ЕВРАЗИЙСКИЙ ВЕСТНИК ПЕДИАТРИИ 4(11) 2021
ISSN 2181-1954 EISSN 2181-1962
U DC 615.322:542.943-92'78
ANTIOXIDANT ACTIVITY OF A DECOCTION OF SANDY IMMORTAL FLOWERS AND
THE ROOT OF MALT AND REAL
KaldibaevaA.
0.
, Yiinusov A.
A.
Tashkent Pediatric Medical Institute. Uzbekistan.
Resume
The effect of a decoction of liquorice root and an infusion of Helichrysum flowers on the processes
of lipid peroxidation in chronic toxic hepatitis was studied. It has been established that a decoction of
liquorice root and an infusion of Sandy immortelle flower in chronic poisoning with heliotrin has an
antioxidant effect. In terms of hepatoprotective action, herbal preparations are not inferior to the well-
known hepatoprotector carsil.
Key words: decoction of liquorice root, infusion of Sandy immortelle flowers, chronic toxic
hepatitis, conjugated dienes, dienketones.
АНТИОКСИДАНТНАЯ АКТИВНОСТЬ ОТВАРА ИЗ ЦВЕТКОВ БЕССМЕРТНИКА
ПЕСЧАНОГО И КОРНЯ СОЛОДКИ И НАСТОИ
Калдибаева А.
О.,
Юнусов А. А.
Ташкентский педиатрический медицинский институт. Узбекистан.
Резюме
Изучено влияние настоя из цветков бессмертника песчаного и отвара из солодкового корня
на процессы перекисного окисления липидов при хроническом токсическом гепатите
Установлено, что отвар из солодкового корня и настой из цветков бессмертника песчаного
при хроническом отравлении гелиотрином оказывает антиоксидантное действие. По
гепатозащитному действию растительные препараты не уступают известному
гепатопротектору карсилу.
Ключевые слова: отвар из солодкового корня, пастой из цветков бессмертника песчаного,
хронический токсический гепатит, конъюгированные диены, диенкетопы.
ЎЛМАС ЎТ ГУЛЛАРИ ВА ЧУЧУКМИЯ ИЛДИЗИ ҚАЙНАТМАСИ
ДАМЛАМАСИНИНГ АНТИОКСИДАНТ ТАЪСИРИ
Калдибаева А.О., Юнусов А.А.
Тошкент Педиатрия Тиббиёт Института. Узбекистан.
Резюме
Улмас ўт гули дамламаси ва чучукмия илдизи цайиатмасинипг сурункали токсик гепатитда
ёглар переоксидланит ззсараёнига таъсири ургапилгаи. Олинган тазкрибаларга кура, чучукмия
илдизи цайнатмаси ва ўлмас ўт гули дамламаси гелиотрин билан чацирилган сурункали
заҳарланишда оксидланишга царит таъсирга зга эканлиги аникранди. Шифобахуи
ўсимликлардан тайёрланган цайнатма ва дамлама зкигарни ҳимоя цилиш таъсири бўйича,
гепатопротектор карсил препаратидан цолишмаслиги келтирилган.
Калитли сўзлар: чучукмия илдизи қайнатмаси, ўлмае ут гули дамламаси, сурункали токсик
гепатит, коиъюгирланган диенлар, диенкетонлар.
Introduction
It is known that herbal medicines have been used
for the treatment of chronic viral hepatitis and other
liver diseases for more than 30 years, and practical
medicine has a fairly wide arsenal of various
antioxidant medicines. However, when using synthetic
drugs, various side effects and complications are often
observed, which largely limit their successful use in the
clinic.
All this necessitates the search and study of new
highly active substances, especially on tire
28
ЕВРОСИЁ ПЕДИАТРИЯ АХБОРОТНОМАСИ 4(11) 2021
ISSN 2181-1954. EISSN 2181-1962
basis
of herbal remedies, which, in comparison with
synthetic ones, have low toxicity. In this regard,
compounds isolated from the liquorice root
are
of
great
interest. Interest in
liquorice
as an extraordinary and
multifaceted
plant
persists in our time, since liquorice
root preparations are successfully used for various
diseases [7]. The main medicinal properties of
liquorice root are mainly due to glycyrrhizic acid acid
and glyceretic acid aglycone. These glycosides and
their various derivatives have a wide spectrum of
biological
activity.
