This article analyzes the synthesis of the Tenebrio molitor protein (Coleoptera: Tenebrionidae) belonging to a food insect, depending on its nutrient content. Differences in protein synthesis by Tenebrio molitor larvae were shown when wheat bran and flour from the macrophytesLemna minor and Azollacarolina were added. In particular, it was noted that TMO-2 and TMO-6 T.molitor F6 larvae are synthesized on average 31.02% protein in wheat bran, 38.13% in duckweed and 30.87% in azole. It was found that the larvae of variant F6 grown on wheat bran synthesized protein on average 4.79% less than the variant F1 of wheat. F6 larvae grown in duckweed synthesized 7.11% more protein than larvae grown in wheat bran and 0.15% less protein synthesis than larvae grown in azole. It was found that F6 larvae grown in duckweed produce 7.26% more protein than larvae grown in azole. Summarizing these indicators, it was noted that in the body of larvae eating macrophytes insufficient absorption of nutrients, low moisture content in the feed led to their death, egg laying and larvae of variant F6 produced significantly less protein than variant F1. When feeding macrophytes, it is advisable to takeintoaccountits moisture content or add flour based on them to other food sources. It was studied that egg-laying larvae grown on the basis of azole accounted for 38.14%, whereas in duckweed this indicator was 58.38%. It was found that in larvae grown on wheat bran, the percentage of egg-laying is 68.85%. The mortality rate of larvae feeding on wheat bran was 18.22%, in duckweed - 44.63%, in azole - 62.27%, this is due to insufficient moisture in the composition of duckweed and azole (average humidity 3.4-4.2%), relatively high humidity (average humidity 9.58-10.12%) in wheat bran. Survival coefficients of Tenebrio molitor F6 larvae were determined in three different compositions and sources of nutrients with different humidity (wheat bran, Lemna minor and flour Azollacarolina). Tenebrio molitor F6 larvae of the generation of variants TMO-2 and TMO-6 were grown on wheat bran with an average survival rate of 0.79, on duckweed flour - 0.50, on azolla flour - 0.64. It was noted that the survival rate of larvae grown on wheat bran was 15.5% higher than that of larvae grown on azolla, and it is advisable to explain the source not by the protein reserve, but by the amount of moisture in it. It was noted that the moisture content (9.58-10.12%) (protein 15%) in wheat bran was a factor in the high viability of the larvae compared to azolla (protein-27%, humidity 8.2%). Despite the easy protein synthesis in the body of larvae in duckweed flour (protein - 16.1%, humidity - 4.4%) compared to azolla flour, its content is 3.8% lower than that of azolla, due to the fact that survival the larvae in them were lower than in wheat and azolla. Therefore, along with the protein content, moisture is an important factor when choosing a food source. When summing the survival rates of larvae of the Tenebrio molitor F6 generation in the studied nutrient media, the average survival was calculated as 0.96. This means that based on the established ratios of wheat bran, duckweed and azolla flour, it will be possible to create a nutrient medium with a new content, high nutritional value and low cost
Poultry farming is one of the most developed industries, and it is very important to operate the industry completely without waste. More than 20,000 chickens are slaughtered in poultry factories per month. Taking into account that on average 100 grams of feathers are extracted from one chicken, the main goal is to extract keratin protein by processing more than 2 tons of chicken feathers per month. During the research, my method of extracting protein from feathers will be discussed. In addition, determination of the amount of nitrogen in the protein isolated by a special method, determination of the total protein and amino acids are discussed. In the results section, it was found that 25% dry mass was extracted, it contained 17 different amino acids. It was concluded that chicken feather protein can be used for cosmetics based on theoretical comparisons with keratin protein. Hair is composed mainly of keratin protein and a small amount of lipid.
