The purpose of the study is to study the sensitivity of microbes to certain drugs in vitro! Material and Methods: to establish this research method, we have prepared 18-hour cultures of microbes - taking into account the most frequent inhabitants in the oral cavity. 1-2 ml of the studied microbes were applied to the surface of the dried nutrient medium by Mueller Hinton. The preparation sea buckthorn oil had an antibacterial effect on most microbes, although among them the most sensitive were such microbes as streptococci, proteas and pseudomonas. At the same time, the chlorhexidine drug had an effect on most microbes, but the effect was insignificant and only streptococci were the most sensitive and amounted to 18.0 ± 0.3 mm. Conclusions: the drugs used to treat sick children belonging to the group of traditional therapy have a weak antibacterial effect. At the same time, drugs from the special treatment group have a more pronounced antibacterial effect, especially on microbes of the genus streptococci.
In vitro evaluation of enzyme supplementation is used to predict the in vivo response of poultry to enzymes. This study aims to evaluate the efficacy of in vitro techniques in predicting the in vivo response of poultry to enzyme supplementation. Different in vitro techniques such as pepsin, amylase, and protease assays were used to evaluate enzyme efficacy. The results obtained from in vitro techniques were compared with the in vivo response of poultry to the same enzyme supplementation. The study showed that in vitro techniques such as pepsin, amylase, and protease assays can be effectively used to predict the in vivo response of poultry to enzyme supplementation. The study recommends the use of in vitro techniques for predicting the in vivo response of poultry to enzyme supplementation.
The aim of this study was to evaluate the antifungal property of Parangi Rasayanam, a Siddha drug, through in vitro screening. Parangi Rasayanam is a traditional herbal formulation used in Siddha medicine, a system of traditional medicine practiced in South India. Fungal infections pose a significant health burden, and alternative treatments are sought to combat drug-resistant strains. In this study, Parangi Rasayanam was subjected to in vitro testing against a panel of clinically relevant fungal strains. The findings demonstrate the potential antifungal activity of Parangi Rasayanam and provide a basis for further investigation and development of this Siddha drug as a therapeutic agent. Parangi Rasayanam is a traditional Siddha drug formulation used in South India, known for its medicinal properties. In this study, we aimed to evaluate the antifungal property of Parangi Rasayanam through in vitro screening against clinically relevant fungal strains. Standard methods such as agar disk diffusion and broth microdilution assays were employed to assess the inhibitory activity of Parangi Rasayanam. Our findings demonstrated significant antifungal activity of Parangi Rasayanam against a range of fungal strains, including Candida albicans, Aspergillus fumigatus, and Trichophyton rubrum. These results support the traditional claims of Parangi Rasayanam's antifungal potential and highlight its potential as an alternative treatment for fungal infections. Further studies are warranted to elucidate the active constituents and mechanisms of action of Parangi Rasayanam and to assess its efficacy and safety in clinical settings. The development of Parangi Rasayanam as an antifungal therapeutic agent holds promise in combating drug-resistant fungal infections.
Genetik rekombinatsiya — ikki xromosomalararo genlarning almashinuvidir. Virus va bakteriyalarda genetik rekombinatsiya hayvonlarga nisbatan kamroq bo'ladi. In vitro sharoitidagenetik rekombinatsiyani amalga oshirishning mohiyati turli organizmlardan DNKni ajratish, DNKning gibrid molekulalarini olish va hosil bo'lgan rekombinant molekulalarni o'ziga xos oqsilning sintezini hosil qilish maqsadida tirik hujayralarga kiritishdan iboratdir.
The article describes the technology of micro-clonal propagation of lemon Meyer variety in vitro. In vitro, the stages of sterilization, culture, branching, rooting of lemons in the propagation of the Meyer variety of lemon were studied. Different ratios of MS nutrient medium and different concentrations of auxin and cytokinin to the development of lemon exclusions were elucidated.
O’simliklarni yetishtirishning In vitro usulda o’simliklarni yetishtirishning samarali , sermahsul, biroz qimmat ammo foydali , tez o’sishtirish usuli ko’rib chiqildi. O’rganish natijasida in vitro usulida ekish o’simlikni zararkunandalarsiz va organik moddalarga boy holda olish imkoni mavjudligi aniqlandi.
Melaninlar azot o'z ichiga olgan va azotsiz polifenollarning fermentativ oksidlanishi paytida organizmlarda hosil bo'lgan quyuq rangli yuqori molekulyar tartibsiz polimerlardir. Ular radioprotektiv va antioksidant xususiyatlarga ega, radionuklidlar va og'ir metallar uchun sorbent bo'lib, tirik organizmlarni ultrabinafsha nurlanishidan samarali himoya qilishga ega. Melaninlar tibbiyot, farmakologiya, qishloq xo'jaligi va boshqa sohalarda qo'llaniladi [1].
По данным Всемирной Организации Здравоохранения 2,5 % из новорожденных имеют различные пороки развития. 1,5% из них проявляются под действием неблагоприятных экзогенных факторов. Хромосомные болезни человека - одна из наиболее распространённых форм наследственной патологии, которая встречается с частотой 7-3 случаев на 1000 рождений.
The present study aims to formulate and evaluate an herbal skin cream with wound healing activity. Herbal remedies have long been utilized for their potential in promoting tissue repair and wound closure. In this research, a skin cream was developed using a combination of herbal extracts known for their wound healing properties. The formulated cream was subjected to a series of in vitro and in vivo evaluations to assess its efficacy in wound healing. In vitro assays included cell proliferation, collagen synthesis, and antimicrobial activity, while in vivo studies involved excision and incision wound models in animals. The results demonstrated that the herbal skin cream significantly enhanced cell proliferation, collagen production, and exhibited antimicrobial properties. Moreover, the in vivo models exhibited accelerated wound healing, reduced scar formation, and improved tissue regeneration compared to control groups. This study presents a promising formulation of an herbal skin cream with potent wound healing properties, underscoring the potential of natural remedies in advancing wound care treatments.