The purpose of the study was to determine and evaluate the features of morphological changes in the liver parenchyma of 5-month-old white outbred rats under the influence of anti inflammatory drugs under conditions of polypharmacy.
The object of study for experimental studies was taken 250 white male rats weighing 200-250 g.
The scientific novelty of the research is as follows: polypharmacy of anti-inflammatory drugs negatively affects all parameters of liver structures. Under the influence of polypharmacy, there is a decrease in the absolute mass of the liver, volume and morphological parameters of the liver parenchyma. The decrease in morphometric parameters depends on the number of drugs in polypharmacy; under conditions of polypharmacy, the state of the hepatic capillaries and internal bile ducts, as well as biological membranes, was studied, as a result of which the structural structure of the liver, the development of destruction of the hepatic tissue were studied, and the morphofunctional foundations of this condition were shown.
Implementation of the research results. Based on the obtained scientific results, the morphofunctional characteristics of the liver of rats in the norm and under the influence of polypharmacy were determined:
Approved methodological recommendations: "Methodology for determining the morphometric parameters of the liver during polypharmacy of anti-inflammatory drugs" (Conclusion No. 8n-r / 265 dated 14.03.2022 of the Ministry of Health of the Republic of Uzbekistan) and "Methodology for determining the morphometric parameters of the liver during polypharmacy of anti-inflammatory drugs" (Uzbekistan, Conclusion of the Ministry of Health of the Republic of Uzbekistan No. 8 n-z 180 of 2022), electronic program No. DGU 1038 "Program for studying the comparative characteristics of morphological changes caused by polypharmacy in the liver."
The scientific results obtained in the study of morphological and functional properties and morphometric changes in the structure of the liver under the influence of polypharmacy have been introduced into the practice of the Samarkand branch of the Republican Specialized Oncological and Radiological Scientific and Practical Medical Center of the Samarkand City Medical Association (Order of the Ministry of Health of the Republic of Uzbekistan dated March 14, 2022, 8n-r / 265-No. and conclusion No. 8 n-z 180 of 2022). The implementation of the obtained research data allows developing methods for early diagnosis, treatment and prediction of organopathology by morphological parameters, improving the quality of life and reducing the number of complications.
The structure and scope of the dissertation. The structure of the dissertation consists of an introduction, three chapters, a conclusion and a list of references. The volume of the dissertation was 103 pages.
This article emphasizes the formation of norms for compensation of damage caused by internal affairs organs (hereafter - the IAO in context), the participation and importance of the IAO in tort relations as a “state organ” and “legal entity”. Moreover, the difference between the liability of the IAO for damage caused by its activities as a state organ and legal entity is explained. The obligation to compensate for damage as a result of the activity as a state organ should be paid from the state budget and the obligation to compensate for damage as a result of the activity as a legal entity from extra-budgetary funds of the internal affairs organs are grounded.
Civilian scholars` views on the issue of compensation for damage caused by illegal decisions, illegal actions (inaction) of internal affairs organs and officials are analyzed. The legislation system of foreign countries, including Germany, England, Turkey, Ukraine, the Russian Federation and a number of CIS countries is considered.
Proposals and recommendations have been developed to improve the mechanism of compensation for damage caused by the illegal application of administrative and criminal law by the internal affairs organs in our national legislation. Establishing special state fund to ensure timely and full compensation for damage caused to citizens and legal entities in the exercise of internal affairs organs and their officials have been scientifically substantiated
Objects of research: 92 immature rats with term of 30-40 days. 108 patients with asiderotic an anemia: men - 13 (12%), women - 95 (88%); middle age - 39,9 years. The control group was made by 17 healthy persons.
The purpose of work: Increase of efficiency of treatment of patients asiderotic [iron-deficiency] anemia by an induction of synthesis and a metabolism a theme in a liver.
Methods of research: clinical, biochemical, morphological, tool.
The received results and their novelty: Activization of the initial stages of synthesis is established on models alimentary asiderotic [iron-deficiency] anemia in mitochondrial and microsomal-cytosol fractions of a liver a theme, on a background to inhibit its final stage. Correction of a level malonic dialdehyde and activity of enzymes superoxide dismutase (SOD) and catalase (CAT) is revealed as a result of application of a combination of preparations maltofcr and neosclcn in mitochondrial and microsomal-cytosol fractions of a liver of experimental animals. For the first time we analyse parameters of ions of tri valent, bivalent iron both dif-feritin in mitochondrial and microsomal-cytosol fractions of a liver of experimental animals. The morphological picture of a liver at alimentary iron deficiency is submitted an anemia and correction of the revealed infringements by preparations maltofcr and ncosclcn. For the first time on the big clinico-expcrimcntal material efficiency of application of a combination of preparations maltofer and neoselen is shown.