Glioblastoma is a highly aggressive and difficult-to-treat brain cancer with limited therapeutic options. This study aims to design and optimize protein nanoparticles loaded with Everolimus, a potent anticancer drug, for targeted therapy of glioblastomas. The protein nanoparticles were formulated using a biodegradable protein matrix and characterized for physicochemical properties, drug encapsulation efficiency, and drug release kinetics. Cellular uptake studies were conducted to assess the internalization of nanoparticles by glioblastoma cells. The results demonstrate uniform size distribution, stable surface charge, high drug loading efficiency, sustained drug release, and efficient cellular uptake of Everolimus-loaded protein nanoparticles. This research highlights the potential of protein nanoparticles as a promising strategy for improving the treatment outcomes of glioblastomas.
Strategic planning is an integral part of the organization’s functioning system. Successful development of the enterprise is impossible, and effective strategic planning is also impossible without matrix tools. The matrix method used in strategic planning allows you to visually present the information being studied, as well as determine the correct line of behavior in a particular situation. Matrix tools, for developing an enterprise development strategy is a set of
economic forecasting tools that allow to fully implementing the enterprise strategy in a market economy.
Reactive arthritis is an inflammatory lesion of the joints that occurs as a reaction to the invasion of the body by any infectious agents. The high social significance of nosologies dictates the need to search for reliable cartilage biomarkers that have diagnostic value not only in recognizing degenerative changes at an early stage of joint deseases, but also in monitoring the effectiveness of treatment.
Protein substances form the basis of all vital cell structures; they are a part of the cytoplasm and are the basis of all living things. The protein synthesis is a complex and multistage process. It is performed in special organelles called ribosomes. Violation of synthesis is possible at all the three stages: replication, transcription and translation. In medical practice, medicinal substances are used that can stimulate protein synthesis in the body.
The point was to decide the effect of blended editing of wheat with lentil on yield execution and to examine the best mix and proficiency of asset usage by deciding unrefined protein-land equal proportion. Blended trimming brought about diminished yields of both wheat and lentil when contrasted with sole editing. Further outcomes dependent on the examination of grain quality demonstrated that unrefined protein grouping of the blended yield wheat was expanded contrasted with wheat sole harvest, however was assessed lower than in sole and blended harvest grain lentil. The best on Nitrogen, unrefined protein focuses and protein LER in wheat grain was accomplished in blended trimming treatment T5: wheat (100 kg ha-1) + lentil (80 kg ha-1).
This research article investigates the association between dietary fiber, protein, and fat with manganese content in food. Manganese is an essential micronutrient involved in various physiological processes, and its dietary intake is crucial for maintaining optimal health. The study aimed to uncover the relationship between dietary fiber, protein, and fat and the manganese content in different food sources. Methods involved the analysis of a diverse range of food samples for their manganese content and determination of their dietary fiber, protein, and fat composition. Statistical analyses were performed to examine the associations. The results elucidate the significant influence of dietary fiber, protein, and fat on the manganese content in various foods. The findings provide valuable insights into the factors affecting manganese intake through dietary choices. Understanding this link can contribute to optimizing manganese intake and promoting overall health and well-being.
This research article investigates the association between dietary fiber, protein, and fat with manganese content in food. Manganese is an essential micronutrient involved in various physiological processes, and its dietary intake is crucial for maintaining optimal health. The study aimed to uncover the relationship between dietary fiber, protein, and fat and the manganese content in different food sources. Methods involved the analysis of a diverse range of food samples for their manganese content and determination of their dietary fiber, protein, and fat composition. Statistical analyses were performed to examine the associations. The results elucidate the significant influence of dietary fiber, protein, and fat on the manganese content in various foods. The findings provide valuable insights into the factors affecting manganese intake through dietary choices. Understanding this link can contribute to optimizing manganese intake and promoting overall health and well-being.