They
possess
estrogenic, anti-
inflammatory
[5],
anti-ulcer [2], antiallergic
[14],
antitoxic and hepatoprotective [8] effects and exhibit
antiviral activity [6]. The introduction into practice of
new drugs based on liquorice root, is promising due to
their low toxicity and
the
absence of unwanted side
effects. Sandy immortelle is a perennial herb 30-40 cm
high, belongs to
the
Asteraceae family. Flavonoids and
flavonoglycosides
are found
in
flowers. Flowers have
choleretic properties. They are used for liver diseases,
cholecystitis, and hepatocholecystitis
Several plant extracts used to treat gastrointestinal
and biliary disorders contain hepatotoxic alkaloids and
are more harmful than beneficial. However, a number
of extracts contain substances that have antioxidant,
anti-
fibrotic,
antiviral
and anticarcinogenic properties.
One of these herbal remedies is glycyrrhizin, a
triterpene saponin that is part of an aqueous extract of
liquorice
root. Therefore,
the
available
data
demonstrate the positive effect of glycyrrhizin in
chronic viral liver diseases and its hepatoprotective
properties.
However,
it
is rather difficult
to
judge the
effectiveness of glycyrrhizin
in
the treatment of
chronic viral hepatitis, given the virtual absence of
randomized placebo-controlled clinical trials.
Despite this, glycyrrhizin certainly deserves
attention and further research.
The aim of this work is to study the effect of
an
infusion
of
immortelle flowers and a
decoction of
licorice root on the products of lipid peroxidation and
the activity of enzymes of the antioxidant system in
chronic toxic hepatitis.
Materials and methods
The experiments were carried out on 30 male rats
weighing 150-180
g.
Salme was injected into intact
groups of animals. In the remaining groups of animals,
chronic toxic hepatitis was induced by subcutaneous
administration of heliotrin
at
a dose
of
10 mg
/ 100g
during the
first week
(Abdullaev N.K. et al. 1989).
During the second week, his dose was reduced to
7.5
mg
/
100g, and in the subsequent third week, to 5
mg /
100g. For prophylactic purposes, rats in the control and
experimental groups for one month orally through a
probe in a volume of 1 ml
/
100 g were injected with a
decoction of liquorice root (first group), an infusion of
immortelle flowers (second group) at a concentration
of 1:10, and hepatoprotector was also administered
orally, carsil at a dose of 100 mg
/
kg for one month
(third group). The parameters in rats with chronic toxic
hepatitis served as a control.
In the liver homogenate, the content of diene
conjugates (Shilina N.K. et al. 1978), malonic
dialdehyde using thiobarbituric acid (Krichevskaya
A.A. et al. 1976), as well as the activity of the main
enzymes of the antioxidant system, superoxide
dismutase (SOD) and catalase were determined. (CT)
(Gurevich V.S. et al. 1990; Korolyuk M.A. et al. 1988).
Statistical processing of the data obtained was carried
out according to the method of Strelkov R.B. (1986),
Results and discussion
In animals with chronic toxic hepatitis, general
weakness, disheveledness and a decrease in coat gloss,
abdominal distension, and ascites were noted. Death
occurs in 40% of cases. Under the influence of
hepatotoxin-heliotrin, along with the disturbance of the
general condition in rats, the content of diene
conjugates and malonic dialdehyde increases in the
liver tissue. Simultaneously with the accumulation of
lipid peroxidation products, the activity of SOD and
CT decreases by 64% and 42%, respectively, in
comparison with similar parameters in intact animals.
A decrease in the activity of enzymes of the antioxidant
system leads to the formation of
a
superoxide anion.
This radical, reacting with hydrogen peroxide,
increases the formation of reactive oxygen and
hydrophilic radicals, causing destruction of hepatocyte
biomembranes and the development of protein and
fatty degeneration of liver cells [3]. These data indicate
that heliotrin, like other hepatotoxins, has a prooxidant
effect, leading to disruption of metabolic processes in
the
liver.
The simultaneous
use
of medicinal plants with
heliotrin leads to a decrease in the disorders of the
antioxidant system and inhibition of the enhancement
of lipid peroxidation. As a result of the introduction
during the month of a decoction of liquorice root and
an infusion of sandy immortelle flowers, the content of
conjugated dienes decreases by 22% and 25%,
respectively, conjugated dienketones
-
by 32%,
malonic dialdehyde by 34% and 35% Under the
influence of carsil, the activity of these enzymes is
increased by 71% and 36%.
29
ЕВРАЗИЙСКИЙ ВЕСТНИК ПЕДИАТРИИ 4(11) 2021
ISSN 2181-1954 EISSN 2181-1962
If we take this effect into account, then liquorice
root and flowers of sandy immortelle, similarly to
silibor, have an antioxidant effect and prevent an
increase in the intensity of lipid peroxidation. (Table
1.)
