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Volume 7 | May 2023
ISSN: 2795-5621 Available: http://procedia.online/index.php/applied/index
69
ETIS-2 COMPLEX DRUG AND SALMONELLA OF OTHER TYPES OF
ANTIBIOTICS INFLUENCE ON DRIVERS
Djurakulov O. K.
Basic doctoral student, Veterinary Scientific Research Institute
Navruzov N. I.
V.f.f.d., junior researcher, Veterinary Scientific Research Institute
Mamadullaev G. Kh.
V.f.d., scientific supervisor, Veterinary Scientific Research Institute
Annotation:
This article provides information that the indicators of sensitivity to antibiotics in
relation to aqueous and oil emulsions of various concentrations of the drug ETIS-2 to the causative
agent of salmonella enteritidis were carried out in laboratory conditions.
Keywords
:
S.Enteritidis, S.Typhimurium, S.Dublin, antibiotic, strain, GPA (meat peptone
agar) and GPQ (meat peptone broth), ETIS-2 complex preparation, susceptibility, resistance, pathogen,
concentration, ratio, dilution.
Introduction.
Currently, salmonellosis is one of the important problems not only in our republic, but
also in the veterinary and medical fields worldwide. Salmonella Enteritidis, Salmonella Typhimurium
and Salmonella Dublin pathogens are often found among farm animals and cause damage to livestock.
It is scientifically known that these Salmonella serovars cause foodborne outbreaks in animals and
humans [1]. For the prevention and treatment of salmonellosis, sulfanilamide, nitrofuran preparations
and antibiotics of various groups are used. The complex drug "ETIS-2" consisting of pharmacopoeia
drugs was developed by the scientists of VITI Tuberculosis Research Laboratory against diseases of
bacterial etiology. If the ETIS-2 complex drug is used, there is no need to use other types of antibiotics
or other treatment agents [12]. The goal of our research is to determine the sensitivity and resistance of
different concentrations of antibiotics and ETIS-2 not only to salmonella species, but also to other
types of bacteria. The purpose of the study is to study the effect levels of the ETIS-2 drug and some
antibiotics on Salmonella Enteritidis pathogens, to compare the effectiveness of these drugs in the
treatment of salmonellosis, and to analyze the hematological changes in the div of treated sheep.
Methods
. Salmonellosis is an acute infectious disease of young animals in septic form, characterized
by fever, gastrointestinal disturbances, and diarrhea. Calves are infected with salmonellosis mainly
from 3-4 weeks to 4 months, piglets to 4 months, sheep at any age, poultry in the first days of life, and
mares are infected with specific pathogens (S. pullorum, S. gallinarum) in the mother's womb [5].
The causative agent belongs to the salmonella group, polymorphism is clearly expressed in the
microbial cell, it is well stained with aniline dyes. Good growth in GPA and GPQ. Biochemically, it
does not form indole, releases hydrogen sulfide, does not curdle milk. It remains unchanged in lactose
and sucrose. Glucose, mannitol, maltose form gas and acid. The microorganism produces a strong
poison (endotoxin) from itself. It shows different sensitivity and resistance to antibiotics and
sulfonamides[6]. In case of disease, animals are recommended to be divided into the following groups
after clinical examination and thermometry:
Procedia of Theoretical and Applied Sciences
Volume 7 | May 2023
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70
1)
healthy;
2)
a disease is suspected;
3)
clearly infected;
4)
animals that have recovered from illness.
Each group should have separate feeders-shepherds and equipment. It will be necessary to establish
nutritious and high-quality feeding. Levomycetin, syntomycin, tribrissen drugs are recommended for
treatment. When a complication of pneumonia is observed, it gives a good treatment result when it is
used in combination with antibiotics, sulfanilamides (norsulfazol, disulfan, etazol, sulfadine,
sulfademyzin) [6]. Antitoxin or hyperimmune blood serums used against salmonellosis also give very
effective therapeutic results. Always determining the sensitivity of pathogens to various drugs
increases the effectiveness of treatment measures. This task is assigned to laboratories working with
infectious diseases [7].
Discussion
. In order to recommend antibiotics to sick animals, there should be a number of clinical
signs, including the appearance of purulent sputum, changes in blood analysis, that is, an increase in
the number of leukocytes and neutrophils, indicators of inflammation, an increase in the amount of C-
reactive protein. Bacteriological cultures are grown from div fluids and the results are analyzed.
Depending on the type of antibiotic that is effective against the infection, the causative colonies that
grow in this process can be recommended one of the antibiotics. In this period, the wrong or mixed
antibiotics have an adverse effect on the liver, kidneys and intestines, not on the microorganisms, and
lead to various complications [8]. With the help of the complex drug "ETIS-2" composed of
pharmacopoeia drugs against diseases of bacterial etiology, many farms in the republic were cured of
tuberculosis and the expected economic damage was prevented, as well as the risk of the disease being
transmitted to people was eliminated [11]. "ETIS-2" is a combination of the components included in
the complex drug, which creates its advantage over other bacteriostatic drugs. Such a combination has
a synergistic effect (enhancement of the effect of one drug by another) and prolongation (extending the
duration of the effect of the drug). The drug can be used on all types of animals, regardless of their
physiological state, from the age of 10 days [12].
