation of immunity, among other things, in
the presence of concomitant diseases, increases the possibility of developing multiple
periapical chronic foci. Competitive relations between representatives of the microbiocenosis,
as well as the products of their metabolism, can influence the interaction of microorganisms
with epithelial cells (4,13,19,23). Along with epithelial cells, neutrophils and macrophages,
lymphoid follicles lying within the epithelial layer are of great importance in the local
nonspecific protection of the mucous membranes. At the same time, the intensity of the
pathogenesis of acute odontogenic inflammation depends on the well-coordinated interaction
of two protective mechanisms: local and systemic immunity.
RESEARCH MATERIALS AND METHODS.
The clinical material was based on the data obtained during the examination and treatment of
38 patients with acute purulent odontogenicostitis who applied to the TSDI clinic. Our choice
fell on this form of the disease due to the fact that according to the literature there is a
significant increase in the number of patients with this pathology (by 40%) from the total
number of patients with odontogenic inflammatory diseases. The age of the selected patients
ranged from 4.5 to 6 years. This choice here was due to the fact that this age period is
characterized by a normergic variant of the immune response. The observation groups
included patients who did not have a developed general somatic pathology that could
influence the course of the studied pathology. The disease developed more often in the lower
jaw (74.3%) than in the upper jaw (25.7%), which corresponds to the information presented
Annals of R.S.C.B., ISSN: 1583-6258, Vol. 25, Issue 1, 2021, Pages. 6440 – 6445
Received 15 December 2020; Accepted 05 January 2021.
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earlier in the literature. To evaluate and compare the results obtained during the treatment of
patients, an obsheklinical examination was performed, as well as laboratory
(microbiological), cytological (smear - scraping), bacteriological (inoculation from a purulent
wound) and enzyme immunoassay were used. General clinical observations included:
clarification of the patient's complaints, anamnesis of the disease and life, local examination,
palpation of tissues, percussion of causal teeth.
The material for microbiological studies of the microflora of a purulent wound was the
contents of the wound taken with two sterile cotton swabs. The concentration of
immunoglobulin G, M, and A in the blood serum was studied by the enzyme immunoassay
on an analyzer. The level of circulating immune complexes was studied in the blood using
7% polyethylene glycol and expressed in conventional units.
RESEARCH RESULTS AND DISCUSSION .
One of the most important factors of virulence and pathogenicity of S. Epidermidis and S.
Aureus is the ability to form biofilms. When a certain density of cells attached to the surface
is reached, the biofilm maturation stage occurs, during which the actual structure of the
biofilm is formed due to the synthesis of polysaccharides by the cell. It should be noted that
staphylococci produce a wide range of toxins: various enterotoxins, hemolysins, toxins,
enzymes (lipase, phospholipase, metalloprotease, cytolytichemolysins), the C5 complement
protein, as well as some other proteins, which indicates its possible participation in the
processes of protecting microbial cells from the human immune system. In this situation,
odontoblasts are the first line of defense cells that control all the metabolic processes of the
tooth pulp in normal and inflammatory conditions (24,25,26).
In pediatric practice, it is believed that in children, the chronic stage of pulp inflammation
often develops not as a result of the transition from the acute stage, but as a primary-chronic
process (20,21). Our studies have shown that the inflammatory process in the pulp of
temporary teeth proceeds according to the type of acute reaction. Therefore, the symptoms of
inflammation of the tooth pulp in children are pronounced for several hours, and then an
abcess develops in the periapical tissues. Acute inflammation begins with a spasm of the
arterioles under the action of catecholamines and vasoactive proteins-endothelins. Venous
stasis leads to a change in the redox processes in the pulp and to the development of oxygen
deficiency. Hypoxia is accompanied by the accumulation of organic acids in the cytoplasm of
pulp cells. Due to the increasing osmotic pressure, there is pain in the tooth. An important
role in the development of pain response in inflammation of the tooth pulp is also played by
the irritation of the receptor endings by products of anaerobic glycolysis and the increase in
their pain sensitivity by inflammatory mediators. This applies primarily to the presence of
soreness of the affected jaw during palpation and movement, hyperemia of the oral mucosa
on the affected side, infiltration and swelling of soft tissues on the affected side. The
frequency of these objective local symptoms is representatively higher than the others
detected in patients during admission to the inpatient department.