The methodological recommendation presents a comparative characteristic of surgical methods of treatment of deviation of the nasal septum. Deviation of the nasal septum occurs in the cartilaginous and bone regions, both separately and in both simultaneously. Deformations can be of different nature (bend, crest, spike) and localization. They are one and two-sided. Spines and ridges are more often localized at the junction of the quadrangular cartilage and the perpendicular plate, ploughshare, and the crest of the upper jaw (“growth zone”). Less often, the upper sections of the perpendicular plate and the rear section of the ploughshare are deformed. For traumatic deformities, characteristic bends with acute angles. Often, after injury, there is a displacement of the anterior edge of the quadrangular cartilage in the form of its subluxation. The deformation of the cartilaginous part of the external nose in patients under our supervision was caused by the deviation of the nasal septum. There were no patients with deformity of the nose who would have been indicated for surgical intervention on the cartilage of the external nose. When correcting the deformity of the external nose in patients, we used an exclusively closed technique of intervention with a transseptal access, and, if necessary, access under the upper lip, which allowed us to obtain a good cosmetic effect and minimize traumatic complications after surgery.
Biochemical studies of blood and chamber moisture in children with congenital cataract
are of great clinical importance. Chamber moisture is an ultrafiltrate of blood plasma and can be
an indicator of various pathological conditions of the body as a whole, and the eye in particular.
The study of the level of protein, glucose in the blood and chamber moisture of all the examined
children was carried out. As an indicator of inflammatory complications in the postoperative period,
the threshold value of total blood protein of 62.2±1.3 g/l and an increase protein content in chamber
moisture to 3.5±0.09 g/l can be used.
This article presents the results of a study of the hepatoprotective properties of the herbal drug darmonal in combination with phytin compounds in experimental animals (rats) with experimental hepatitis caused by SS14 intoxication. The obtained research results showed that darmonal in combination with phytin compounds increases the synthesis of total protein and normalizes the composition of protein fractions of blood serum. Both studied drugs have a high level of hepatotropic effect. The combination of darmonal and fitin-C was the most effective. According to this effect, they were superior to the hepatoprotective drug LIV-52.
Objective: to carry out comparative analysis of matrix metalloproteinase’s-2,9 (MMP-2, 9), tissue inhibitors of matrix metalloproteinase’s (TIMP) expression indexes and their interconnection with magnesium ions (Mg2+), glucosamine-glycans and glucuronidases activity in the blood of patients with undifferentiated connective tissue dysplasia. Material and research methods. 208 persons of them aged 15-25 (19.9±1.42) years have been studied. Depending on clinical signs and progressive complications, patients were divided into 2 groups, 1-group 97 patients with signs of external phenotypes (fens) of BTDD, 2-group 111 patients with signs of external and internal fens, with some complications. Results of researches have shown that the blood of patients with BTDD showed an increase in MMP-2 and MMP-9 and a decrease in TIMP-1. As the progression of the disease increases, the expression of MMP-2 and MMP-9 increases and the TIMP-1 decreases, compared to the data in the control group. At the same time, in patients there is a decrease in Mg 2ions in blood. Thus, dynamics of changes of MMP-2 and MMP-9 and also TIMP-1 expression in patients with undifferentiated connective tissue dysplasia may serve as a method of estimation of progressive pathological process and prognosis of the disease.
In this paper, we have studied one theorem on 2x2 Jordan blocks matrix. There are 4 important statements which is used for proving other theorems such as in the differensial equations. In proving these statements, we have used mathematic induction, norm of matrix, Taylor series of .
The object of research is the development of compound feed recipes from local oilseeds grown in Uzbekistan. The aim of this study is to introduce a recipe for the rational use of food purposes rich in proteins from local raw materials, as well as to study the physicochemical composition of acclimatized soybeans to increase the protein.