1)
(No. 1) Fat emulsion of ETIS-2 drug in a ratio of 1:10 - sensitive.
2)
(No. 2) 1:100 aqueous solution of the ETIS-2 drug is less sensitive.
3)
(№2) 1:100 oil emulsion of the ETIS-2 drug is sensitive.
4)
(No. 3) 1:50 ratio of ETIS-2 drug (aqueous) - less sensitive.
5)
(No. 3) 1:50 ratio of ETIS-2 drug (oily) – less sensitive.
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6)
The main concentration of ETIS-2 drug is very sensitive.
7)
(No. 1 water) 1:10 aqueous solution of ETIS-2 preparation - not sensitive.
8)
(No. 2 oil) Ot Ditrim 1:10 oil solution - not sensitive.
9)
(No. 4 water) Nitox 1:10 solution - not sensitive.
10)
(No. 5 water) Enroflan 1:10 solution - sensitive.
Antibacterial drug discs were incubated at +37°C for 24 hours. According to the categories of
sensitivity to antibiotics (susceptible if the growing area of the bacteria on the disc is up to 1.5-2.5 cm,
less sensitive up to 1.5 cm and not sensitive if there is no non-growing area of the bacteria) was
determined by comparing the growth zone for each colony [3 ].
RESEARCH RESULTS.
In order to determine antibiotic sensitivity by disk diffusion method, ETIS 2 drug, Ditrim, Nitox and
Enroflan antibiotics diluted water and oil emulsions were used in our studies. They were found to be
susceptible to S. enteridis strain (Table 2).
Susceptibility to ETIS-2 drug and other antibiotics.
N
Name of
the drug
Dilution
rate
The effect
According to the
susceptibility of
Salmonella
pathogens grown
on Bismuth
Sulphite Agar
According to the
susceptibility of
Salmonella
Shigella to
Salmonella
pathogens grown
in food
Blood agar base
(Infusion agar
base) according to
the sensitivity of
Salmonella
pathogens grown in
the food medium
1
ETIS-2
(oil
emulsion
)
1:1
Sensitive
Sensitive
Sensitive
1:10
Sensitive
Less sensitive
Less sensitive
1:50
Less sensitive
Less sensitive
Less sensitive
1:100
Not sensitive
Less sensitive
Less sensitive
2
Ditrim
1:10
Sensitive
Sensitive
Sensitive
1:50
Less sensitive
Less sensitive
Less sensitive
3
Nitoks
1:10
Less sensitive
Less sensitive
Sensitive
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1:50
Not sensitive
Not sensitive
Less sensitive
4
Enroflan
1:10
Sensitive
Sensitive
Sensitive
1:50
Less sensitive
Less sensitive
Less sensitive
According to the results of Table 2, according to the sensitivity of the strain of S. enteridis grown in
the food medium with Bismuth Sulphite Agar: the salmonella pathogen is sensitive to the oil emulsion
of the ETIS-2 complex preparation in the ratio of 1:1 and 1:10, 1:50 emulsion is less sensitive and
1:100 oil emulsion was found to be insensitive. It was found that the 1:10 oil emulsion of Ditrim drug
is sensitive to salmonella, and the 1:50 emulsion is less sensitive. It was found that the 1:10 oil
emulsion of the drug Nitox is less sensitive to salmonella, and the 1:50 emulsion is not sensitive. It was
found that Enroflan preparation 1:10 oil emulsion is sensitive to salmonella, 1:50 emulsion is less
sensitive.
Conclusion.
1.
Water and oil emulsions of the ETIS-2 complex drug were found to have a bactericidal and
bacteriostatic effect on Salmonella Shigella, Bismuth Sulphite and Blood agar base (Infusion agar
base) agars by disc diffusion method.
2.
Salmonella pathogen is sensitive to the oil emulsion of ETIS-2 complex drug in the ratio of 1:1 and
1:10. Oil emulsion in the ratio of 1:50 and 1:100 is less sensitive to salmonella.
3.
As a result of hematological hematological examinations in sheep treated with ETIS-2, after the
animals of experimental group I-II were infected with Salmonella agents, when the clinical
symptoms of the disease began to appear, it was found that the amount of hemoglobin,
erythrocytes and leukocytes decreased, the amount of lymphocytes increased, and hematocrit
decreased. These indicators caused signs of anemia in the sheep.
4.
If the ETIS-2 complex drug is used in the treatment of salmonellosis, there is no need to use other
types of antibiotics or other therapeutic agents.
5.
The mutual combination of the components included in the ETIS-2 complex drug - created its
advantage over other bacteriostatics. Such a combination has a synergistic effect (enhancement of
the effect of one drug by another) and prolongation (extending the duration of the effect of the
drug). The drug can be used on all types of animals, regardless of their physiological state, from
the age of 10 days.
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