The antioxidant effect of drug plants is apparently
associated with an increase in the activity of enzymes
of the antioxidant system, since the composition of
liquorice root contains glycyrrhizic acid (8-24%),
flavonoids (4.3%) and organic acids (4.6%) [2]. The
medicinal properties of liquorice root re mainly due to
the content of glycyn’hizic and aglyconglyceretic acid.
These glycosides and their various derivatives have a
wide spectrum of biological activity.
Most of these substances have direct and indirect
antioxidant effects. Probably, these substances lead to
an increase in the activity of enzymes and an increase
in the function of the antioxidant system of the div.
In addition, the studied medicinal plants have anti-
inflammatory,
choleretic,
immunomodulatory
properties and increase the regenerative potential of
liver cells (Saratikov A.S. etal. 1991).
Table №1. Influence of liquorice root and immortelle flowers on lipid peroxidation indicators. M ± m (p
= 0.05)
Experimental
conditions
Conjugated
dienes, D
/
1
mg
lipid
Conjugated
dienketones,
D
/1 mg
MDA lipid,
nmol
/
1 g
tissue
SOD,
conventional
units
/
min mg
protein
Catalase, nmol /
min mg protein
Intac group
0,45040,023
0,22440,014
36,141,01
J ,4540,08
0,4340,016
Control
group
(chronic hepatitis
caused by heliatrin)
0,73640,0217
0,39740,028
71,341,14
0,52±0,042
0,2540,019
decoction
from
liquorice root
0,57240,028
0,26540,024
47,241,03
0,8740,037
0,3440,019
Infusion of sandy
immortelle flowers
0,55040,016
0,26840,015
46,740,94
0,8740,063
0,3340,013
Carsil
0,56040,024
0,25440,021
46,841,74
0,8940,07
0,3440,02
The antioxidant activity of carsil is less expressed.
The results obtained indicate that these natural
substances have a unidirectional antioxidant effect.
Glycyrrhizin is a calcium and potassium salt of the
three basic glycyrrhizic acid, aglycone, which is
glycyrrhizic (glycyretic) acid. Glycyrrhizin has anti-
inflammatory,
antiallergic,
antiviral,
mineralocorticoid,
antiulcer,
hepatotropic
and
immunomodulatory effects. Of particular interest is its
antiviral and immune effects.
It is known that glycyrrhizin stimulates the
formation of interferon gamma, which in turn activates
macrophages and В cells, enhancing phagocytosis and
antidiv production, and activates NK cells. In a
number of works, there are indications of the inhibitory
effect of glycyrrhizic acid directly on the DNA and
RNA of viruses, which causes the inactivation of viral
particles, their suppression, and their introduction
through the membrane into the cell, a violation of the
synthesis of new structural components of viruses.
An important property of glycyrrhizin, which partly
explains its positive effect on the course of chronic
hepatitis, is the ability to suppress the formation of free
radicals and the production of lipid peroxides.
It is known that in chronic liver diseases of various
origins, the activity of antioxidant defense enzymes
decreases, which is associated with the activation of
lipid peroxidation processes. The course and severity
of damage to hepatocyte membranes depends on the
enhancement of lipid peroxidation and is accompanied
by enzymatic deficiency, impaired cell division,
leading to their death. For the treatment of chronic
hepatitis, various drugs that have a hepatoprotective
effect are used (11). For example, such drugs as
Essentiale, Essel Forte, Phosphagliv, Apcosul,
Herbion, Legalon, Liv-52,
ЕВРАЗИЙСКИЙ ВЕСТНИК ПЕДИАТРИИ 4(11) 2021
Holagol, Carsil, Hofitol,
Silibor, etc. However,
the
arsenal of these drugs
is
small and does not always
satisfy the needs of clinicians. Some hepatoprotective
agents cause side effects of varying
degrees; in
addition, they
are all
imported. In this
regard,
tire
pharmacotherapy of chronic liver
diseases
should be
complex
and
help correct multifactorial changes in
various links of pathogenesis.
Conclusion
1.
In chronic toxic hepatitis caused by heliotrin,
there is an increase in the processes of lipid
peroxidation and a decrease in the activity of specific
enzymes SOD and KT.
2.
Preventive administration
of
medicinal plants -
liquorice root, sandy immortelle leads to inhibition of
lipid peroxidation processes and activation of
antioxidant enzymes of the div.
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Entered 09.12.2021