At the 2nd stage of the research, we conducted a microbiological study. The main causative
agents of acute purulent odontogenicostitis of the jaws isolated in patients are presented in the
table. As can be seen from the presented research results, the most frequent associations of
microorganisms in acute purulent odontogenicostitis of the jaws are Staphylococcus aureus,
Staphylococcus epidermicus, registered in more than half of the cases. Among the studied
associations, Streptococcus oralis is less often distinguished.
Annals of R.S.C.B., ISSN: 1583-6258, Vol. 25, Issue 1, 2021, Pages. 6440 – 6445
Received 15 December 2020; Accepted 05 January 2021.
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Таble -1
The main causative agents of acute purulent odontogenicostitis of the jaws isolated from
patients (Р±тр, %)
Name of the microorganism
Detection frequency
Staphylococcus aureus
72,4±4,2
Staphylococcus epidermicus
48,2±4,0
Staphylococcus haemoliticus
24,9±2,5
Streptococcus pyogenes
37,5±4,7
Streptococcus oralis
21,2±4,0
Pneumococcus niger
4,6±2,0
Pneumococcus aeruginosa
2,8±1,6
Bacteroidus spp.
5,1±2,1
Enterobacteriaceae
3,2±1,7
Escherichia coli
1,4±0,6
Thus, a bacteriological study of the wound discharge in patients with acute purulent
odontogenicostitis showed that the basis of the microflora in the species ratio is
staphylococci. These microorganisms accounted for 82 % of the total number of isolated
strains.
In the studies of I. G. Ostrovskaya (2017), it was shown that IGG gives the greatest
specificity to immune responses, highly effectively enhances phagocytosis and complement-
dependent lysis, and also participates in the neutralization of adhesive pathogen molecules in
the site of inflammation. In addition, the staphylococcus cell wall contains protein A, which
can strongly bind to the Fc fragment of the immunoglobulin molecule, while the Fab
fragment remains free and can bind to a specific antigen.
Table - 2
Indicators of immunoglobulins and circulating immune complexes in the blood serum of
patients with acute purulent odontogenicostitis of the jaw
The parameter under study
Patients
with
acute
odontogenicostitis of the
jaw
n=12
Healthy faces
n=12
Иммуноглобулин А IgА, г/л
0,89±0,09*
1,54+0,14
Иммуноглобулин GIgG, г/л
16,04+0,67*
11,34+0,98
Annals of R.S.C.B., ISSN: 1583-6258, Vol. 25, Issue 1, 2021, Pages. 6440 – 6445
Received 15 December 2020; Accepted 05 January 2021.
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Иммуноглобулин Е IgЕ, г/л
61,63+5,14
65,38+4,79
Иммуноглобулин М IgМ, г/л
2,54+0,23*
1,67+0,16
Циркулирующие иммунные
комплексы у.е.
77,83+6,38*
45,29+3,58
Note: * - significance of differencesР<0,05
More significant was the decrease in the level of IdA due to the development of acute
purulent odontogenicostitis of the jaws compared to the control group, which is probably due
to the increased synthesis of secretory immunoglobulin A. However, the concentration of
IdM at the systemic level in patients is slightly increased. The level of circulating immune
complexes in the blood of the representatives of the main group increased representatively.
Thus, an imbalance in the "foreign agent - immune defense" system in the oral cavity can
cause inflammation in the oral mucosa. As indicated by the obtained research results, in acute
purulent odontogenicostitis, the ability of periodontal cells to recover is not observed, but
rather indicates the development of irreversible processes, which requires surgical
interventions and appropriate pharmacological correction.
CONCLUSIONS
1.
In the clinical examination of patients with acute purulent odontogenicostitis of the jaws,
a high frequency of individual local symptoms was established. The most frequent
associations of microorganisms in acute purulent odontogenicostitis of the jaws are
Staphylococcus aureus, Staphylococcus epidermicus, registered in more than half of the
cases.
2.
This pathology is accompanied by a shift in the parameters of white blood and marked
hemic hypoxia is accompanied by increased levels of lactic acid in the cells, which may
be one reason for the violation of local microcirculation and the accumulation of
endogenous toxins.
3.
An increase in immunoglobulin G in the blood in acute purulent odontogenicostitis is
apparently associated with a violation of microcirculation and in this situation, it may not
come from the blood plasma, or the formation of stably bound complexes of
immunoglobulins with antigens occurs.
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