Rapid ramp up of immune responses against SARS-CoV-2 during pandemic enables adequate prevention and treatment for COVID-19. Estimating levels of SARS-CoV-2 spike protein antibodies post vaccination is crucial for designing mass-vaccination strategies. The aim of this study was to evaluate effectiveness of Sputnik V first dose in Pakistan. A cross-sectional study of 1000 participants was conducted for examining Gam-COVID-Vac or Sputnik V first dose effects at 21st days post administration at Islamabad Diagnostic Center, Islamabad, Pakistan. From 1000 real-time PCR negative (SARS-CoV-2 RNA) individuals, samples were collected and analyzed for antibodies to the SARS-CoV-2 spike protein using Electro-chemiluminescence immunoassay (ECLIA) (Elecsys # 09289267190 Roche, USA). 85% of the participants showed strong positive results with SARS-CoV-2 spike protein antibodies >1.5 AU/ml. The individuals with antibody titer >250 AU/ml were 34.9%. While participants with >100 AU/ml of antibodies were 12.7%. However 9.5% showed antibody titer of >25 AU/ml. 27% of participants had antibody titers of >1.5-2.5 AU/ml. While antibody titers of <1.5 AU/ml were observed among 15.9% of participants. Majority of the individuals represented significantly strong antibody titers against SARS-CoV-2 even before second booster dose of Ad5 based Sputnik V vaccine. Continuous monitoring of antibody levels among COVID-19 vaccinated populations are deemed to assess humoral immunity status against SARS-CoV-2 infections.
Higher convergences of fat and protein just as every one of the micronutrients were available in the stems of S. indica. Centralizations of alkaloid were higher in the leaves and stems of S. cayennensis where as more elevated level of protein was identified in the leaves. More noteworthy degree of hydrogen cyanide was found in the leaves of the two plants. Information were considered genuinely critical at P≤0.05. Nonetheless, high grouping of hydrogen cyanide, an intense poison, recognized in both the leaves and stems of the plants render them unpalatable without appropriate handling. In this way, the usage of these plants in ethno medication as food and medication could be deadly; consequently, it is exceptionally debilitate. Regardless, the bioactive mixtures and supplements could be removed for advancement of medications and food supplements.
Topicality and demand of the subject of dissertation. In the world lat days chanchcd structures of trauma, increase the number of heavy combined traumas, which resulting in more heavy nature of simultaneous injuries of three , four or more anatomical regions, which creates difficulties in determining of the order of care and surgical tactics in patients with combined traumas of the facial skeleton bones (CTFSB). The syndrome of mutual burdening injuries of various anatomical regions, variety, hcavity and speed of the development of pathological process did difficulty of diagnosis of the CTFSB. Complexity of the clinical picture, features of the progress of post-traumatic shock, the development of traumatic disease cause difficulties which arise in the course of examination of patients and put tasks to the experts to find new ways of developing diagnostic algorithms and early surgical treatment of the CTFSB.
Frequency of CTFSB ranges from 34,8 to 63,3%. Fractures of orbit has been observed with an extremely high frequency (98%) in CTFSB, injury of the orbit is accompanied by damage of the eyeball and its subsidiary bodies has been observed in 66 % of eases. Consequences of eye injuries arc becoming the leading cause of disability and in 50% of eases could cause permanent loss of vision. By reason of death combined trauma take the third part after coronary heart diseases. Frequency of disfiguring defects and deformities of face occurs in 12 and 57%, disability in CTFSB reaches up to 23%. CTFSB, combined with TBI, causes up to 60% of deaths.
The causes of unsufficient results is non-availability of a diagnostic algorithm, which includes the most informative research methods, determining the order of interaction and priority of work of doctors of various specialties in CTFSB.
In some eases, requires specified an indications, character, scope, sequence and timing of surgical interventions, depending of the objective assessment of heaviness of injuries to various anatomical regions, prognosis criteria, the nature and heaviness of life-threatening consequences of combined trauma. The research work earned out within the framework of the achievement of the set by the Decree of the President of Republic of Uzbekistan “About measures on the further deepening reform the health care system” November 28, 2011, № PD-1652, maintenance of high-quality medical aid to the population under modem requirements and standards.In this regard the need for the development of algorithms of diagnosis and early methods of surgical treatment of patients with CTFSB constitute one of the important criteria demand the theme of dissertation.