The practical importance: Application of a combination of preparations maltofer and neoselen on a clinico-experimental material restores activity of enzymes of synthesis and disintegration a theme in hepatocyte’s, improves ferroki-nctic, hematologic parameters and promotes improvement of a functional condition of a liver. The received positive results in clinical researches have found acknowledgement (confirmation) on an experimental material that allows to recommend the given circuit of treatment железодефицитной to an anemia in clinical and outpatient conditions.
Degree of introduction and economic efficiency: Results of research are introduced into practice гематологического, therapeutic branches, and as for doctors of an initial link.
Scope: hematology, therapy.
Snake venom contains a complex mixture of bioactive components that can have profound effects on various physiological processes. The aim of this study was to investigate the influence of snake (Naja naja) venom on DNA damage in albino rats. Albino rats were injected with a controlled dosage of Naja naja venom, and the extent of DNA damage was assessed using various molecular techniques. The results revealed a significant increase in DNA damage in the venom-injected rats compared to the control group. The observed DNA damage included single-strand breaks, double-strand breaks, and oxidative damage. The findings highlight the potential genotoxic effects of snake venom on DNA integrity and suggest the importance of further research to understand the underlying mechanisms and develop appropriate therapeutic interventions.
This article studies the peculiarities of compensation for moral damage of contractual legal relations and offers proposal of improving the legislation in this sphere. It concludes that in the event that a party that has signed a contract with an individual does not fulfill contractual obligations, the victim may claim compensation not only for material damage but also for moral damage.
Purpose of the study: to study some biochemical parameters of mesenchymal-inflammatory and hypercoagulable syndromes in patients with liver damage who underwent SARS-COV2 infection.
Materials and research methods. 243 patients who had COVID-19 at the age of 18-60 were under observation. Inclusion criteria in the study were: transferred no earlier than 10 days prior to entry into the COVID-19 study; at the time of inclusion in the study PCR-negative COVID-19, negative PCR and markers of replication of hepatitis viruses. As a control group (CG), 20 healthy volunteers were examined. Enzymes were determined in the blood serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma glutamyl aminotransferase (GGT), lactate dehydrogenase (LDH), alkaline phosphatase (AP), total and direct bilirubin, albumin, ferritin, C reactive protein and complete blood count.
Research results The activity of blood liver enzymes in patients who underwent COVID-19 was significantly increased compared to CG: ALT exceeded the average values in CG by almost 10 times, AST = almost 3 times, LDH - 3 times, GGT and ALP - almost 1 .5 times. The level of bilirubin in the CG was significantly higher (p<0.001). The concentration of albumin in the peripheral blood of patients was reduced (p<0.001 significance of the difference from CG). The level of hemoglobin and erythrocytes in peripheral blood was significantly lower than in the CG (p<0.001 and p<0.05). The platelet count was reduced (p<0.001 significant difference from CG). The ESR and CRP concentrations were significantly increased compared with the CG (p<0.001 significance for both indicators).
Conclusion
In patients who have undergone COVID-19, functional changes in the liver are noted, characterized by cytolytic, cholestatic syndrome, and a decrease in protein-synthesizing function. Also, these patients have signs of redistributive anemia and sideropenia, thrombocytopenia, and persistent activity of mesenchymal-inflammatory and coagulopathic syndromes. ALT activity significantly positively correlates with the activity of systemic inflammation and hypercoagulability indices.
We have studied the morphological, morphometric parameters of the liver of white rats. Against the background of polypharmacy, when using non-steroidal anti-inflammatory drugs, it was revealed in the structure of the liver, which led to various damage to the liver parenchyma. The simultaneous use of non-steroidal anti-inflammatory drugs leads to acute or chronic liver failure in rats. The longer the therapy for chronic diseases, the more pronounced morphological changes (necrosis, fibrosis, and cholestasis).
Acute appendicitis is a common surgical emergency characterized by inflammation of the appendix. While the condition primarily affects the appendix, it can lead to systemic effects and potential complications. The liver, being an important organ involved in various metabolic processes, may be affected by the inflammatory response associated with acute appendicitis. This study aims to evaluate the impact of acute appendicitis and its complications on liver function test parameters. Liver function tests (LFTs), including alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total bilirubin (TB), and direct bilirubin (DB), were measured in patients diagnosed with acute appendicitis. The LFT results were compared between patients with uncomplicated appendicitis and those with complications such as perforation or abscess formation. Statistical analysis was performed to identify any significant differences in the liver function parameters between the two groups. The findings of this study provide insights into the potential effects of acute appendicitis and its complications on liver function, which may aid in the early detection and management of hepatic abnormalities in patients with this condition.
The features of the epidemiology, clinical picture, diagnosis and treatment of chronic diffuse liver diseases in combination with anemia are considered according to the literature on this problem.
It has been shown that anemia aggravates the course, causes progression and worsens the prognosis of chronic diffuse liver diseases. The incidence of anemia in chronic diffuse liver diseases reaches 80%. Disclosure of the mechanisms of anemia in chronic diffuse liver diseases is necessary for timely correction and prevention of the progression of both pathological changes in red blood and underlying liver disease.