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PATHOLOGICAL ANATOMICAL CHANGES AND THE RESULTS OF
HISTOLOGICAL EXAMINATION IN RABBIT COLIBACILLOSIS
Sultanova I.Yu
PhD Researcher Scientific Research Veterinary Institute of Uzbekistan.
Dzhuraev O.A
Head of the Laboratory of Pathological Anatomy
Аnnotation
The article presents the features of the transformation of clinical signs
postoanotomic morphological changes and the manifestation of a peculiar
appearance.
Keywords:
histocut, dystrophy, necrosis, karyolysis, infiltration, point blood
outpouring, heart attack.
Аннотация
Ма
қ
олада
қ
уёнлар колибактериоз касаллигининг клиник белгилари, кечиш
хусусиятлари, патологоанатомик ва морфологик ўзгаришлар
ҳ
амда
гистокесмалардаги ўзига хос кўринишларнинг кузатилиши ёритилган.
Аннотация
В
статье
приведены
особенности
клинических
признаков,
патологоанатомических
и
морфологических
изменений
а
также
своеобразность гистологической картины при данном заболевании.
Ключевые слова:
гистосрез, дистрофия, некроз, кариолизис,
инфильтрация, точечное излияние крови, инфаркт левого предсердия.
Relevance of the Topic
Currently, there is a growing interest in the development of rabbit breeding among
farms and the population of the Republic of Uzbekistan. At the same time, the
increase in the number of rabbits under care, and the presence of large numbers of
these animals in a limited area, in turn, cause some serious problems. Violation of
veterinary and sanitary rules when keeping and feeding rabbits leads to various
infectious and non-infectious diseases. One of these diseases - colibacillosis in
rabbits - remains one of the most pressing problems.
Rabbit colibacillosis is an acute infectious disease caused by E. Coli, a shorter and
wider bacterium with a rounded tip (0.2-0.7 x 2-4 microns), rod-shaped, gram-
negative, without spores and capsules. The disease is accompanied by severe
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intoxication and diarrhea, which occurs in rabbits for 1-4 days, usually up to 5-10
days (A.I. Yatusevich and others. 2008). Currently, rabbit colibacillosis occurs in
rabbits aged 5 to 14 days in the local conditions of the country.
Deficiencies in storage and care lead to a decrease in the resistance of rabbits and
their offspring, a deficiency of vitamins A and B and metabolic disorders, which leads
to a secondary disease, that is, the causative agents of colibacillosis enter the div
through food, water, dishes, feces of birds and rodents.
Materials and Methods
The pathological samples brought from the districts of the Samarkand region to the
laboratory for the study of diseases and pathological anatomy of young cattle of the
Research Institute of Veterinary Medicine have been investigated. According to the
history, rabbits aged 10-14 days were infected with colibacillosis in the form of
enteritis (Figure 1), with yellowish-gray liquid diarrhea, weight loss, loss of appetite,
tremors and death within 3-5 days. Изменения наблюдались в основном в
желудочно-кишечном тракте при патологическом вскрытии кроликов. The
presence of a viscous mass in the stomach, hemorrhagic inflammation of the mucous
membrane, punctate bleeding (Figure 2), infiltration of the small intestine,
hemorrhagic catarrhal enteritis of the mucous membranes, the formation of foci of
necrosis were observed. Enlarged spleen and liver, pinpoint hemorrhages on the
surface, choking with blood, nephritis in the kidneys, pinpoint bleeding, bladder
filling with urine, protein deposits in urine, left ventricular infarction (Figure 3) and
general toxicosis.
Figure 1. Carcasses of rabbits up to 10-14 days Figure 2. Hemorrhagic
inflammation of the of age infected with colibacillosis. gastric mucosa, punctate
hemorrhage.
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Figure 3. Left ventricular infarction.
Research Results
All pathological samples from internal organs were fixed in 10% formalin,
histological incisions made after anesthesia, hardening, paraffinization were
examined under a microscope, the following morphological changes characteristic
of intoxication under the influence of E. Coli were observed in rabbits.
Decomposition of hepatocytes in the liver, endovasculitis, perivascular edema,
swelling of fibrin filaments, infiltration of lymphocytes, hemostasis in the vessels
develop. Hemolysis bodies were observed around the central veins, and hemolysis
was observed in the cytoplasm of perivascular hepatocytes. (Figure 4)
Figure 4. Endo- and perivasculitis, infiltration of vascular and hepatic barriers,
hepatocyte necrosis, karyolysis.
Infiltrative inflammation in a special fundic part of the gastric mucosa is
accompanied by a violation of the structure of epithelial cells that form the wall of
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the fundic glands, that is, lysis of the nuclei. Interstitial argyrophilic cells in the
pyloric part of the stomach developed degeneration and necrosis (Figure 5).
Figure 5. Necrosis of intermediate argyrophilic cells of the pylorus of the stomach.
Cellular dystrophy. Vascular infiltration.
There are necrotic changes in the mucous membrane of the small intestine,
desquamation of cells, naked lamellar plaque (Figure 6). In addition to
hypersecretion and hyperplasia of the glands of the mucous membrane, infiltration
of tissue elements (lymphocytes, neutrophils, leukocytes, plasmablasts, fibrocytes,
fibroblasts) also develops in the layer. Hyperplasia, necrosis and desquamation of
lymphoid follicles were observed. Focal necrotic changes were also observed in
muscle fibers and serous membranes. (Figure 6)
Figure 6. Necrosis and desquamation of the mucous membrane of the villi of the
small intestine.
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Thinning of lymphatic follicles, atrophy, edema of interstitial connective tissue,
desquamation of vascular endothelial cells, perivascular edema, follicular
necrobiosis are formed in the spleen. The connective tissue is expressed in the form
of fibers, thinning of lymphocytes in the lymphatic follicles, and in some necrotic
foci of the follicular lymphocytic space, pycnosis of the nucleus, hemorrhage under
the capsule were revealed (Figure 7).
Figure 7. In the foci of spleen necrosis - the cavity of follicular lymphocytes,
pycnosis of the nucleus, hemorrhage under the capsule.
Сonclusions
Observations and studies have shown that colibacillosis is widespread in Uzbekistan,
especially among young rabbits aged 10-14 days and is an acute disease that leads to
the development of complex and irreversible pathological, anatomical and
morphological processes in the div, which in turn leads to fatal the exodus of
animals.
References
1.
M.S. Abdullakhoyaeva. "Pathological Anatomy". Tashkent, 2012.
2.
Annikov V.V., Krasnikov A.V., Korotova D.M. Short course of lectures “Diseases
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Professional Education. "Saratov State Agrarian University", 2017.
3.
Zufarov K.A. other. "A practical guide to histology" 1976.
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Ibodullaev F. "Pathological anatomy of farm animals." Tashkent, "Uzbekistan".
2000.
5.
S.V. Leontyuk and others. "Diseases of rabbits." Moscow. "Kolos". 1974.
6.
G.A. Merkulov "Course of pathological and histological techniques" Leningrad,
"Medgiz". 1973.
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7.
M.K. Nedzved., E. D. CHyorstviy. "Pathological Anatomy" 2011.
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Elmurodov B.A., Turdiev A.K., Nabieva N. "Rabbit breeding". Samarkand. 2018.
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Yatusevich A.I. "Contagious diseases of fur animals". Monograph. Vitebsk, 2008.