Purpose of research is improvement of the diagnostic tactics and therapeutic interventions in patients with acute combined injuries of the facial bones according to the severity and location of the injury.
Scientific novelty of disscrtational research consists in the following: revealed the structure and features provide consistent care to patients with combined injuries in Republic of Uzbekistan;
The sequence of diagnostic and therapeutic measures, depending on the patient's general condition with CTFSB first determined by using created CT program "ADIL
developed innovative methods for early reduction and fixation of bone fragments in CTFSB;
identified endogenous factors, affecting on the wound process, disclosed the mechanisms of post-traumatic complications in CTFSB;
proved, that at 2 - 3rd days after the injury occurs the depression of cell and humoral immunity in the blood. Increases the level of proinflammatory cytokines, reduced the level of anti-inflammatory cytokine (in 2,8 at patients with heavy commonl condition. Increased levels of pro - and reducing anti - inflammatory cytokines is a poor prognostic factor in the development of inflammatory complications (bone wound suppuration, osteomyelitis of the jaw bones, soft tissue abscess);
patients with CTFSB at 2 - 3rd days after the injury occurs the depression of the content of protein and micronutrients (calcium, potassium and phosphorus) in the blood, which is a prognostic factor of the development of complications;
a scheme was developed for integrated medical correction of endogenous factors affecting on the development of posttraumatic complications;
1. CTFSB in 100% of cases combined with TBI, in 27.7 % with injuries of skeleton and internal injuries. In the diagnosis and treatment of patients with CTFSB should participate resuscitator, maxillofacial surgeon, neurosurgeon, ophthalmologist, and otolaryngologist. Primary debridement of wounds, reduction and fixation of bone fragments in patients in compensated state should be done within 3 hours after injury, while at subcompensated state - during the first day, and at the decompensated state - within 3 days.
2. With the CT program "ADIL" can determine the overall condition of patients in a short time. The most informative diagnostic criteria arc the general condition of patients, level of consciousness, hemodynamic stability, shock index and temperature gradient. The severity of the general condition of patients is directly dependent on the localization of the fracture of the facial bones. Multiple fractures of the upper and middle areas of the face arc the most serious injury in patients.
3. Patients with CTFSB in compensated and subcompensated state emergency surgical aid and diagnostic procedures should be performed in full volume (maxillofacial surgery, traumatology, neurosurgery, surgery, ophthalmology and otorhinolaryngologist), including the reduction and fixation of bone fragments in the first day. To patients with CTFSB in state decompensated should be performed at least diagnostic procedures, limiting the amount of emergency surgery. Reduction and fixation of bone fragments should be done after the restoration of function of vital organs and systems.
4. The method of choice for the treatment of depressed large bone fragments of facial bones is a titanium distractor, the use of which gives a good clinical and functional outcome.
5. When depressed fracture of the zygomatic arch application of the developed device will allow us to produce reduction and fixation of bone fragments in the early stages (within one day) with a good cosmetic result.
6. At patients with CTFSB in posttraumatic period (7- 14th day.) there arc a deep depression of CD3, CD4 cell composition, humoral factors and secretory immune system, increased necrosis factor CD95, increasing the levels of proin-flammatory (IL-6 ) and a decrease - anti- inflammatory (IL -10) cytokines. On 9-10th day reduced total protein, calcium, potassium and phosphorus in the blood .
7. Reduction of cellular and humoral immunity, increased proinflammatory cytokine and tumor necrosis factor, reducing the anti-inflammatory cytokine , the protein concentration in the blood, calcium, potassium and phosphorus arc predictors of complications.
8. Application of complex drug therapy within the 1-3 days after the injury with the inclusion of immune ( immunomoduline, ribomunil ), enzyme ( Voben-zym ) drugs osteoplastic materials allows to correct the violation of homeostasis, also used to prevent complications.